Manganese peroxidases of the white rot fungus Phanerochaete sordida

Rüttimann-Johnson, Carmen; Cullen, Daniel; Lamar, Richard T.

doi:10.1128/aem.60.2.599-605.1994

Cited by 100 publications

(34 citation statements)

References 43 publications

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“…Such an azide concentration should result in complete inhibition of laccase (Bollag and Leonowicz 1984). Furthermore, after dialysis of the culture ®ltrate (cut off 10 000 Da), ABTS oxidation was not detected in the retentate, which is in accordance with literature data (Ru Èttimann-Johnson et al 1994). As the molecular weight of laccase is over 60 kDa (Thurston 1994;Yaropolov et al 1994), it should have been retained and its activity preserved.…”

Section: Resultssupporting

confidence: 82%

“…Once the possibility that ABTS is oxidized by MnP or laccase had been excluded, the only reasonable oxidation agent remained the Mn(III) complex, which could be formed in the growth medium (Glenn et al 1986;Wariishi et al 1989). It is known that the complex oxidizes ABTS and syringaldazine (Ru Èttimann-Johnson et al 1994). This renders the conclusions of , Rodriguez Couto et al (1998) and Couto et al (1999) that P. chrysosporium produces laccase, questionable.…”

Section: Resultsmentioning

confidence: 98%

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Laccase production by Phanerochaete chrysosporium - an artefact caused by Mn(III)?

Podgornik¹,

Stegu²,

Zibert³

et al. 2001

Lett Appl Microbiol

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Aims: The possibility of laccase production by Phanerochaete chrysosporium was studied. Methods and Results: A relatively high initial Mn(II) concentration (1±4 mM) in the growth medium leads to the development of reddish-brown coloration and intensive oxidation of 2.2¢-azino-bis(3-etilbenz-tiazolin-6-sulfonate) (ABTS). The peak of ABTS oxidation was obtained approximately 1 day after the peak of MnP activity. Conclusions: ABTS oxidation was not caused by manganese peroxidase (MnP) nor by laccase but was the consequence of the action of Mn(III) which was stabilised in the growth medium. Decomposition of the complex took place after the biomass was removed from the growth medium and especially after the aeration of the culture was interrupted. Signi®cance and Impact of the Study: Mn(III) seems to be the cause of false positive laccase reactions. More reliable data on MnP activity can be obtained if the complex is decomposed by the fungus before MnP activity is measured in the medium.

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 98%

Laccase production by Phanerochaete chrysosporium - an artefact caused by Mn(III)?

Podgornik¹,

Stegu²,

Zibert³

et al. 2001

Lett Appl Microbiol

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“…First, manganese is known to be required to induce MnP production in many white rot fungi, including Bjerkandera sp. strain BOS55 (34,40,43). Manganese was shown to regulate MnP synthesis at the transcriptional level in the well-studied white rot fungus P. chrysosporium (8).…”

Section: Discussionmentioning

confidence: 99%

Manganese Is Not Required for Biobleaching of Oxygen-Delignified Kraft Pulp by the White Rot Fungus Bjerkandera sp. Strain BOS55

Moreira

Sierra-Álvarez

Lema

et al. 1997

Appl Environ Microbiol

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The white rot fungus Bjerkandera sp. strain BOS55 extensively delignified and bleached oxygen-delignified eucalyptus kraft pulp handsheets. Biologically mediated brightness gains of up to 14 ISO (International Standards Organization units) were obtained, providing high final brightness values of up to 80% ISO. In nitrogen-limited cultures (2.2 mM N), manganese (Mn) greatly improved manganese-dependent peroxidase (MnP) production. However, the biobleaching was not affected by the Mn nutrient regimen, ranging from 1,000 M added Mn to below the detection limit of 0.26 M Mn in EDTA-extracted pulp medium. The lowest Mn concentration tested was at least several orders of magnitude lower than the K m known for MnP. Consequently, it was concluded that Mn is not required for biobleaching in Bjerkandera sp. strain BOS55. Nonetheless, fast protein liquid chromatography profiles indicated that MnP was the predominant oxidative enzyme produced even under culture conditions in the near absence of manganese. High nitrogen (22 mM N) and exogenous veratryl alcohol (2 mM) repressed biobleaching in Mn-deficient but not in Mn-sufficient culture medium. No correlation was observed between the titers of extracellular peroxidases and the biobleaching. However, the decolorization rate of the polyaromatic dye Poly R-478 was moderately correlated to the biobleaching under a wide range of Mn and N nutrient regimens.

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“…The presence of these isoforms was confirmed by PAGE. Multiple forms of MnP [13] and laccase [14] have also been reported in other ligninolytic fungi. Previously, only one MnP protein and one laccase protein were detected in crude culture supernatants of L. edodes grown in submerged culture with 2.6 mM‐N [9].…”

Section: Resultsmentioning

confidence: 99%

Effect of nutrient nitrogen and manganese on manganese peroxidase and laccase production by Pleurotus sajor-caju

Buswell

2006

FEMS Microbiology Letters

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Pleurotus sajor‐caju, strain Pl‐27, produces manganese‐dependent peroxidase (MnP) and laccase, but not lignin peroxidase, when grown on a defined medium with glucose as sole carbon source. MnP activity was detected in fungal cultures supplemented with both high (26 mM‐N) and low (2.6 mM‐N) nutrient nitrogen although higher specific activity values were recorded under the latter conditions. Conversely, laccase production was not influenced by nutrient nitrogen levels under the growth conditions adopted. Both the titre and time of appearence of MnP were also affected by the concentration of Mn in the culture medium with highest enzyme levels recorded in cultures supplemented with 15 ppm Mn. Two MnP and five laccase isoforms were identified by FPLC and gel electrophoresis.

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Manganese peroxidases of the white rot fungus Phanerochaete sordida

Cited by 100 publications

References 43 publications

Laccase production by Phanerochaete chrysosporium - an artefact caused by Mn(III)?

Laccase production by Phanerochaete chrysosporium - an artefact caused by Mn(III)?

Manganese Is Not Required for Biobleaching of Oxygen-Delignified Kraft Pulp by the White Rot Fungus Bjerkandera sp. Strain BOS55

Effect of nutrient nitrogen and manganese on manganese peroxidase and laccase production by Pleurotus sajor-caju

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