Manganese‐enhanced MRI of mouse heart during changes in inotropy

Hu, Tom C.‐C.; Pautler, Robia G.; MacGowan, Guy A.; Koretsky, Alan P.

doi:10.1002/mrm.1273.abs

Cited by 48 publications

(106 citation statements)

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“…channels activity, using T1-imaging at baseline and after infusion of dobutamine. Signal enhancement relates then to calcium influx and inotropic response [8,23,68,168].…”

Section: Manganese-enhanced Mri (Memri)mentioning

confidence: 99%

High field magnetic resonance imaging of rodents in cardiovascular research

et al. 2016

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Transgenic and gene knockout rodent models are primordial to study pathophysiological processes in cardiovascular research. Over time, cardiac MRI has become a gold standard for in vivo evaluation of such models. Technical advances have led to the development of magnets with increasingly high field strength, allowing specific investigation of cardiac anatomy, global and regional function, viability, perfusion or vascular parameters. The aim of this report is to provide a review of the various sequences and techniques available to image mice on 7-11.7 T magnets and relevant to the clinical setting in humans. Specific technical aspects due to the rise of the magnetic field are also discussed.

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“…channels activity, using T1-imaging at baseline and after infusion of dobutamine. Signal enhancement relates then to calcium influx and inotropic response [8,23,68,168].…”

Section: Manganese-enhanced Mri (Memri)mentioning

confidence: 99%

High field magnetic resonance imaging of rodents in cardiovascular research

et al. 2016

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“…However, several studies indicate that Mn 2+ uptake is related to the specific function of CaSR in a given tissue [25][26][27][28][29][30][31][32][33][34]. This implies that manganese-enhanced magnetic resonance imaging (MEMRI) of human breast cancer, could take advantage from a careful, tissue-specific timing of MRI acquisition and from improved postprocessing of images.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, Mn 2+ can enter cells via the same transport systems in the same way as Ca 2+ and can bind to a number of intracellular structures because of its high affinity for Ca 2+ and Mg 2+ binding sites on proteins and nucleic acids [23,24]. Mn 2+ ion is increasingly used for MRI in animals and it has been found to be a good contrast medium for MRI of the brain [25,26] and the heart [27,28]. Finally, the olfactory [29], visual [30,31], and somatosensory pathways of rat and mouse brain have been visualised in detail after direct injection of MnCl 2 into a specific brain region [32][33][34].…”

Section: Introductionmentioning

confidence: 99%

In vivo imaging of human breast cancer mouse model with high level expression of calcium sensing receptor at 3T

Baio¹,

Fabbi²,

Emionite³

et al. 2011

Eur Radiol

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“…To date its exact mechanism of action is still not completely comprehended, but it is suggested to act as a Ca 2+ analogue. 14,26,56 Its effects on mouse cardiac function have not been addressed extensively, with its molecular mechanisms on intracellular targets being largely unknown and unproved. Based on experimental results from a series of invasive catheterizations, this study examines the temporal stability of hemodynamic indices of systolic and diastolic function in C57BL/6 mice under isoflurane (ISO) anesthesia conditions (employing the same protocol widely used in MRI/microCT studies), and the effects of Mn 2+ on the mouse hemodynamic response as a potential modulator of myocardial force generation.…”

Section: Abbreviationsmentioning

confidence: 99%

“…Its importance, secondary to its potential role as an inotropic agent, lies in its ability to function as a T 1 contrast agent in magnetic resonance imaging (MRI), 13,26 as a viability, 51 or as a neuronal marker. 41,47 It is known as an intracellular agent, rapidly up-taken (within minutes 8,29,54 ) by L-type Ca 2+ channels, 26 and released extremely slowly [within time intervals ‡5-10 min] (with reported excretion half-times of a fast and a slow component of 4 and 39 days, respectively 29 ) from the intracellular space via the Na + -Ca 2+ exchanger.…”

Section: Abbreviationsmentioning

confidence: 99%

Murine Cardiac Hemodynamics Following Manganese Administration Under Isoflurane Anesthesia

2011

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This study examines (a) the temporal stability of hemodynamic indices of systolic and diastolic function in C57BL/6 mice under 1.5% isoflurane (ISO) (v/v) anesthesia conditions in 50:50 O(2)/N(2)O (v/v) within 90 min post-induction, and (b) the effects of Mn(2+) on the mouse hemodynamic response in male C57BL/6 mice (n = 16). Left ventricular catheterizations allowed estimation of the hemodynamic indices. Hypertonic saline infusion (10%) allowed absolute volume quantification in conjunction with a separate series of aortic flow experiments (n = 3). In a separate cohort of mice (n = 6), MnCl(2) (190 nmoles/g/bw) was infused via the left jugular for 29-39 min, following 11 min of baseline recording, to assess temporal responses. Stable temporal hemodynamic responses were achieved in control mice under ISO anesthesia. Hemodynamic indices during control, time-matched-control, baseline-Mn, and Mn-infused periods, were within normal expected ranges. No chronotropic changes were observed. Significant differences in systolic and diastolic cardiac indices of function (HR, EF, ESP, dP/dt (max), dP/dt (min), PAMP, τ(glantz), and τ(weiss)) resulted between baseline-Mn and Mn-infused time periods in Mn-treated mice at the 1% significance (p < 0.001). Transient positive, or negative, or positive followed by negative evoked pressure-volume loop shifts were observed (exemplified through changes in the end-systolic pressure-volume relationship and dP/dt (max)) in Mn-infusion studies. It is concluded that Mn(2+) can be used safely for prolonged mouse imaging studies, however, the significant variations elicited in cardiovascular hemodynamics post-manganese infusion, necessitate further investigations for its suitability and appropriateness for quantification of global cardiac function in image-based phenotyping.

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Manganese‐enhanced MRI of mouse heart during changes in inotropy

Cited by 48 publications

References 0 publications

High field magnetic resonance imaging of rodents in cardiovascular research

High field magnetic resonance imaging of rodents in cardiovascular research

In vivo imaging of human breast cancer mouse model with high level expression of calcium sensing receptor at 3T

Murine Cardiac Hemodynamics Following Manganese Administration Under Isoflurane Anesthesia

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