Manipulation of SlMXl for enhanced carotenoids accumulation and drought resistance in tomato

Ewas, Mohamed; Gao, Yongwen; Wang, Shouchuang; Liu, Xianqing; Zhang, Hongyan; Nishawy, Elsayed; Ali, Farhan; Shahzad, Raheel; Ziaf, Khurram; Subthain, Hizar; Martin, Cathie

doi:10.1007/s11434-016-1108-9

Cited by 55 publications

(64 citation statements)

References 11 publications

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“…Given the relationship between trichomes and water relations, recent research has evaluated the role of trichomes in tolerance and adaptation to water stress (Sletvold, 2011; Ewas et al , 2016; Mo et al , 2016; Galdon-Armero et al , 2018). In Arabidopsis lyrata , plants with higher density of trichomes were more tolerant to drought than glabrous plants (Sletvold, 2011).…”

Section: Discussionmentioning

confidence: 99%

TheLanatatrichome mutation increases stomatal conductance and reduces leaf temperature in tomato

Gasparini

Souto

Silva

et al. 2020

Preprint

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Background and aimsTrichomes are epidermal structures with an enormous variety of ecological functions and economic applications. Glandular trichomes produce a rich repertoire of secondary metabolites, whereas non-glandular trichomes create a physical barrier against biotic and abiotic stressors. Intense research is underway to understand trichome development and function and enable breeding of more resilient crops. However, little is known on how enhanced trichome density would impinge on leaf photosynthesis, gas exchange and energy balance.MethodsPrevious work has compared multiple species differing in trichome density, instead here we analyzed monogenic trichome mutants in a single tomato genetic background (cv. Micro-Tom). We determined growth parameters, leaf spectral properties, gas exchange and leaf temperature in the hairs absent (h), Lanata (Ln) and Woolly (Wo) trichome mutants.Key resultsShoot dry mass, leaf area, leaf spectral properties and cuticular conductance were not affected by the mutations. However, the Ln mutant showed increased carbon assimilation (A) possibly associated with higher stomatal conductance (gs), since there were no differences in stomatal density or stomatal index between genotypes. Leaf temperature was furthermore reduced in Ln in the early hours of the afternoon.ConclusionsWe show that a single monogenic mutation can increase glandular trichome density, a desirable trait for crop breeding, whilst concomitantly improving leaf gas exchange and reducing leaf temperature.HIGHLIGHTA monogenic mutation in tomato increases trichome density and optimizes gas exchange and leaf temperature

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Section: Discussionmentioning

confidence: 99%

TheLanatatrichome mutation increases stomatal conductance and reduces leaf temperature in tomato

Gasparini

Souto

Silva

et al. 2020

Preprint

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“…In Arabidopsis lyrata, trichome production has been linked to improved performance under drought conditions (Sletvold and Agren, 2012). In tomato, SlMX1-overexpressing plants with high trichome density showed a higher tolerance to water stress compared with unmodified plants (Ewas et al, 2016). In Citrullus lanatus (watermelon), wild, drought-tolerant genotypes have increased trichome density compared with domesticated, drought-sensitive varieties (Mo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The ratio of trichomes to stomata is associated with water use efficiency in Solanum lycopersicum (tomato)

et al. 2018

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Trichomes are specialised structures that originate from the aerial epidermis of plants, and play key roles in the interaction between the plant and the environment. In this study we investigated the trichome phenotypes of four lines selected from the Solanum lycopersicum × Solanum pennellii introgression line (IL) population for differences in trichome density, and their impact on plant performance under water-deficit conditions. We performed comparative analyses at morphological and photosynthetic levels of plants grown under well-watered (WW) and also under water-deficit (WD) conditions in the field. Under WD conditions, we observed higher trichome density in ILs 11-3 and 4-1, and lower stomatal size in IL 4-1 compared with plants grown under WW conditions. The intrinsic water use efficiency (WUE ) was higher under WD conditions in IL 11-3, and the plant-level water use efficiency (WUE ) was also higher in IL 11-3 and in M82 for WD plants. The ratio of trichomes to stomata (T/S) was positively correlated with WUE and WUE , indicating an important role for both trichomes and stomata in drought tolerance in tomato, and offering a promising way to select for improved water use efficiency of major crops.

