Manipulating the mouse embryo: A laboratory manual

Johnson, Martin

doi:10.1016/0168-9525(86)90275-1

Cited by 19 publications

(33 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One female and one male pup were randomly selected per E18.5 litter for skeletal staining analysis. Skeletal staining was performed according to the Hogan et al method (Johnson et al, 1986). Briefly, E18.5 carcasses were skinned and eviscerated before overnight fixation in 95% ethanol (EtOH).…”

Section: Skeletal Stainingmentioning

confidence: 99%

Histone H3 lysine 4 trimethylation in sperm is transmitted to the embryo and associated with diet-induced phenotypes in the offspring

et al. 2021

View full text Add to dashboard Cite

Section: Skeletal Stainingmentioning

confidence: 99%

Histone H3 lysine 4 trimethylation in sperm is transmitted to the embryo and associated with diet-induced phenotypes in the offspring

et al. 2021

View full text Add to dashboard Cite

“…Constitutive Fhod1 knockout mice were generated by injection of gene-trapped embryonic stem cells into blastocyst stage embryos derived from super-ovulated female mice using methods as previously described [ 18 ]. Gene trapped ES cells for Fhod1 (IST13553G8) were obtained from the Texas Institute for Genomic Medicine ( https://www.tigm.org ).…”

Section: Methodsmentioning

confidence: 99%

“…However, platelet function is unaffected by the loss of these two proteins. previously described [18]. Gene trapped ES cells for Fhod1 (IST13553G8) were obtained from the Texas Institute for Genomic Medicine (https://www.tigm.org).…”

Section: Introductionmentioning

confidence: 99%

Loss of mDia1 and Fhod1 impacts platelet formation but not platelet function

et al. 2020

View full text Add to dashboard Cite

An organized and dynamic cytoskeleton is required for platelet formation and function. Formins are a large family of actin regulatory proteins which are also able to regulate microtubule dynamics. There are four formin family members expressed in human and mouse megakaryocytes and platelets. We have previously shown that the actin polymerization activity of formin proteins is required for cytoskeletal dynamics and platelet spreading using a small molecule inhibitor. In the current study, we analyze transgenic mouse models deficient in two of these proteins, mDia1 and Fhod1, along with a model lacking both proteins. We demonstrate that double knockout mice display macrothrombocytopenia which is due to aberrant megakaryocyte function and a small decrease in platelet lifespan. Platelet function is unaffected by the loss of these proteins. This data indicates a critical role for formins in platelet and megakaryocyte function.

show abstract

“…One of the six TS23 embryos was scanned twice and the obtained images were registered to evaluate the precision of the deformable registration algorithm used to measure the developmental map [20]. The hindlimbs were stained with 0.015% Alcian Blue for cartilage [23] and processed for scanning with Optical Projection Tomography (OPT) [21] as reported in detail in [24]. OPT [25] constructs an image by measuring the amount of light transmitted or emitted by an object when light is shone upon it.…”

Section: Methodsmentioning

confidence: 99%

Prenatal growth map of the mouse knee joint by means of deformable registration technique

et al. 2019

View full text Add to dashboard Cite

Joint morphogenesis is the process during which distinct and functional joint shapes emerge during pre- and post-natal joint development. In this study, a repeatable semi-automatic protocol capable of providing a 3D realistic developmental map of the prenatal mouse knee joint was designed by combining Optical Projection Tomography imaging (OPT) and a deformable registration algorithm (Sheffield Image Registration toolkit, ShIRT). Eleven left limbs of healthy murine embryos were scanned with OPT (voxel size: 14.63μm) at two different stages of development: Theiler stage (TS) 23 (approximately 14.5 embryonic days) and 24 (approximately 15.5 embryonic days). One TS23 limb was used to evaluate the precision of the displacement predictions for this specific case. The remaining limbs were then used to estimate Developmental Tibia and Femur Maps. Acceptable uncertainties of the displacement predictions computed from repeated images were found for both epiphyses (between 1.3μm and 1.4μm for the proximal tibia and between 0.7μm and 1.0μm for the femur, along all directions). The protocol was found to be reproducible with maximum Modified Housdorff Distance (MHD) differences equal to 1.9 μm and 1.5 μm for the tibial and femoral epiphyses respectively. The effect of the initial shape of the rudiment affected the developmental maps with MHD of 21.7 μm and 21.9 μm for the tibial and femoral epiphyses respectively, which correspond to 1.4 and 1.5 times the voxel size. To conclude, this study proposes a repeatable semi-automatic protocol capable of providing mean 3D realistic developmental map of a developing rudiment allowing researchers to study how growth and adaptation are directed by biological and mechanobiological factors.

show abstract

Manipulating the mouse embryo: A laboratory manual

Cited by 19 publications

References 0 publications

Histone H3 lysine 4 trimethylation in sperm is transmitted to the embryo and associated with diet-induced phenotypes in the offspring

Histone H3 lysine 4 trimethylation in sperm is transmitted to the embryo and associated with diet-induced phenotypes in the offspring

Loss of mDia1 and Fhod1 impacts platelet formation but not platelet function

Prenatal growth map of the mouse knee joint by means of deformable registration technique

Contact Info

Product

Resources

About