Histone H3 lysine 4 trimethylation in sperm is transmitted to the embryo and associated with diet-induced phenotypes in the offspring

Lismer, Ariane; Dumeaux, Vanessa; Lafleur, Christine; Lambrot, Romain; Brind’Amour, Julie; Lorincz, Matthew C.; Kimmins, Sarah

doi:10.1016/j.devcel.2021.01.014

Cited by 78 publications

(125 citation statements)

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“… Timing: 2.5–4 months Expose male mice to an environmental stressor of interest (e.g., toxicant, diet, trauma…) for two full spermatogenic cycles beginning at weaning (i.e., 3 weeks of age). Alternatives: Timing and duration of environmental exposure can vary as follows: Exposure may begin in utero by exposing a pregnant female to the environmental exposure ( Lambrot et al., 2013 ), at weaning during the first wave of spermatogenesis ( Lismer et al., 2021 ), or at adulthood. Exposure should last at least one full spermatogenic cycle (35 days in mice) ( Oakberg, 1956 ) but can extend throughout multiple cycles.…”

Section: Before You Beginmentioning

confidence: 99%

“… Exposure should last at least one full spermatogenic cycle (35 days in mice) ( Oakberg, 1956 ) but can extend throughout multiple cycles. CRITICAL: In Lismer et al., 2021 , folate sufficient (FS) male mice were fed a diet with 2.0 mg of folate/kg, corresponding to the recommended folate amount for rodents ( Reeves et al., 1993 ). Folate deficient (FD) mice were given a diet composed of 0.3 mg of folate/kg, consistent with the folate intake measured in folate deficient populations ( Rogers et al., 2018 ).…”

Section: Before You Beginmentioning

confidence: 99%

“…Exposure may begin in utero by exposing a pregnant female to the environmental exposure ( Lambrot et al., 2013 ), at weaning during the first wave of spermatogenesis ( Lismer et al., 2021 ), or at adulthood.…”

Section: Before You Beginmentioning

confidence: 99%

“…We place emphasis on how to obtain high-quality and quantifiable data sets in sperm and embryos, as well as highlight the limitations of working with low input. For complete details on the use and execution of this protocol, please refer to Lismer et al. (2021) .…”

mentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Lismer et al. (2021) .…”

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confidence: 99%

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ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance

et al. 2021

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Summary In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We describe mouse model design to probe chromatin states in single mouse sperm and techniques to assess pre-implantation embryo chromatin and gene expression. We place emphasis on how to obtain high-quality and quantifiable data sets in sperm and embryos, as well as highlight the limitations of working with low input. For complete details on the use and execution of this protocol, please refer to Lismer et al. (2021) .

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Section: Before You Beginmentioning

confidence: 99%

Section: Before You Beginmentioning

confidence: 99%

Section: Before You Beginmentioning

confidence: 99%

mentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Lismer et al. (2021) .…”

mentioning

confidence: 99%

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ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance

et al. 2021

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Teratogenesis and the epigenetic programming of congenital defects: Why paternal exposures matter

Golding

2023

Birth Defects Research

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Until recently, clinicians and researchers did not realize paternal exposures could impact child developmental outcomes. Indeed, although there is growing recognition that sperm carry a large amount of non‐genomic information and that paternal stressors influence the health of the next generation, toxicologists are only now beginning to explore the role paternal exposures have in dysgenesis and the incidence of congenital malformations. In this commentary, I will briefly summarize the few studies describing congenital malformations resulting from preconception paternal stressors, argue for the theoretical expansion of teratogenic perspectives into the male preconception period, and discuss some of the challenges in this newly emerging branch of toxicology. I argue that we must consider gametes the same as any other malleable precursor cell type and recognize that environmentally‐induced epigenetic changes acquired during the formation of the sperm and oocyte hold equal teratogenic potential as exposures during early development. Here, I propose the term epiteratogen to reference agents acting outside of pregnancy that, through epigenetic mechanisms, induce congenital malformations. Understanding the interactions between the environment, the essential epigenetic processes intrinsic to spermatogenesis, and their cumulative influences on embryo patterning is essential to addressing a significant blind spot in the field of developmental toxicology.

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Bridging the explanatory gaps: What can we learn from a biological agency perspective?

2021

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We begin this article by delineating the explanatory gaps left by prevailing gene‐focused approaches in our understanding of phenotype determination, inheritance, and the origin of novel traits. We aim not to diminish the value of these approaches but to highlight where their implementation, despite best efforts, has encountered persistent limitations. We then discuss how each of these explanatory gaps can be addressed by expanding research foci to take into account biological agency—the capacity of living systems at various levels to participate in their own development, maintenance, and function by regulating their structures and activities in response to conditions they encounter. Here we aim to define formally what agency and agents are and—just as importantly—what they are not, emphasizing that agency is an empirical property connoting neither intention nor consciousness. Lastly, we discuss how incorporating agency helps to bridge explanatory gaps left by conventional approaches, highlight scientific fields in which implicit agency approaches are already proving valuable, and assess the opportunities and challenges of more systematically incorporating biological agency into research programs.

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Histone H3 lysine 4 trimethylation in sperm is transmitted to the embryo and associated with diet-induced phenotypes in the offspring

Cited by 78 publications

References 74 publications

ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance

ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance

Teratogenesis and the epigenetic programming of congenital defects: Why paternal exposures matter

Bridging the explanatory gaps: What can we learn from a biological agency perspective?

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