Mangiferin ameliorates fatty liver via modulation of autophagy and inflammation in high-fat-diet induced mice

Wang, Hu; Zhu, Yangyang; Wang, Li; Teng, Tian; Zhou, Meng; Wang, Shuguang; Tian, Yizhu; Du, Lei; Yin, Xiaoxing; Sun, Ying

doi:10.1016/j.biopha.2017.10.022

Cited by 52 publications

(36 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…, Wang et al . ), but not supported by the present observation that p62 protein was unchanged with HFF diet. Nevertheless, the lack of change in hepatic p62 protein is in accordance with previous mouse studies using sucrose supplement (Alex et al .…”

Section: Discussioncontrasting

confidence: 97%

See 1 more Smart Citation

Impact of liver PGC-1α on exercise and exercise training-induced regulation of hepatic autophagy and mitophagy in mice on HFF

et al. 2018

View full text Add to dashboard Cite

Hepatic autophagy has been shown to be regulated by acute exercise and exercise training. Moreover, high‐fat diet‐induced steatosis has been reported to be associated with impaired hepatic autophagy. In addition, autophagy has been shown to be regulated by acute exercise and exercise training in a PGC‐1α dependent manner in skeletal muscle. The aim of this study was to test the hypotheses that high‐fat high‐fructose (HFF) diet changes hepatic autophagy and mitophagy, that exercise training can restore this through a PGC‐1α‐mediated mechanism, and that acute exercise regulates autophagy and mitophagy in the liver. Liver samples were obtained from liver‐specific PGC‐1α KO mice and their littermate Lox/Lox mice fed a HFF diet or a control diet for 13 weeks. The HFF mice were either exercise trained (ExT) on a treadmill the final 5 weeks or remained sedentary (UT). In addition, half of each group performed at the end of the intervention an acute 1 h exercise bout. HFF resulted in increased hepatic BNIP3 dimer and Parkin protein, while exercise training increased BNIP3 total protein without affecting the elevated BNIP3 dimer protein. In addition, exercise training reversed a HFF‐induced increase in hepatic LC3II/LC3I protein ratio, as well as a decreased PGC‐1α mRNA level. Acute exercise increased hepatic PGC‐1α mRNA in HFF UT mice only. In conclusion, this indicates that exercise training in part reverses a HFF‐induced increase in hepatic autophagy and capacity for mitophagy in a PGC‐1α‐independent manner. Moreover, HFF may blunt acute exercise‐induced regulation of hepatic autophagy.

show abstract

Section: Discussioncontrasting

confidence: 97%

“…, Wang et al . ) as well as increased hepatic p62 protein (Ghareghani et al . , Gonzalez‐Rodriguez et al .…”

Section: Introductionmentioning

confidence: 99%

Impact of liver PGC-1α on exercise and exercise training-induced regulation of hepatic autophagy and mitophagy in mice on HFF

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The flavonoid quercetin modulated the hepatic metabolism of cholesterol that yields bile acids in Wistar rats by upregulating the LXRα mRNA and protein levels (Zhang et al, 2016). These and similar findings have made it possible to suggest that some polyphenols like gallic acid (Chao et al, 2014), mangiferin (Wang et al, 2017), and chlorogenic acid (Wan et al, 2013) may be suitable options to prevent, mitigate, or treat NAFLD.…”

Section: Discussionmentioning

confidence: 79%

Fiber and phenolic compounds contribution to the hepatoprotective effects of mango diets in rats fed high cholesterol/sodium cholate

Canizales

Domínguez-Ávila

Wall‐Medrano

et al. 2019

Phytotherapy Research

View full text Add to dashboard Cite

The present study evaluated the contribution of mango fiber (MF) and mango phenolic compounds (MP) to the hepatoprotective effect of freeze‐dried mango pulp (FDM) cultivar (cv.) “Ataulfo” diets in high cholesterol/sodium cholate (HCC)–fed rats. Male Wistar rats were fed with a HCC diet for 12 weeks, either untreated, or supplemented with MF, MP, FDM, or a control diet (no HCC; n = 6/group). All mango treatments significantly decreased hepatic cholesterol deposition and altered its fatty acid profile, whereas MF and MP mitigated adipose tissue hypertrophy. MF caused a lower level of proinflammatory cytokines (IL‐1α/β, IFN‐γ, TNF‐α) whereas FDM increased the anti‐inflammatory ones (IL‐4, 6, 10). Mango treatments increased catalase (CAT) activity and its mRNA expression; superoxide dismutase (SOD) activity was normalized by MF and FDM, but its activity was unrelated to its hepatic mRNA expression. Changes in CAT and SOD mRNA expression were unrelated to altered Nrf2 mRNA expression. Higher hepatic PPARα and LXRα mRNA levels were found in MP and MF. We concluded that MF and MP are highly bioactive, according to the documented hepatoprotection in HCC‐fed rats; their mechanism of action appears to be related to modulating cholesterol and fatty acid metabolism as well as to stimulating the endogenous antioxidant system.

