Mannose prevents lipopolysaccharide-induced acute lung injury in rats

Xu, Xiao; Xie, Qiang-min; Shen, Yihong; Jiang, Juan-xia; Chen, Y. Y.; Yao, Hongwei; Zhou, Jiarong

doi:10.1007/s00011-007-7037-y

Cited by 36 publications

(27 citation statements)

References 33 publications

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“…SOD was an antioxidant enzyme with scavenging the superoxide free radical, and the decrease in SOD activity reflected the enhancement of oxidizing reaction [16]. Our results showed that the SOD activity in the lung tissue was dramatically decreased by LPS.…”

Section: Discussionmentioning

confidence: 79%

The Effects of the Combination of Sodium Ferulate and Oxymatrine on Lipopolysaccharide-Induced Acute Lung Injury in Mice

Yuan

Wang

et al. 2012

Inflammation

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In our previous study, the remarkable analgesic and anti-inflammatory effects of the combination of sodium ferulate (SF) and oxymatrine (OMT) had been found. In this study, we investigated the effect of the combination of SF and OMT on acute lung injury using lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting and the protein concentration in the bronchoalveolar lavage fluid (BALF) were measured. The animal lung edema degree was evaluated by wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators including C-reactive protein (CRP) and tumor necrosis factor-α (TNF-α) were assayed by enzyme-linked immunosorbent assay method. The data showed that treatment with the combination of SF and OMT markedly attenuated inflammatory cell numbers and protein concentration in the BALF and improved SOD activity and inhibited MPO activity compared to LPS group. Moreover, the combination significantly inhibited the production of CRP and TNF-α in lung homogenate. The histological changes of the lungs were also more significantly improved by the combination. At the same dose, the obvious protective effect was not found in SF or OMT-treated alone group except that the protein concentration slightly decreased in SF group. The results indicated that the combination SF and OMT had a protective effect on LPS-induced ALI in mice, and the effect was much better than that of SF or OMT used alone.

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Section: Discussionmentioning

confidence: 79%

The Effects of the Combination of Sodium Ferulate and Oxymatrine on Lipopolysaccharide-Induced Acute Lung Injury in Mice

Yuan

Wang

et al. 2012

Inflammation

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“…pretreatment with D-mannose, also characterized to exert anti-inflammatory effects [76,77], significantly altered the efficacy of a subsequent i.t. pDNA-IL-10 injection in that complete and enduring suppression of bilateral allodynia was observed at pDNA-IL-10 doses five-fold lower than previously documented (Figure 2) [24,46].…”

Section: Discussionmentioning

confidence: 99%

Improvement of spinal non-viral IL-10gene delivery by D-mannose as a transgene adjuvant to control chronic neuropathic pain

Dengler

Alberti

Bowman

et al. 2014

J Neuroinflammation

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BackgroundPeri-spinal subarachnoid (intrathecal; i.t.) injection of non-viral naked plasmid DNA encoding the anti-inflammatory cytokine, IL-10 (pDNA-IL-10) suppresses chronic neuropathic pain in animal models. However, two sequential i.t. pDNA injections are required within a discrete 5 to 72-hour period for prolonged efficacy. Previous reports identified phagocytic immune cells present in the peri-spinal milieu surrounding the i.t injection site that may play a role in transgene uptake resulting in subsequent IL-10 transgene expression.MethodsIn the present study, we aimed to examine whether factors known to induce pro-phagocytic anti-inflammatory properties of immune cells improve i.t. IL-10 transgene uptake using reduced naked pDNA-IL-10 doses previously determined ineffective. Both the synthetic glucocorticoid, dexamethasone, and the hexose sugar, D-mannose, were factors examined that could optimize i.t. pDNA-IL-10 uptake leading to enduring suppression of neuropathic pain as assessed by light touch sensitivity of the rat hindpaw (allodynia).ResultsCompared to dexamethasone, i.t. mannose pretreatment significantly and dose-dependently prolonged pDNA-IL-10 pain suppressive effects, reduced spinal IL-1β and enhanced spinal and dorsal root ganglia IL-10 immunoreactivity. Macrophages exposed to D-mannose revealed reduced proinflammatory TNF-α, IL-1β, and nitric oxide, and increased IL-10 protein release, while IL-4 revealed no improvement in transgene uptake. Separately, D-mannose dramatically increased pDNA-derived IL-10 protein release in culture supernatants. Lastly, a single i.t. co-injection of mannose with a 25-fold lower pDNA-IL-10 dose produced prolonged pain suppression in neuropathic rats.ConclusionsPeri-spinal treatment with D-mannose may optimize naked pDNA-IL-10 transgene uptake for suppression of allodynia, and is a novel approach to tune spinal immune cells toward pro-phagocytic phenotype for improved non-viral gene therapy.

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“…18 In brief, the left lungs were lavaged with 2 ml PBS. A microvascular clamp was used to occlude the right hilum before lavage.…”

Section: Analysis Of Pulmonary Microvascular Permeability and Pulmonamentioning

confidence: 99%

Ischemic post-conditioning attenuates acute lung injury induced by intestinal ischemia–reperfusion in mice: role of Nrf2

Meng

Cao²,

et al. 2016

Laboratory Investigation

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Intestinal ischemic post-conditioning (IPo) protects against lung injury induced by intestinal ischemia-reperfusion (IIR) partly through promotion of expression and function of heme oxygenase-1 (HO-1). NF-E2-related factor-2 (Nrf2) is a key transcription factor that interacts with HO-1 and regulates antioxidant defense. However, the role of Nrf2 in IPo protection of IIR-induced pulmonary injury is not completely understood. Here we show that IPo significantly attenuated IIR-induced lung injury and suppressed oxidative stress and systemic inflammatory responses. IPo also increased the expression of both Nrf2 and HO-1. Consistently, the beneficial effects of IPo were abolished by ATRA and Brusatol, potent inhibitors of Nrf2. Moreover, the Nrf2 agonist t-BHQ showed similar activity as IPo. Taken together, our data suggest that Nrf2 activity, along with HO-1, plays an important role in the protective effects of IPo against IIR-induced acute lung injury.

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Mannose prevents lipopolysaccharide-induced acute lung injury in rats

Abstract: Mannose is effective in reducing LPS-induced ALI.

Cited by 36 publications

References 33 publications

The Effects of the Combination of Sodium Ferulate and Oxymatrine on Lipopolysaccharide-Induced Acute Lung Injury in Mice

The Effects of the Combination of Sodium Ferulate and Oxymatrine on Lipopolysaccharide-Induced Acute Lung Injury in Mice

Improvement of spinal non-viral IL-10gene delivery by D-mannose as a transgene adjuvant to control chronic neuropathic pain

Ischemic post-conditioning attenuates acute lung injury induced by intestinal ischemia–reperfusion in mice: role of Nrf2

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