Map-based cloning and characterization of a gene controlling hairiness and seed coat color traits in Brassica rapa

Zhang, Jiefu; Lu, Ying; Yuan, Yuxiang; Zhang, Xiaowei; Geng, Jianfeng; Chen, Yu; Cloutier, Sylvie; McVetty, P. B. E.; Li, Genyi

doi:10.1007/s11103-008-9437-y

Cited by 107 publications

(89 citation statements)

References 30 publications

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“…Recently, a pubescence locus was also been mapped on LG A6 and its responsible gene was show to be a homolog of the Arabidopsis TRANSPARENT TESTA GLABRA 1 (TTG1) gene (Zhang et al 2009). In contrast, Li et al (2009) have recently proposed the involvement of a homolog of the GLABRA1 (GL1) gene in pubescence using a different B. rapa population.…”

Section: Comparison Of the Pubescence Qtl Among Different Mapsmentioning

confidence: 99%

“…Pubescence in the Brassica species is an important trait for plant defense against insects (Ågren and Schemske 1992). The presence/absence of pubescence is controlled by a single gene in many cases (Teutonico and Osborn 1994, Song et al 1995, Nozaki et al 1997, Lou et al 2007, Zhang et al 2009, whereas this trait is also reported as a quantitative trait (Ågren and Schemske 1992). However, the degree of pubescence may be controlled by polygenes (Song et al 1995).…”

Section: Introductionmentioning

confidence: 99%

“…However, the degree of pubescence may be controlled by polygenes (Song et al 1995). Recently, a gene controlling pubescence and seed-coat color has been isolated by mapbased cloning (Zhang et al 2009). The leaf lobe (leaf margin) exhibits great variation in B. rapa cultivars and may be important, especially for leafy vegetables.…”

Section: Introductionmentioning

confidence: 99%

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Detection of quantitative trait loci controlling morphological traits in Brassica rapa L.

et al. 2010

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The Brassica species displays great variation with regard to morphology. However, the genetic mechanisms that underlie this variability are mostly unknown. We generated 188 F 2 plants derived from a cross between a Chinese cabbage and a vegetable turnip, and scored several morphological traits (head formation, leaf lobe, pubescence, and turnip formation) twice (2005 and 2007) in two subsets of 94 individuals. With the exception of pubescence, we did not find any typical discrete segregation of these traits in F 2 plants, suggesting that they are controlled by multiple genes. We constructed a linkage map to conduct quantitative trait locus (QTL) analysis, and detected eight and 14 QTLs in 2007 and 2005, respectively, for head formation, leaf lobe, pubescence, turnip size, and turnip weight. The amplified fragment length polymorphism (AFLP) fragments linked to the QTLs were converted into sequence-tagged site (STS) markers. The QTLs detected here and their linkage markers may provide useful information for the selection of traits during the breeding of Chinese cabbage and turnip cultivars.

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Section: Comparison Of the Pubescence Qtl Among Different Mapsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of quantitative trait loci controlling morphological traits in Brassica rapa L.

et al. 2010

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“…In another study, Lou et al (2007) detected 10 QTLs for leaf traits using three different mapping populations. By synteny analysis of QTL regions of B. rapa with the Arabidopsis genome, Li et al (2009Li et al ( , 2013 identified the leaf lobe depth and leaf hairiness genes, BRASSICA RAPA GIBBERELLIN 20-OXIDASE 3 (BrGA20OX3) AND BRASSICA RAPA GLABRA1, and Zhang et al (2009) successfully cloned a BRASSICA RAPA TRANSPARENT TESTA GLABRA1 gene controlling leaf hairiness and seed coat color. Genetical genomics or expression quantitative trait locus (eQTL) mapping entails a QTL analysis of highthroughput transcript expression patterns in a genotyped population.…”