show abstract

“…Thus, mangiferin may protect the lung damage against fibrosis by inhibiting the expression of pro‐inflammatory cytokines. In the previous study, many reports have shown that the anti‐inflammatory effect of mangiferin is through NF‐kB and MAPK pathway . However, whether the upstream signalling of NF‐kB and MAPK pathway is also the possible target of mangiferin is still unknown.…”

Section: Resultsmentioning

confidence: 99%

“…The oral LD 50 value of mangiferin in mice was 400 mg/kg, and this compound showed no cytotoxicity in cell culture when the concentration is as high as 100 m m . The anti‐inflammatory capacity of this compound has been discussed in many literatures . However, until now, the antifibrosis effect of this compound has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Mangiferin attenuates bleomycin-induced pulmonary fibrosis in mice through inhibiting TLR4/p65 and TGF-β1/Smad2/3 pathway

Park

Gao

et al. 2019

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

Objectives Investigating the antipulmonary fibrosis effect of mangiferin from Mangifera indica and the possible molecular mechanism. Methods In vivo, bleomycin (BLM)‐induced pulmonary fibrosis experimental model was used for evaluating antipulmonary fibrosis effect of mangiferin. Histopathologic examination and collagen deposition were investigated by HE and Masson staining as well as detecting the content of hydroxyproline. The expression of transforming growth factor‐β1 (TGF‐β1), α‐smooth muscle actin (α‐SMA), TLR4 and p‐P65 in lung tissue was analysed through immunofluorescence. Leucocytes and inflammatory cytokines including IL‐1β, IL‐6, TNF‐α and MCP‐1 in bronchoalveolar lavage fluid were detected by cell counting and enzyme‐linked immunosorbent assay. In vitro, TGF‐β1‐induced A549 epithelial–mesenchymal transition (EMT) cell model was used for investigating the possible molecular mechanism. Reactive oxygen species (ROS) generation was detected by DCFH‐DA assay. Expression of all proteins was examined by Western blot. Key findings Oral administration of mangiferin could attenuate the severity of BLM‐induced pulmonary fibrosis through increasing the survival rate, improving histopathological lesion and body weight loss as well as decreasing pulmonary index visibly. Pulmonary hydroxyproline content, TGF‐β1, and α‐SMA levels were reduced significantly. The molecular mechanism of mangiferin for inhibiting pulmonary fibrosis is that it could obviously inhibit the occurrence of inflammation and the secretion of inflammatory cytokine through inhibiting activation of TLR4 and phosphorylation of p65. Meanwhile, EMT process was suppressed obviously by mangiferin through blocking the phosphorylation of Smad2/3 and reducing MMP‐9 expression. Besides, mangiferin could significantly inhibit the process of oxidant stress through downregulating the intracellular ROS generation. Conclusions Mangiferin attenuates BLM‐induced pulmonary fibrosis in mice through inhibiting TLR4/p65 and TGF‐β1/Smad2/3 pathway.

show abstract

Mangiferin ameliorates fatty liver via modulation of autophagy and inflammation in high-fat-diet induced mice

Cited by 52 publications

References 36 publications

Impact of liver PGC-1α on exercise and exercise training-induced regulation of hepatic autophagy and mitophagy in mice on HFF

Impact of liver PGC-1α on exercise and exercise training-induced regulation of hepatic autophagy and mitophagy in mice on HFF

Fiber and phenolic compounds contribution to the hepatoprotective effects of mango diets in rats fed high cholesterol/sodium cholate

Mangiferin attenuates bleomycin-induced pulmonary fibrosis in mice through inhibiting TLR4/p65 and TGF-β1/Smad2/3 pathway

Contact Info

Product

Resources

About