Effect of Pea Protein Plus Grape Seed Dry Extract on a Murine Model of
            <i>Candida Albicans</i>
            Induced Vaginitis

Esposito, Emanuela; Campolo, Michela; Casili, Giovanna; Lanza, Marika; Filippone, Alessia; Peritore, Alessio Filippo; Cuzzocrea, Salvatore

doi:10.2217/fmb-2018-0102

Cited by 9 publications

(12 citation statements)

References 28 publications

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“…Thus concluding the probable benefit of the association between grape and pea extract that is present in the device. In all these results pointed to the device being helpful antifungal agent and helpful synergistic device along with fluconazole in the treatment of clinical vulvo vaginal candidiasis by candida albicans biofilms [50].…”

Section: Role Of Novel Agentsmentioning

confidence: 88%

Increasing Virulence of Vulvovaginal Candididiasis (VVC) in the Modern Era with Reducing Immunity by Different Candida Species Along with Increasing Resistance and Management -An Update

Kaur¹,

Allahbadia²,

Singh³

2019

Act Scie Micro

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Although Candida yeasts are present as vaginal microbiota, presence of them always doesn't lead to clinical symptoms. Interactions between host defense functions besides synergism with a variety of microorganisms from the vaginal mucosa are the ones which maintain balance of vaginal surroundings. On breakdown of the vaginal ecosystem, overgrowth of Candida yeasts is helped that results in development of vulvovaginal candidiasis (VVC). VVC is an opportunistic mucosal infection being 2 nd commonest vaginal infection that affects women in reproductive age, affecting 75% of these women of which 50% at least get recurrence of the same infection once. Recently globally it has been found candidiasis is becoming a threat especially for women with poor immunity specially those with HIV infection. Further not only the original conventional candida albicans is becoming responsible for the infection with candida but other species like C. tropicalis and C. parapilasis and C. gabratus ar becoming the pathogenic organisms involved in causing VVC not only in nonpregnant women but also in women with pregnancy where it is becoming a threat for the fetus as well.Here we review the role of various Lactobacillus species, their biosurfactant properties and role of moonlighting proteins in the virulence of candida, besides further measures to reduce the virulence and treatment of VVC effectively. Although Candida yeasts are present as vaginal microbiota, presence of them always doesn't lead to clinical symptoms. Interactions between host defense functions besides synergism with a variety of microorganisms from the vaginal mucosa are the ones which maintain balance of vaginal surroundings [1]. On breakdown of the vaginal ecosystem, overgrowth of Candida yeasts is helped that results in development of vulvovaginal candidiasis (VVC) [2]. VVC an opportunistic mucosal infection being 2nd commonest vaginal infection that affects women in reproductive age [3], affecting 75% of women once in a lifetime, and 50% of them suffering a minimum of a single recurrence [3,4]. Initial symptoms of VVC are pruritus and soreness of the vulva, dysuria, white cottage cheese like vaginal discharge along with dyspareunia. Quality of life gets affected by VVC besides predisposing to development of HIV susceptibility [5]. Though very little mortality is there with VVC symptoms =>marked morbidity mainly in women infected with human immunodeficiency virus (HIV) [6]. Moreover, women having vaginal colonization of Candida spp during 2nd trimester of Methods Thus we used the search engine PubMed using the Me SH terms like vulvovaginal candidiasis; candida species; lactobacillus species; drugs for treatment of VVC; Drug resistance; virulence; VVC in pregnancy; moonlighting proteins; probiotics; immunodeficiency and VVC. pregnancy have less neonatal birth weights and > preterm birth in contrast to colonization by other microbes [7]. Results and DiscussionWe found a total of 3846 articles out of which we selected 50 articles for this review. No meta-analysis was done. ...

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Section: Role Of Novel Agentsmentioning

confidence: 88%

Increasing Virulence of Vulvovaginal Candididiasis (VVC) in the Modern Era with Reducing Immunity by Different Candida Species Along with Increasing Resistance and Management -An Update

Kaur¹,

Allahbadia²,

Singh³

2019

Act Scie Micro

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“…In the current study, polymethacrylates demonstrated several properties as topical agents for VVC/RVVC, including high efficacy in killing epithelia-based C. albicans biofilms and persister cells, ease of formulation as a hydrogel for topical application, and low toxicity to local vaginal tissue. Unlike other newly proposed antifungal substances from natural sources that often need to be combined with conventional agents such as fluconazole or miconazole for high in vivo effectiveness (Gao et al, 2016; Esposito et al, 2018; Fernandes Costa et al, 2019), polymethacrylates provide a distinct advantage as they can be used as a sole therapy. They also outperformed clotrimazole and nystatin, two market leading antifungals, at 50 times less the equivalent clinical concentration in killing biotic C. albicans biofilms grown on vaginal epithelia.…”

Section: Discussionmentioning

confidence: 99%

RAFT-Derived Polymethacrylates as a Superior Treatment for Recurrent Vulvovaginal Candidiasis by Targeting Biotic Biofilms and Persister Cells

Zhang

et al. 2019

Front. Microbiol.

