Manganoxydierende Bakterien. I. Isolierung und Bestimmung einiger Stämme von Manganbakterien

Twenty years of experience with corrosion failures caused by manganese oxidizing microorganisms

“…Spot testing with benzidine is very simple, very sensitive and considered useful in particular for investigations on site. For the test, a small sample of deposit is transferred to filter paper and wetted with a drop of reagent (5 mg benzidine acetate in 10 mL of 10% acetic acid) 23. A blue colour reaction indicates a strong oxidant.…”

“…In the context of MIC by MOMOs of SS in hydroelectric power plants, the possible impact of MOMOs on bronze as an alternative material for turbines was investigated. Laboratory experiments with samples from SS and from aluminium bronze exposed to a nutrient medium (Schweisfurth's medium 14a, without agar 23) inoculated with original river water demonstrated the effect of ennoblement for SS 2, but did not exhibit a significant impact on the E OC of bronze. Since copper ions are known to be toxic to many, although not all, microorganisms, it was assumed that the formation of biofilm with MOMOs was suppressed on bronze.…”

“…Since copper ions are known to be toxic to many, although not all, microorganisms, it was assumed that the formation of biofilm with MOMOs was suppressed on bronze. This was tested by cultivating MOMOs in Petri dishes with Schweisfurth´s medium 14a with agar 23. A series of tests was run with increasing concentrations of copper ions (0, 0.1, 1 and 10 mg/L), added as copper sulphate to the medium.…”

The Molecular Geomicrobiology of Bacterial Manganese(II) Oxidation

“…The bulk of the genetic experiments investigating bacterial Mn(II) oxidation have been performed in the g-proteobacterium Pseudomonas putida GB-1 (and the closely related strain MnB1; (Schweisfurth 1973;Brouwers et al 1997;Caspi et al 1998), due to its genetic tractability and the ease with which it can be grown and maintained in the lab environment. Like the other characterized bacterial Mn(II) oxidizers, P. putida GB-1 precipitates Mn(IV) oxides on its cell surface (Okazaki et al 1997).…”

c -Type Cytochromes and Manganese Oxidation in Pseudomonas putida MnB1

“…With the isolation of more Mn-oxidizing bacteria over the years, it became clear that rather than being limited to a few specialized strains, Mn oxidation is widespread and is a common trait in ubiquitous seawater and freshwater pseudomonads (13,23,38). Pseudomonas manganoxidans MnB1 (ϭ ATCC 23483) was isolated from an Mn crust that accumulated in drinking water pipes in Trier, Germany (44), and was recently reclassified as a Pseudomonas putida strain (12), a fact that was confirmed by us on the basis of the sequence of the 16S rRNA gene. Upon reaching the stationary phase, this organism oxidizes Mn(II) in liquid and solid media to Mn(IV) oxyhydroxides, which are precipitated on the cell surface.…”