Ceratocystis wilt, caused by Ceratocystis fimbriata, is one of the most damaging diseases in eucalyptus plantations worldwide. Although there are resistant genotypes, the genetic basis of resistance is still poorly understood. In this paper we studied the resistance level by a stem inoculation experiment of genotypes of Eucalyptus grandis and E. urophylla and estimated the heritability and gains of selection in families derived from controlled interspecific crosses. In both species, highly resistant as well as highly susceptible genotypes to Ceratocystis wilt were found. Out of 21 parents assessed, twelve were resistant and nine susceptible. Estimates of individual narrow (50 %) and broad (59 %) sense heritability suggested a high degree of genetic control and low allelic dominance of the trait. There was great genetic variation among and within families, a fact that contributes to high heritability and genetic gain. A genetic gain in lesion size of up to -74.4 % was obtained from selection of the 50 best clones in the evaluated families, i.e., the mean lesion length in the progeny population can be reduced by 74,4 %.