Manipulation of culture conditions alters lipid content and fatty acid profiles of a wide variety of known and new oleaginous yeast species

Abstract

Oleaginous yeasts have been studied for oleochemical production for over 80 years. Only a few species have been studied intensely. To expand the diversity of oleaginous yeasts available for lipid research, we surveyed a broad diversity of yeasts with indicators of oleaginicity including known oleaginous clades, and buoyancy. Sixty-nine strains representing 17 genera and 50 species were screened for lipid production. Yeasts belonged to Ascomycota families, Basidiomycota orders, and the yeast-like algal genus Prototheca. Total intracellular lipids and fatty acid composition were determined under different incubation times and nitrogen availability. Thirteen new oleaginous yeast species were discovered, representing multiple ascomycete and basidiomycete clades. Nitrogen starvation generally increased intracellular lipid content. The fatty acid profiles varied with the growth conditions regardless of taxonomic affiliation. The dominant fatty acids were oleic acid, palmitic acid, linoleic acid, and stearic acid. Yeasts and culture conditions that produced fatty acids appropriate for biodiesel were identified.

Acetate-detoxification of wood hydrolysates with alkali tolerant Bacillus sp. as a strategy to enhance the lipid production from Rhodosporidium toruloides

“…Oleic acid in particular is considered as beneficial during the subsequent production of biodiesel (Sitepu et al, 2013). The fatty acid composition was similar to the compositions obtained previously with the same oleaginous yeast Wang et al, 2012).…”

New yeast-based approaches in production of palmitoleic acid

“…As stated by Sitepu et al (2013), relative FA composition varies depending on the composition of the medium and cultivation time.…”

“…These data correspond to published results . The major FA was oleic acid, whose proportion was within the range of 32-60% of total FA and it could therefore be used for biotechnological production for nutritional use (Sitepu et al, 2013). The conditions best suited for its production were at C/N of 30, ideally even with a higher C/P ratio.…”

Lipid production in the under-characterized oleaginous yeasts, Rhodosporidium babjevae and Rhodosporidium diobovatum, from biodiesel-derived waste glycerol

“…As such, in terms of producing high amounts of SCO without 'losing' available carbon to side-products, it could be advantageous to use alternate oleaginous yeast species. Recently, Sitepu et al (2013) reported the lipid production potential of a wide variety of oleaginous yeasts strains spanning several genera when grown using pure dextrose as the carbon source. Two of the highest lipid producers identified by this study were Rhodosporidium babjevae 05-775 and Rhodosporidium diobovatum 08-225.…”