Mapping an aphid resistance gene in soybean [Glycine max (L.) Merr.] P746

Liang, Xiao; Zhong, Yunpeng; Wang, B.; Wu, Tianlong

doi:10.4238/2014.november.7.2

Cited by 5 publications

(6 citation statements)

References 31 publications

(41 reference statements)

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“…Previous studies on the response of soybean to soybean aphid, which used high throughput technology, focused on soybean genotypes with antibiosis harboring Rag1 and Rag2 [19][20][21][22]. Here, we revealed transcriptome profiles of both antibiotic and antixenotic genotypes harboring resistant genes [16,27] 24 h, 48 h, and 96 h after aphid infestation. Several results were also observed in resistant or susceptible genotypes of other plants under insect attack or bacterial infection, which indicated the general response of plant to biotic stress.…”

Section: Discussionmentioning

confidence: 91%

“…Three soybean genotypes with contrasting response to Aphis glycine were used in this study: an aphid-susceptible soybean cultivar Dongnong47, the antibiotic genotype P746, and the antixenotic genotype P203 [16,27]. Seeds from each genotype were planted into pots (8 cm deep and 10 cm in diameter) filled with potting media (34% peat, 31% perlite, 31% vermiculite, and 4% soil mix).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Soybean germplasms exhibiting antibiosis, antixenosis, and tolerance have already been identified [ 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. Several resistance genes were mapped in some of those germplasms, such as Rag1 , Rag2 , and Rag_P746 , which confers antibiosis [ 14 , 15 , 16 ], and Rag3 , Rag4 , and Rag_P203 , encoding for antixenotic resistance [ 7 , 8 , 16 ]. The mechanics contributing to the resistance in legumes include structural defenses, secondary metabolites, and signaling components [ 6 ], but the underlying genetic basis of the aphid–plant interaction is still not well understood [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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A Genome-Wide View of Transcriptional Responses during Aphis glycines Infestation in Soybean

Yao

Yang

et al. 2020

IJMS

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Soybean aphid (Aphis glycines Matsumura) is one of the major limiting factors in soybean production. The mechanism of aphid resistance in soybean remains enigmatic as little information is available about the different mechanisms of antibiosis and antixenosis. Here, we used genome-wide gene expression profiling of aphid susceptible, antibiotic, and antixenotic genotypes to investigate the underlying aphid–plant interaction mechanisms. The high expression correlation between infested and non-infested genotypes indicated that the response to aphid was controlled by a small subset of genes. Plant response to aphid infestation was faster in antibiotic genotype and the interaction in antixenotic genotype was moderation. The expression patterns of transcription factor genes in susceptible and antixenotic genotypes clustered together and were distant from those of antibiotic genotypes. Among them APETALA 2/ethylene response factors (AP2/ERF), v-myb avian myeloblastosis viral oncogene homolog (MYB), and the transcription factor contained conserved WRKYGQK domain (WRKY) were proposed to play dominant roles. The jasmonic acid-responsive pathway was dominant in aphid–soybean interaction, and salicylic acid pathway played an important role in antibiotic genotype. Callose deposition was more rapid and efficient in antibiotic genotype, while reactive oxygen species were not involved in the response to aphid attack in resistant genotypes. Our study helps to uncover important genes associated with aphid-attack response in soybean genotypes expressing antibiosis and antixenosis.

