Mannan from the extracellular surface ofCandida albicans Berkhout

Šikl, D.; Masler, L.; Bauer, Štefan

doi:10.1007/bf02152149

Cited by 8 publications

(5 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Addition of tunicamycin to 500 mM-galactose-grown cultures at the onset of the stationary phase of growth decreased yeast adhesion and increased yeast sensitivity to spheroplast formation, presumably by inhibiting synthesis of the fibrillar surface layer (Douglas & McCourtie, 1983). Removal of surface mannoprotein from C. albicans by warm water washes has been reported by Sikl et al (1964). The same authors (Masler et al, 1966) have also described the isolation from yeast culture supernatants of polysaccharide-protein complexes similar in composition to EP prepared in this study.…”

Section: Discussionsupporting

confidence: 58%

Extracellular Polymer of Candida albicans: Isolation, Analysis and Role in Adhesion

McCourtie¹,

Douglas²

1985

Microbiology

102

View full text Add to dashboard Cite

~ ~~ ~~Extracellular polymeric material (EP) was isolated from culture supernatants of Candida albicans grown on carbon sources (50 mM-glucose, 500 mM-SUCrOSe or 500 mM-galactose) known to promote yeast adhesion to different extents. Galactose-grown yeasts, which are the most adherent, produced more EP than sucrose-grown organisms, particularly after incubation for 5 d, while glucose-grown yeasts (the least adherent) gave the lowest yield. EP produced on all three carbon sources was of similar composition and contained carbohydrate (65 to 82%; mannose with some glucose), protein (7 %), phosphorus (0.5 %) and glucosamine (1 -5 %).Serological studies indicated that these EP preparations were immunologically identical but that galactose-grown yeasts had more antigenic determinants than sucrose-grown organisms while glucose-grown yeasts had the fewest determinants. Antigenic differences were apparent between EP preparations of some strains of C. albicans. Pretreatment of acrylic strips with EP to form a polymeric coating promoted yeast adhesion to the acrylic surface, but similar pretreatment of buccal epithelial cells with EP inhibited subsequent yeast adhesion. These results indicate that EP originates from the cell surface of C. albicans and that it contains the surface component(s), probably mannoprotein in nature, responsible for yeast adhesion.

show abstract

Section: Discussionsupporting

confidence: 58%

Extracellular Polymer of Candida albicans: Isolation, Analysis and Role in Adhesion

McCourtie¹,

Douglas²

1985

Microbiology

102

View full text Add to dashboard Cite

show abstract

“…One of the first ways in which such diagnostic antigens were obtained was by the concentration of culture filtrates. During the nineteen sixties, yeast-phase cultures, incubated for up to 4 weeks, were widely used to obtain antigens (Louria et al, 1963;Mankowski, 1968;Masler et al, 1966;Sikl et al, 1964). These antigens showed cross-reactivity with antigenic preparations from the cell wall and the cytoplasm, and may therefore be considered as autolytic products rather than secreted components (Masler et al, 1966;Tsachdjian et al, 1967).…”

Section: Discussionmentioning

confidence: 99%

“…As a first step towards identifying these antigens, we believe that compounds released by C. albicans in culture media should be investigated. Several groups have shown the presence of extracellular material in culture filtrates from yeast-phase organisms, grown on complex media, and incubated for 7 d or more (Louria et al, 1963;Mankowski, 1968;Masler et at., 1966;Sikl et al, 1964). Torosantucci et al (1991) have studied the molecular composition and antigenicity of the concanavalin A (Con A)-reactive mannoproteins released from C. albicans, but there is very little information about the proteins and polysaccharide components released into culture media.…”

Section: Introductionmentioning

confidence: 99%

Candida albicans exocellular antigens released into a synthetic culture medium: characterization and serological response in rabbits