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BackgroundVulvovaginal candidiasis (VVC) is a common infection in need of more effective treatment. Formation of epithelium-associated Candida biofilms and the presence of persister cells are among the major contributing factors to the recurrence of this condition. We have previously developed RAFT-derived polymethacrylates that are effective in killing C. albicans biofilms in vitro. This study aimed to examine the clinical potential of polymethacrylates as antifungals for treatment of recurrent VVC (RVVC).MethodsA mouse model of VVC was used to establish vaginal epithelium-associated biofilms, using C. albicans isolates from VVC/RVVC patients. A comparison was made of the efficacies of polymethacrylates and conventional antifungals, clotrimazole and nystatin, in killing Candida in epithelium-associated biofilms in vivo. Ex vivo biofilms were used for Candida population profiling and to quantify persister cells in vaginal epithelia. The potency of polymethacrylates and conventional antifungals against persister cells, either as sole agents or in combination, was assessed.ResultsPolymethacrylates showed negligible local toxicity, resistance to vaginal acidity, and outstanding in vivo activity against vaginal epithelium-associated C. albicans biofilms. In vivo tests polymethacrylates outperformed the conventional antifungals, nystatin and clotrimazole at concentrations 50 times below the over-the-counter concentrations. Using polymethacrylates was associated with fewer persister cells, and better eradication of persister cells pre-selected by conventional antifungals.ConclusionThis study systematically assessed the clinical potential of RAFT-derived polymethacrylates as an effective treatment for VVC/RVVC in a mouse model. Polymethacrylates effectively killed vaginal epithelium-related C. albicans in vivo by specially targeting biotic biofilms and persister cells. Treatment presented negligible local toxicity.

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“…Pea protein (PP) is derived from the plant of Pisum sativum ; it is rich in fiber and represents an important source of essential amino acids such as lysine 33 . PP has shown to exert several health benefits including protective action on mucosal tissues 34 . Grape seed extract (GS) has gained great interest since it is rich in antioxidants and oligomeric proanthocyanidins 34 , while lactic acid (LA) is well-known for its ability to maintain the physiological vaginal pH and regulate the vaginal immune response 10 .…”

Section: Introductionmentioning

confidence: 99%

Effect of a combination of pea protein, grape seed extract and lactic acid in an in vivo model of bacterial vaginosis

Lanza

Scuderi²,

Capra³

et al. 2023

Sci Rep

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Bacterial vaginosis (BV) is a common vaginal dysbiosis characterized by a malodorous discharge and irritation. The imbalance of the vaginal microbiota plays a key role in the development of BV. It has been demonstrated that Gardnerella vaginalis (GV), a facultative anaerobic bacillus, is involved in BV. Due to the rising number of antimicrobial-resistant species, recurrence of BV is becoming more frequent in women; thus, alternative treatments to antibiotics are needed. Natural substances have recently shown a great efficacy for the treatment of vaginal dysbiosis. Thus, this study aimed to investigate the beneficial effect of a product containing pea protein (PP), grape seed extract (GS) and lactic acid (LA) in an in vivo model of Gardnerella vaginalis-induced vaginosis by intravaginal administration of GV suspension (1 × 106 CFU/20 µL saline). Our results demonstrated that the product containing PP, GS and LA significantly reduced GV proliferation. More specifically, it significantly preserved tissue architecture and reduced neutrophil infiltration, inflammatory markers and sialidase activity when used both as a pre- or a post-treatment. Moreover, the product displayed strong bioadhesive properties. Therefore, our data suggested that the product containing PP, GS and LA could be used as alternative preventive or curative treatment for the management of BV.

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Effect of Pea Protein Plus Grape Seed Dry Extract on a Murine Model of Candida Albicans Induced Vaginitis

Cited by 9 publications

References 28 publications

Increasing Virulence of Vulvovaginal Candididiasis (VVC) in the Modern Era with Reducing Immunity by Different Candida Species Along with Increasing Resistance and Management -An Update

Increasing Virulence of Vulvovaginal Candididiasis (VVC) in the Modern Era with Reducing Immunity by Different Candida Species Along with Increasing Resistance and Management -An Update

RAFT-Derived Polymethacrylates as a Superior Treatment for Recurrent Vulvovaginal Candidiasis by Targeting Biotic Biofilms and Persister Cells

Effect of a combination of pea protein, grape seed extract and lactic acid in an in vivo model of bacterial vaginosis

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