Mango Fruit Extracts Differentially Affect Proliferation and Intracellular Calcium Signalling in MCF-7 Human Breast Cancer Cells

Abstract

The assessment of human cancer cell proliferation is a common approach in identifying plant extracts that have potential bioactive effects. In this study, we tested the hypothesis that methanolic extracts of peel and flesh from three archetypal mango cultivars, Irwin (IW), Nam Doc Mai (NDM), and Kensington Pride (KP), differentially affect proliferation, extracellular signal-regulated kinase (ERK) activity, and intracellular calcium ([Ca2+]I) signalling in MCF-7 human breast cancer cells. Mango flesh extracts from all three cultivars did not inhibit cell growth, and of the peel extracts only NDM reduced MCF-7 cell proliferation. Mango cultivar peel and flesh extracts did not significantly change ERK phosphorylation compared to controls; however, some reduced relative maximal peak[Ca2+]Iafter adenosine triphosphate stimulation, with NDM peel extract having the greatest effect among the treatments. Our results identify mango interfruit and intrafruit (peel and flesh) extract variability in antiproliferative effects and[Ca2+]Isignalling in MCF-7 breast cancer cells and highlight that parts of the fruit (such as peel and flesh) and cultivar differences are important factors to consider when assessing potential chemopreventive bioactive compounds in plants extracts.

Antioxidant (gallic acid and quercetin) profile of Sumatran wild mangoes (Mangifera spp.): a potential source for antidegenerative medicine

“…They may even be used to manage chronic diseases, such as cancer. In the study by Taing et al 5 , skin and flesh extracts of mangos (M. indica) had the remarkable action of inhibiting proliferation of breast and colon cancer cells 6 .…”

Radical scavenging and anti‐proliferative capacity of three freeze‐dried tropical fruits

“…The antiproliferative capacity of polar phytochemicals from several mango varieties on diverse cell lines such as breast (MDA‐MB‐231, BT474, MCF‐7), liver (HepG2), lung (A‐549), prostate (LnCap) and colon (SW‐480) cancer cells, leukaemia (HL‐60, Molt‐4) and normal colon cells (CCD‐18Co), has been reported so far (García‐Solís et al ., ; Kim et al ., ; Noratto et al ., ; Luo et al ., ; Banerjee et al ., ; Taing et al ., ). Here, the antiproliferative capacity of mango was RAW264.7 > L929 > HeLa cell lines, with an IC 50 (μg mL −1 ) ranging from 100.7 to 151.4 μg mL −1 .…”

“…are natural functional foods. They have a vast array of extractable phytochemicals with radical scavenging and antiproliferative capacity that, if eaten in sufficient amounts, may help to prevent breast, ovarian, colon, lung, leukaemia and prostate (García‐Solís et al ., ; Kim et al ., ; Noratto et al ., ; Nguyen et al ., ; Luo et al ., ; Romano et al ., ; Sancho et al ., ; Banerjee et al ., ; Gani et al ., ; Roleira et al ., ; Taing et al ., ) cancers. However, tropical fruits have usually a very short shelf life, which limits their distribution and commercialisation.…”

“…Moreover, the sensitivity of normal and cancer cell lines is different upon the chemical nature of the specific profile of phytochemicals of a particular fruit variety, as it has been demonstrated in breast cancer cell line MCF‐7 with mango cv. Ataulfo (García‐Solís et al ., ), Irwin (IW), Nam Doc Mai and Kensington Pride (Taing et al ., ).…”

Morphological Characterization, Quality, Yield and DNA Fingerprinting of Biofield Energy Treated Alphonso Mango (<i>Mangifera indica</i> L.)

“…It also protects serum oxidative stress in senile people due to its antioxidant properties [6]. Antiproliferative activity of mangoes inter and intra fruits (peel and flesh) has been reported on breast cancer cell line [7]. Banerjee et al reported that due to presence of polyphenolics component it suppressed tumor growth in breast cancer xenografts in the mice model [8].…”