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“…In cotton, the MIXTA‐like transcription factor GhMYB25 (GbMYB25) and HD‐ZIP IV gene GhHD1 (GbML1) were proven to interact with each other, and both of them have a positive effect on regulating trichome initiation in seed (fiber) and other parts of the plant, as well as fiber elongation (Machado et al ., ; Zhang et al ., ; Walford et al ., ). The tomato SlMIXTA‐like gene was reported to regulate cuticle biosynthesis and leaf trichome formation (Lashbrooke et al ., ; Ewas et al ., ), and the HD‐ZIP IV gene SlCD2 was also shown to regulate cuticle metabolism and leaf trichome density (Nadakuduti et al ., ). Furthermore, promoter analysis of downregulated genes in SlMIXTA‐RNAi lines showed a significant enrichment of the L1‐box (Lashbrooke et al ., ), which indicates a coregulation network existing between SlMIXTA‐like and the HD‐ZIP IV transcription factors (possibly SlCD2) through interaction.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, is there a connection between the regulatory networks of two processes? Mutants or transgenic plants of several transcription factors, including SHN1 , SlMIXTA‐like and SlCD2 , exhibit altered cuticle components and reduced trichome numbers simultaneously (Aharoni et al ., ; Nadakuduti et al ., ; Lashbrooke et al ., ; Ewas et al ., ), suggesting that the two processes may involve an overlapping molecular basis. Similarly, in the present study, we found that the AaHD8/AaMIXTA1 complex regulated trichome formation as well as cuticle development, indicating that the regulatory network of the two processes partially overlapped in A. annua .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, they usually have similar functions. In Arabidopsis, they promote cuticle biosynthesis (AtMYB16, Oshima et al ., ; AtMYB106, Oshima et al ., ; AtHDG1, Wu et al ., ; and AtANL2, Nadakuduti et al ., ) and negatively regulate trichome branching (AtMYB106, Jakoby et al ., ; AtHDG11, Nakamura et al ., ; and AtHDG12, Nakamura et al ., ); in cotton they positively regulate fiber initiation and elongation (GhMYB25, Machado et al ., ; GhMYB25‐like, Walford et al ., ; GhHOX3, Shan et al ., ; and GhHD1, Walford et al ., ); and in tomato, they are the positive regulators of cuticle and trichome development (SlMIXTA‐like, Lashbrooke et al ., ; Ewas et al ., ; and SlCD2, Nadakuduti et al ., ). Nevertheless, the relationship between HD‐ZIP IV and MIXTA‐like transcription factors in regulating epidermal development is still largely unknown.…”

Section: Introductionmentioning

confidence: 97%

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A novel HD‐ZIP IV/MIXTA complex promotes glandular trichome initiation and cuticle development in Artemisia annua

et al. 2018

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Glandular trichomes and cuticles are both specialized structures that cover the epidermis of aerial plant organs. The former are commonly regarded as 'biofactories' for producing valuable natural products. The latter are generally considered as natural barriers for defending plants against abiotic and biotic stresses. However, the regulatory network for their formation and relationship remains largely elusive. Here we identify a homeodomain-leucine zipper (HD-ZIP) IV transcription factor, AaHD8, directly promoting the expression of AaHD1 for glandular trichome initiation in Artemisia annua. We found that AaHD8 positively regulated leaf cuticle development in A. annua via controlling the expression of cuticle-related enzyme genes. Furthermore, AaHD8 interacted with a MIXTA-like protein AaMIXTA1, a positive regulator of trichome initiation and cuticle development, forming a regulatory complex and leading to enhanced transcriptional activity in regulating the expression of AaHD1 and cuticle development genes. Our results reveal a molecular mechanism by which a novel HD-ZIP IV/MIXTA complex plays a significant role in regulating epidermal development, including glandular trichome initiation and cuticle formation.

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Manipulation of SlMXl for enhanced carotenoids accumulation and drought resistance in tomato

Cited by 55 publications

References 11 publications

TheLanatatrichome mutation increases stomatal conductance and reduces leaf temperature in tomato

TheLanatatrichome mutation increases stomatal conductance and reduces leaf temperature in tomato

The ratio of trichomes to stomata is associated with water use efficiency in Solanum lycopersicum (tomato)

A novel HD‐ZIP IV/MIXTA complex promotes glandular trichome initiation and cuticle development in Artemisia annua

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