Mapping and Analysis of QTL for Early Maturity Trait in Tetraploid Potato (Solanum tuberosum L.)

Abstract

16Maturity is one of the important traits of potato. In order to get the genetic segment of potato early maturity trait, 17 a tetraploid potato maturity segregation population of Zhongshu 19 × Zhongshu 3 was used for genetic analysis 18 through the combination of high throughput simplified genome sequencing (2b-RAD) and bulked segregation 19 analysis (BSA). A genetic segment related to the early maturity trait at the 3.7~4.2 Mb locus on the short arm of 20 chromosome 5 was obtained and eight markers were developed based on this segment, while five of them were 21 closely linked to the early maturity trait loci. Moreover, 42 SSR markers were developed based on the reference 22 sequence of DM. Finally, a genetic map of chromosome 5 contained 50 markers was constructed using the 23 Tetraploidmap software. The total map length was 172 cM with an average genetic distance of 3.44 cM. 24Combining with phenotypic data of the segregation population, we mapped the early maturity trait QTL with the 25 contribution of 33.55% on the short arm of chromosome 5, located at 84cM between the flanking markers 26 SSR5-85-1 and SCAR5-8 with the physical interval of 471kb. Gene annotation showed that there exist 34 genes 27 in this region, 12 of them are unknown function. Among the other 22 annotated genes, E3 ubiquitin ligase gene 28 PUB14 may be related to maturity and regulate tuber formation. Our fine mapping of the early maturity QTL 29 made a solid foundation for cloning of the early maturity controlled gene or genes. 30 Key words：potato ·genetic segment · early maturity trait · marker development · QTL mapping 31Author contribution statement 33 GL and LJ conceived the project, provided idea and designed the whole experiments; XL and JZ conceived the 34 project and performed the molecular marker development and analysis; JX contributed to the maturity 35 phenotyping of the mapping population; SD performed the parents crossing of the mapping population; CB and 36 JH performed the field management of potato materials. All authors read and approved the final manuscript. 37 Key message 38Early maturity site was mapped using a tetraploid potato segregation population derived from cv. Zhongshu 19 39 and Zhongshu 3. One major QTL with 33.55% contribution to early maturity was fine mapped in physical 40 interval of 471kb on chromosome 5. potato (2n = 4x = 48) is highly heterozygous, with high frequency of genetic recombination and 87 severe recession after self-fertilization (Manrique-Carpintero et al. 2018). The molecular marker is difficult to 88 develop. The diploid potato chromosome number is small, the genetic background is relatively simple, and it is 89 easy to carry out genetic analysis and operation. Therefore, the development of potato markers and genetic map 90 construction is mainly at the diploid level, and is done mainly with the use of the traditional QTL mapping 91 analysis method, whereas fewer studies are conducted on tetraploid materials. Although most of the studies have 92 mapped QTLs on chromosome 5, there are no m...

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