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confidence: 99%

Genetic Dissection of Leaf Development in Brassica rapa Using a Genetical Genomics Approach

et al. 2014

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The paleohexaploid crop Brassica rapa harbors an enormous reservoir of morphological variation, encompassing leafy vegetables, vegetable and fodder turnips (Brassica rapa, ssp. campestris), and oil crops, with different crops having very different leaf morphologies. In the triplicated B. rapa genome, many genes have multiple paralogs that may be regulated differentially and contribute to phenotypic variation. Using a genetical genomics approach, phenotypic data from a segregating doubled haploid population derived from a cross between cultivar Yellow sarson (oil type) and cultivar Pak choi (vegetable type) were used to identify loci controlling leaf development. Twenty-five colocalized phenotypic quantitative trait loci (QTLs) contributing to natural variation for leaf morphological traits, leaf number, plant architecture, and flowering time were identified. Genetic analysis showed that four colocalized phenotypic QTLs colocalized with flowering time and leaf trait candidate genes, with their cis-expression QTLs and cisor trans-expression QTLs for homologs of genes playing a role in leaf development in Arabidopsis (Arabidopsis thaliana). The leaf gene BRASSICA RAPA KIP-RELATED PROTEIN2_A03 colocalized with QTLs for leaf shape and plant height; BRASSICA RAPA ERECTA_A09 colocalized with QTLs for leaf color and leaf shape; BRASSICA RAPA LONGIFOLIA1_A10 colocalized with QTLs for leaf size, leaf color, plant branching, and flowering time; while the major flowering time gene, BRASSICA RAPA FLOWERING LOCUS C_A02, colocalized with QTLs explaining variation in flowering time, plant architectural traits, and leaf size. Colocalization of these QTLs points to pleiotropic regulation of leaf development and plant architectural traits in B. rapa.Six Brassica species are cultivated worldwide: three diploid Brassica species, Brassica rapa (A genome; n = 10), Brassica nigra (B genome; n = 8), and Brassica oleracea (C genome; n = 9), and three amphidiploids, Brassica juncea (AB; n = 18), Brassica napus (AC; n = 19), and Brassica carinata (BC; n = 17), derived by spontaneous hybridization among the three diploid species (Nagaharu, 1935). All diploid Brassica species have undergone a whole-genome triplication since their divergence from Arabidopsis (Arabidopsis thaliana; Lysak et al., 2005;Town et al.,

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“…The SRAP technique was used for several purposes, including genetic map construction (Li et al, 2003;Gao et al, 2007;Sun et al, 2007), QTL mapping Fu et al, 2007;Zhang et al, 2009b), gene tagging and cloning (Rahman et al, 2007;Yi et al, 2008;Zhang et al, 2009a), and genetic diversity (Riaz et al, 2001;Budak et al, 2004a,b;Vandemark et al, 2006).…”

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confidence: 99%

Molecular phylogeny of Lathyrus species: insights from sequence-related amplified polymorphism markers

et al. 2016

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ABSTRACT. Sequence-related amplified polymorphism (SRAP) markers were used to evaluate the intra-and interspecific variation among 40 Lathyrus genotypes (four species) (Fabaceae). Ten SRAP primer combinations resulted in a total of 94 bands, and they exhibited high interspecific variability. The genetic differentiation among Lathyrus, estimated using AMOVA, was highly significant. The results indicated that 58% of the total genetic variation existed among species, and 42% of the differentiation was within species. This was explained by the high level of genome conservation of these species as well as the recent and slow evolution of this genus. These results were confirmed by the topology of the neighbor-joining cladogram and the results of the principal coordinate analysis. Our data support previous results based on seed protein diversity. These results make SRAP markers choice markers for the study of functional polymorphism that is directly related to the transcriptomic data. The SRAP markers used in this study provide an accurate picture of the population structure within Lathyrus germplasm, which is critically important information for the design of genetic diversity and structure analyses. Moreover, further extensive studies are necessary to fully examine other Lathyrus species and tests that adopt the SRAP technique to enrich the Lathyrus library for next-generation sequencing, thus providing a potent protocol for the study of polymorphism.

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Map-based cloning and characterization of a gene controlling hairiness and seed coat color traits in Brassica rapa

Cited by 107 publications

References 30 publications

Detection of quantitative trait loci controlling morphological traits in Brassica rapa L.

Detection of quantitative trait loci controlling morphological traits in Brassica rapa L.

Genetic Dissection of Leaf Development in Brassica rapa Using a Genetical Genomics Approach

Molecular phylogeny of Lathyrus species: insights from sequence-related amplified polymorphism markers

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