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Section: Discussionmentioning

confidence: 91%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Genome-Wide View of Transcriptional Responses during Aphis glycines Infestation in Soybean

Yao

Yang

et al. 2020

IJMS

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“…The study revealed that QTL_13_1 and QTL_13_2 were mapped very close to already reported loci, Rag2 and Rag4 , respectively (Jun et al 2013 ). Xiao et al ( 2014 ) mapped R_P746 between SSRs, Satt334, and Satt335 , on chromosome 13 in a P746 × Dongnong 47 derived F 2:3 mapping population comprising of 312 individuals. These linked markers will prove to be valuable in MAS-based aphid resistance breeding program in soybean.…”

Section: Insect Resistance Qtls Identified In Major Field Cropsmentioning

confidence: 99%

Molecular mechanisms, genetic mapping, and genome editing for insect pest resistance in field crops

et al. 2022

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Key message Improving crop resistance against insect pests is crucial for ensuring future food security. Integrating genomics with modern breeding methods holds enormous potential in dissecting the genetic architecture of this complex trait and accelerating crop improvement. Abstract Insect resistance in crops has been a major research objective in several crop improvement programs. However, the use of conventional breeding methods to develop high-yielding cultivars with sustainable and durable insect pest resistance has been largely unsuccessful. The use of molecular markers for identification and deployment of insect resistance quantitative trait loci (QTLs) can fastrack traditional breeding methods. Till date, several QTLs for insect pest resistance have been identified in field-grown crops, and a few of them have been cloned by positional cloning approaches. Genome editing technologies, such as CRISPR/Cas9, are paving the way to tailor insect pest resistance loci for designing crops for the future. Here, we provide an overview of diverse defense mechanisms exerted by plants in response to insect pest attack, and review recent advances in genomics research and genetic improvements for insect pest resistance in major field crops. Finally, we discuss the scope for genomic breeding strategies to develop more durable insect pest resistant crops.

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“…The identification of molecular markers associated with resistance is helpful for developing resistant cultivars because phenotyping soybean for nematode resistance is time-consuming and costly (Kadam et al, 2016). Transgenic approaches have not been reported for soybean resistance to nematodes; however, marker assisted selection has been broadly studied and reported in soybean against other pests (Concibido et al, 2004;Xiao et al, 2014). In this context, strategies used to identify molecular markers for nematode resistance using those based on plant resistance genes (R genes) and the so-called resistance gene analogs (RGA) constitute one alternative that has not yet been employed in soybean.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of soybean genotype resistance to Heterodera glycines and Meloidogyne species via resistance gene analogs

et al. 2016

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ABSTRACT. Nematodes are important pests of soybean throughout the world and cause high yield losses. As a control strategy, the identification of resistance genes is an important aim of breeding studies. Plants possess resistance genes (R), which are responsible for the recognition of pathogens and activation of the defense system. R genes and resistance gene analogs (RGAs) possess conserved domains, from which nucleotide-binding site is the most common. Using degenerate primers originating from these domains, it is possible to identify and isolate sequences of R and RGA genes. In this study, soybean genotypes resistant to the nematodes Heterodera glycines, Meloidogyne incognita, M. javanica, and M. enterolobii were compared by the use of RGAs and simple sequence repeat (SSR) markers. Forty-six soybean genotypes were studied, including plant introductions (PIs), commercial crops, and source of resistance genotypes. Thirteen combinations of RGA primers and different SSRs linked to QTLs were used to confirm resistance to soybean cyst nematodes (SCN). Fragments associated with resistance to the studied nematodes were amplified in the source of resistance and PI genotypes. RGA markers were efficient at distinguishing groups of genotypes that were resistant and susceptible to Meloidogyne spp and SCN. Combinations of specific primers were identified through their ability to amplify nucleotide sequences from possible resistance candidate genes. SSR markers contributed to the analysis of SCN race specificity, showing that the QTLs identified by these markers are distinct from those identified by RGA markers.

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Mapping an aphid resistance gene in soybean [Glycine max (L.) Merr.] P746

Cited by 5 publications

References 31 publications

A Genome-Wide View of Transcriptional Responses during Aphis glycines Infestation in Soybean

A Genome-Wide View of Transcriptional Responses during Aphis glycines Infestation in Soybean

Molecular mechanisms, genetic mapping, and genome editing for insect pest resistance in field crops

Comparative analysis of soybean genotype resistance to Heterodera glycines and Meloidogyne species via resistance gene analogs

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