Aguiar¹,

Baquero²,

Jones

1993

Journal of General Microbiology

View full text Add to dashboard Cite

Different exocellular extracts were isolated by concentrating the supernatants of yeast-and mycelial-phase Cundidu ulbicans cultures incubated in a synthetic medium. The only difference between the extracts obtained from the two phases was the presence in those obtained from mycelial cultures of a polysaccharide-rich, high-molecularmass component, migrating in SDS-polyacrylamide gels at a position that would correspond to proteins with molecular masses of 245-265 kDa. The electrophoretic band patterns obtained before and after concanavalin ASepharose 4B affinity column treatments confirmed that the 245-265 kDa band was the only one of mannoprotein nature. The extract obtained from 24 h mycelial-phase culture (EA) was selected as the exocellular antigen for this work. The dry weight of EA obtained from 1 litre of culture medium was 30 mg; it contained 53% carbohydrate (18-3 YO glucose and 21.7 % mannose measured by gas-liquid chromatography) and 10 % protein. Rabbit antisera against EA were absorbed with yeast-phase organisms and used to stain Western blots of gels loaded with EAs. These antisera clearly recognized bands in the 21,33 and 44 kDa areas. The antiserum obtained was employed to develop a double-antibody enzyme-linked immunosorbent assay for measuring EA concentrations in a culture medium. Most of the EA was released during the exponential phase of growth.

show abstract

“…Antigenic components released by C. albicans cells may be of potential utility as specific marker antigens for the serodiagnosis of systemic candidiasis. During the 1960s, yeast phase cultures incubated for up to 4 weeks were widely used to obtain soluble antigens (170,174,182,276). These antigens crossreacted with antigen preparations from the cell wall and the cytoplasm and may therefore be considered autolytic products rather than secreted components (182,291).…”

Section: Secreted Proteinsmentioning

confidence: 99%

Serologic Response to Cell Wall Mannoproteins and Proteins ofCandida albicans

et al. 1998

View full text Add to dashboard Cite

SUMMARY The cell wall of Candida albicans not only is the structure in which many biological functions essential for the fungal cells reside but also is a significant source of candidal antigens. The major cell wall components that elicit a response from the host immune system are proteins and glycoproteins, the latter being predominantly mannoproteins. Both the carbohydrate and protein moieties are able to trigger immune responses. Although cell-mediated immunity is often considered to be the most important line of defense against candidiasis, cell wall protein and glycoprotein components also elicit a potent humoral response from the host that may include some protective antibodies. Proteins and glycoproteins exposed at the most external layers of the wall structure are involved in several types of interactions of fungal cells with the exocellular environment. Thus, coating of fungal cells with host antibodies has the potential to influence profoundly the host-parasite interaction by affecting antibody-mediated functions such as opsonin-enhanced phagocytosis and blocking the binding activity of fungal adhesins for host ligands. In this review, the various members of the protein and glycoprotein fraction of the C. albicans cell wall that elicit an antibody response in vivo are examined. Although a number of proteins have been shown to stimulate an antibody response, for some of these species the response is not universal. On the other hand, some of the studies demonstrate that certain cell wall antigens and anti-cell wall antibodies may be the basis for developing specific and sensitive serologic tests for the diagnosis of candidasis, particularly the disseminated form. In addition, recent studies have focused on the potential for antibodies to cell wall protein determinants to protect the host against infection. Hence, a better understanding of the humoral response to cell wall antigens of C. albicans may provide the basis for the development of (i) effective procedures for the serodiagnosis of disseminated candidiasis and (ii) novel prophylactic (vaccination) and therapeutic strategies for the management of this type of infection.

show abstract

Mannan from the extracellular surface ofCandida albicans Berkhout

Cited by 8 publications

References 11 publications

Extracellular Polymer of Candida albicans: Isolation, Analysis and Role in Adhesion

Extracellular Polymer of Candida albicans: Isolation, Analysis and Role in Adhesion

Candida albicans exocellular antigens released into a synthetic culture medium: characterization and serological response in rabbits

Serologic Response to Cell Wall Mannoproteins and Proteins ofCandida albicans

Contact Info

Product

Resources

About