Effect of nutrient nitrogen and manganese on manganese peroxidase and laccase production by Pleurotus sajor-caju

Fu, Shi Yu; Yu, Hui-sheng; Buswell, John A.

doi:10.1111/j.1574-6968.1997.tb10232.x

Cited by 47 publications

(19 citation statements)

References 14 publications

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“…Amino acids or their ramifications are always used as N sources to enhance the laccase activity of white-rot fungi. L-Asparagine can be absorbed directly by organismal cells and participates in metabolic activity (Fu et al 1997;Arora et al 2001;Dhawan et al 2002). Results presented in this paper show that appropriate concentrations of additive N sources could enhance the laccase activity of P. simplicissimum in solid-state fermentation, though the enhancement was not the results of higher fungal biomass.…”

Section: Discussionmentioning

confidence: 73%

Laccase activities of a soil fungus Penicillium simplicissimum in relation to lignin degradation

Zeng

Huang

et al. 2006

World J Microbiol Biotechnol

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The laccase activities of Penicillium simplicissimum H5 during solid-state fermentation with rice straw were studied. Degradation of lignocellulose was also followed. Results showed that all supplemental carbon sources inhibited the laccase activity in different degrees, while suitable concentrations of supplemental nitrogen sources remarkably enhanced the laccase activity. The enhancement of activity by the ordinary laccase inducers 2, 2¢-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and xylidine was not observed in this study. Lignocellulose degradation was improved when laccase activity was relatively low, suggesting a polymerizing function of laccase in lignin degradation by P. simplicissimum.

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Section: Discussionmentioning

confidence: 73%

Laccase activities of a soil fungus Penicillium simplicissimum in relation to lignin degradation

Zeng

Huang

et al. 2006

World J Microbiol Biotechnol

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show abstract

“…For example, 2,6-dimethoxyphenol (SHUTTLEWORTH et al 1986), guaiacol (VARES et al 1995 and ABTS (FU et al 1997, MUÑOZ et al 1997 were all used to detect laccase activity respectively. It has been found in this study that o-tolidine is the best substrate to analyze laccase zymogram while compared with 1,4-diaminobenzene, guaiacol and ABTS.…”

Section: Discussionmentioning

confidence: 99%

Production of lignocellulolytic enzymes by Trametes gallica and detection of polysaccharide hydrolase and laccase activities in polyacrylamide gels

Sun

Zhang

2004

J. Basic Microbiol.

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White rot fungus Trametes gallica was studied for the production of lignocellulolytic enzymes: cellulase, xylanase, laccase, manganese-dependent peroxidase (MnP), and lignin peroxidase (LiP). The results demonstrated that low-nitrogen (2.2 mM N) and surface stationary cultivation favored production of extracellular MnP. MnP activity reached 118.1 UL(-1) while T. gallica was grown in a low-nitrogen culture containing phenylalanine. However, laccase levels observed in high-nitrogen (22 mM N) agitated cultures were much greater than those seen in low-nitrogen. The N source experiments seemed to reveal that NH4+ plays an important role in inducing MnP and laccase of the fungus. Results showed that T. gallica produces a series of the lignocellulolytic enzymes, and needs high N to produce all the enzymes during solid-state fermentation of wheat straw. This paper also presents a modified zymogram procedure to detect xylanase and laccase of T. gallica in polyacrylamide gel. Xylanase in crude enzyme of T. gallica was displayed by contacting protein gel strips with xylan substrate gels and by staining with iodine. By immersing the protein gel strips in o-tolidine solution, the blue-green zones representing laccase activity were visualized against a colorless background.

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“…Laccases are widely distributed among fungi, where they have been assigned roles in lignin degradation (Bourbonnais et al 1995;Eggert et al 1997), in rendering phenolic compounds less toxic via oxidative coupling and polymerisation (Bollag et al 1988; Moldes and Sanroman 2006) and in mushroom fruit-body morphogenesis (Zhao and Kwan 1999;Chen et al 2004). Laccases may be either constitutive or inducible, and many factors including culture parameters (Fu et al 1997), heavy metals (Baldrian and Gabriel 2002;Chen et al 2003) and aromatic compounds (Scheel et al 2000) are reported to influence enzyme production in different fungal species.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cu2+, Mn2+ and aromatic compounds on the production of laccase isoforms by Coprinus comatus

Lu¹,

Ding²

2010

Mycoscience

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Six different extracellular laccase isoforms were identified in submerged cultures of the commercially important edible mushroom, Coprinus comatus. Although laccase activity (*55 IU/L) was readily detectable in unsupplemented control cultures containing 1.6 lM Cu 2? after 22-day incubation, mean enzyme levels (*150-185 IU/L) were 2.7-3.4-fold higher in cultures supplemented with 0.5-3.0 mM Cu 2? . Laccase production was also stimulated by Mn supplementation over the range 0.05-0.8 mM Mn 2? , and the peak value of *225 IU/L recorded after 22 days in cultures containing 0.8 mM added Mn 2? was 4.5-fold higher compared with unsupplemented controls. Of 12 aromatic compounds tested for their effect on laccase isozyme production by C. comatus, highest laccase levels (*188 IU/L), equivalent to a 4.4-fold increase compared with unsupplemented controls (*43 IU/L), were recorded after 22 days in cultures supplemented with 3.0 mM caffeic acid. Other aromatic compounds tested all stimulated laccase production, with peak enzyme levels 1.3-3.3-fold higher compared with unsupplemented controls. Extracellular laccase levels in cultures supplemented with optimal concentrations of Mn 2? and caffeic acid together were 38% and 15% lower, respectively, compared with cultures containing the separate supplements. Lac1 was the most abundant laccase isoform produced under all the conditions tested, but marked differences were observed in the production patterns of Lac2-Lac6.

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Effect of nutrient nitrogen and manganese on manganese peroxidase and laccase production by Pleurotus sajor-caju

Cited by 47 publications

References 14 publications

Laccase activities of a soil fungus Penicillium simplicissimum in relation to lignin degradation

Laccase activities of a soil fungus Penicillium simplicissimum in relation to lignin degradation

Production of lignocellulolytic enzymes by Trametes gallica and detection of polysaccharide hydrolase and laccase activities in polyacrylamide gels

Effect of Cu2+, Mn2+ and aromatic compounds on the production of laccase isoforms by Coprinus comatus

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