Mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety IR64

Abstract

Background Rice planthoppers (main brown planthopper, Nilaparvata lugens Stål; BPH) was one of substantial threats to Asia rice production as its serious destruction and difficulties in control under field conditions. Notably, host-plant resistance was proved to be one of the effective ways to manage the pest. And stronger virulence will probably emergence when continuous use of insecticides. Therefore, more resistance genes with different resistance mechanisms were needed to be detected and then applied in the rice breeding practice. Results Resistance genes in the rice variety IR64 were evaluated considering the seedling bulk test and seedling survival rate. As a result, a locus with a large LOD score of 7.23 was found between markers RM302 and YM35 on chromosome 1. The locus explained 36.9% of phenotypic variation and was tentatively denominated Bph37 . Moreover, Bph1 was detected to be harbored by the markers RM28366 and RM463, and had the largest LOD score of 2.08, explaining 7.7% of phenotypic variance in the same mapping population. Finally, the preliminary-near-isogenic-lines (pre-NILs) carrying Bph37 exhibited significant tolerance to the insects. But no antibiotic or antixenotic effects were observed in the resistant plants when infested with the insects. Conclusions We mapped one major BPH resistance gene Bph37 in consideration of seedling survival rate and the resistance lines showed tolerance to BPH. The detected gene should be beneficial for understanding the resistance mechanism of rice to BPH and for insect-resistance rice breeding programs.

Regulation of Carbohydrate Metabolism by Trehalose-6-Phosphate Synthase 3 in the Brown Planthopper, Nilaparvata lugens

“…Locusts can pose a major threat to rice, and destruction of produce by locusts occurs in frequent bursts. Among them, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) is one of the most destructive pests of rice (Yang et al, 2019). This insect can use its probe to pierce the sheath phloem sap and absorb vascular fluids containing sucrose, amino acids, potassium, and ATP from vascular bundles (Hayashi and Chino, 1990).…”

Identification of a novel locus, BPH38(t), conferring resistance to brown planthopper (Nilaparvata lugens Stal.) using early backcross population in rice (Oryza sativa L.)

“…Although several strategies are available to manage BPH infestation, building BPH resistance in rice plants by identifying and introgressing BPH resistance genes is the most convenient and efficient strategy. To date, 37 BPH resistance genes have been identified from cultivated and wild species of Oryza : BPH1 , BPH2 , BPH3 , BPH4, BPH5, BPH6, BPH7, BPH8, BPH9, BPH10, BPH11 (t) , BPH12 (t) ,BPH12, BPH13 (t), BPH14 , BPH15 , BPH16 (t), BPH17, BPH18, BPH19 (t) , BPH20, BPH21, BPH22 (t) , BPH23 (t) ,BPH24 (t) , BPH25 (t) , BPH26 (t), BPH27 , BPH28, BHP29,BPH30, BPH31, BPH32,BPH33 , BPH3 and BPH35, BPH36 and BPH37 (Khush et al 1985; Kabis and Khush 1988; Nemoto et al 1989; Ishii et al 1994; Murata et al 1998; Hirabayashi et al 1998; Renganayaki et al 2002; Yanget al 2002; Sharma et al 2003; Yang et al 2004; Hirabayashi et al 2004; Sun et al 2005; Chang-Chao et al 2006; Chen et al 2006; Jena et al 2006; Sai Harini et al 2010; Jairin et al 2007, 2010; Li et al 2019; Ram et al 2008; Rahman et al 2009; Du et al 2009; Qiu et al 2010; Deen et al 2010; Qiu et al 2012; Myint et al 2012; Huang et al 2013; Wu et al 2014; Wang et al 2015; Wang et al 2018; Ren et al 2016; Prahalada et al 2017; Kumar et al 2018; Naik et al 2018; Yang et al 2019; Yuexiong et al 2019). Among these, only eight genes ( BPH14, BPH17, BPH18, BPH26, BPH29, BPH9, BPH32, BPH31 and BPH6 ) were cloned and characterized (Du et al 2009; Tamura et al 2014; Liu et al 2015; Wang et al 2015; Ji et al 2016; Ren et al 2016; Zhao et al 2016; Guo et al 2018).…”

“…To date, 37 BPH resistance genes have been reported from different resistance sources (Li et al 2019; Yang et al 2019; Yuexiong et al 2019). Notably, the majority of the BPH resistance genes are mapped on six of the 12 chromosomes (2, 3, 4, 6, 11, and 12) and, reportedly, four clusters of BPH resistance loci are located on three chromosomes.…”

“…rufipogon accession GX2183) were used to map BPH36 and BPH27 on chromosome 4 [ BPH27 already reported by Huang et al (2013) on Chromosome 4], and BPH36 were fine mapped by using backcross population (Li et al 2019). Recently, Yang et al (2019) mapped a novel QTL BPH37 by using F 2:3 population derived from KWQZ/IR64 on chromosome 1, between RM 302 and YM35 markers. It is already reported that IR64 is harboring a major resistant gene BPH1 on chromosome 12 and minor QTLs associated with BPH resistance, whereas BPH37 is a second major resistance locus was reported on chromosome 1.…”

Applications of Biotechnology for Characterization of Plants and Pests as the Key Components of Plant Protection and Production Strategies: A Review

“…Transgene Insertion Sites (Salvo-Garrido et al, 2004) Genetic and physical mapping (Simpson and Luna-Martinez, 2011) Maize resistance against borer (Sadder and Weber, 2002) QTL Mapping molecular breeding (Khan, 2015); Mulualem and Bekeko, 2015;Veeresha et al, 2015;Sehgal et al, 2016;Xu et al, 2017) resistance to multiple races of loose smut in wheat (Kumar et al, 2018); resistance to late blight in tomato (Panthee et al, 2017) Planthopper resistance in rice (Ren et al, 2004;Yang et al, 2019),…”