Mannan estimation as a measure of the growth of<i>Candida albicans</i>

Pike, Ian; Evans, E. G. V.; Carney, J. A.

doi:10.1080/02681219180000151

Cited by 4 publications

(1 citation statement)

References 20 publications

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“…In microplates, yeast growth is seen by adding an acidic indicator such as phenol red (8) to the culture medium. Measurement of the amounts of different extracellular matrix components such as mannan (27) or chitin (28) also allows yeast growth to be analyzed. In recent assays aimed at standardizing evaluation methods (1,11,12,25,26,30), it seems that the technique of growth reading chosen also plays a role in limiting the reproducibility of the assay.…”

Section: Discussionmentioning

confidence: 99%

New in vitro assay based on glucose consumption for determining intraconazole and amphotericin B activities against Aspergillus fumigatus

Garrigues

Fontenay

Linas

et al. 1994

Antimicrob Agents Chemother

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We developed a new in vitro method of evaluating antifungal molecules. Fungal growth was determined by measuring glucose consumption, the only carbon source in a synthetic medium. First, the growth of 12Aspergilus fiumigatus strains was studied. Glucose consumption was an excellent indicator of fungal growth.Second, the partial inhibition of growth was calculated in terms of the 90%o or 50% inhibitory concentration for the 12 strains after treatment with itraconazole and amphotericin B. With a 3-day incubation time, the calculated 90% and 50%v inhibitory concentrations agreed with those obtained by a macromethod and with those reported in previous publications. Therefore, it is in this direction that we developed a new in vitro evaluation test in which partial inhibition was easily quantifiable for the filamentous fungi. In the presence of two antifungal agents, itraconazole and amphotericin B, growth inhibition corresponded to a decrease in glucose consumption. Fungal growth was evaluated in a synthetic medium by measuring the kinetics of glucose degradation. MATERIALS AND METHODSStrains. Twelve strains of Aspergillus fumigatus were tested: 11 were isolated from patients and 1 was isolated from an aeromycological survey.The origins of the pathogenic strains were diverse: patients with hepatic transplants (strains 8, 11), lung transplants (strains 6 and 10), cystic fibrosis (strains 4, 5, and 7), lung aspergilloma with antecedent tuberculosis (strain 3), and colonization (strains 1 and 2) and patients receiving corticotherapy (strain 12). Strain 9 was isolated during an aeromycological survey.The strains were conserved at -70°C in a solution of glycerol and distilled water 1:9 (vol/vol). They were maintained by serial passage on a Sabouraud agar medium (Sanofi Diagnostics Pasteur, Marnes la Coquettes, France) containing chloramphenicol and gentamicin as antibacterial agents.Inoculum preparation. A suspension from 5-day precultures on a Sabouraud agar medium was calibrated with a hemacytometer at 106 spores per ml in sterile distilled water. Then, 2 ml was added to 18 ml of sterile Yeast Nitrogen Base with 2% glucose (YNBG) medium. The final inoculum was 105 spores per ml.Culture medium. Strain development was carried out in liquid YNBG medium containing Yeast Nitrogen Base (6.7 g; Difco, Detroit, Mich.), monohydrated glucose (20 g; Merck, Darmstadt, Germany), and distilled water to 1 liter. Aliquots of 18 ml were also prepared.Study of strain growth. A total of 900 ,ul of inoculated YNBG medium and 100 ,ul of sterile water were placed in sterile 1.5-ml microtubes (1.5-ml polypropylene microcentrifuge test tubes; Treff AG, Degershein, Switzerland). Incubation was performed at 25°C, and the glucose concentration in the medium was determined daily.Antifungal agents. An amphotericin B (graciously donated by Bristol-Myers Squibb, Paris, France) stock solution of 462 ug/ml was prepared by dissolving the powdered drug in a mixture containing dimethyl sulfoxide (Merck)-5% Tween 80 (Sigma, St. Louis, Mo.) in distilled wa...

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Section: Discussionmentioning

confidence: 99%

New in vitro assay based on glucose consumption for determining intraconazole and amphotericin B activities against Aspergillus fumigatus

Garrigues

Fontenay

Linas

et al. 1994

Antimicrob Agents Chemother

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Candidacell wall mannan in the vagina and its association with the signs and symptoms of vaginal candidosis

et al. 1991

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Effect of Pea Protein Plus Grape Seed Dry Extract on a Murine Model of Candida Albicans Induced Vaginitis

et al. 2018

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Aim: The objective of this research was to evaluate the antifungal properties of the association between grape seed and pea by using a nonpharmacological medical device that contains them. Materials & methods: A murine model of vulvovaginal candidiasis, induced by Candida albicans infection, was used. Results: We showed that topical treatment with the device significantly reduced the fungal burden in vagina and preserved vagina tissue architecture from C. albicans infection. Conclusion: We can support the potential beneficial effect of the association between grape and pea extract present in the medical device. Together these results supported this device as a favorable antifungal agent and a promising synergist with fluconazole in the clinical management of vulvovaginal candidiasis caused by C. albicans biofilms.

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Mannan estimation as a measure of the growth ofCandida albicans

Cited by 4 publications

References 20 publications

New in vitro assay based on glucose consumption for determining intraconazole and amphotericin B activities against Aspergillus fumigatus

New in vitro assay based on glucose consumption for determining intraconazole and amphotericin B activities against Aspergillus fumigatus

Candidacell wall mannan in the vagina and its association with the signs and symptoms of vaginal candidosis

Effect of Pea Protein Plus Grape Seed Dry Extract on a Murine Model of Candida Albicans Induced Vaginitis

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