High field magnetic resonance imaging of rodents in cardiovascular research

Vanhoutte, Laetitia; Gerber, Bernhard; Gallez, Bernard; Po, Chrystelle; Magat, Julie; Balligand, Jean‐Luc; Feron, Olivier; Moniotte, Stéphane

doi:10.1007/s00395-016-0565-2

Cited by 13 publications

(11 citation statements)

References 179 publications

(184 reference statements)

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“…For this reason, gradient echocardiography-based imaging techniques are used ( 97 ). MRI systems for mice have small bore magnets imaging at very high field strengths (>7 T) ( 188 ). Increasing field strength for MRI increases the signal-to-noise ratio, resulting in higher resolution.…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

“…Cardiac MRI benefits from unrestricted spatial access to the myocardium, allowing long-axis imaging of two, three, or four chambers and continuous stacks of serial short-axis images for reproducible measurements of ventricular volumes and masses ( 9 , 167 , 184 ). Current instruments allow for a temporal resolution of 10–20 phases/heart beat (i.e., 5–10 ms) and a spatial resolution of 0.1–0.2 mm ( 188 ). A stack of six to eight serial, 1-mm-thick, short-axis slices is recommended to cover the entire LV and RV ( 184 , 197 ).…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

“…This is achieved by late Gd-enhancement imaging after injection of a contrast agent ( 92 ). The technique is based on the principle that Gd chelates have an extravascular distribution volume ( 188 ). Late Gd enhancement images are acquired at least 10 min after an intravenous or intraperitoneal injection of the contrast agent.…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

See 2 more Smart Citations

Guidelines for measuring cardiac physiology in mice

Lindsey

Kassiri

Virag

et al. 2018

American Journal of Physiology-Heart and Circulatory Physiology

251

194

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Cardiovascular disease is a leading cause of death, and translational research is needed to understand better mechanisms whereby the left ventricle responds to injury. Mouse models of heart disease have provided valuable insights into mechanisms that occur during cardiac aging and in response to a variety of pathologies. The assessment of cardiovascular physiological responses to injury or insult is an important and necessary component of this research. With increasing consideration for rigor and reproducibility, the goal of this guidelines review is to provide best-practice information regarding how to measure accurately cardiac physiology in animal models. In this article, we define guidelines for the measurement of cardiac physiology in mice, as the most commonly used animal model in cardiovascular research.Listen to this article’s corresponding podcast at http://ajpheart.podbean.com/e/guidelines-for-measuring-cardiac-physiology-in-mice/.

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Section: Magnetic Resonance Imagingmentioning

confidence: 99%

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

See 1 more Smart Citation

Guidelines for measuring cardiac physiology in mice

Lindsey

Kassiri

Virag

et al. 2018

American Journal of Physiology-Heart and Circulatory Physiology

251

194

View full text Add to dashboard Cite

show abstract

“…In the majority of cases in animal research, this is routinely performed by echocardiography [64]. Other methods that are frequently used to assess cardiac function are magnetic resonance imaging (MRI) [65] and measurement of LV pressure changes, by using a micromanometer [66]. Cardiac function was assessed in most of the studies included in this systematic review, as discussed below.…”

Section: Effects Of Interventions In Wnt Signaling On Cardiac Functionmentioning

confidence: 99%

Effect of Interventions in WNT Signaling on Healing of Cardiac Injury: A Systematic Review

Daskalopoulos

Blankesteijn

2021

Cells

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The wound healing that follows myocardial infarction is a complex process involving multiple mechanisms, such as inflammation, angiogenesis and fibrosis. In the last two decades, the involvement of WNT signaling has been extensively studied and effects on virtually all aspects of this wound healing have been reported. However, as often is the case in a newly emerging field, inconsistent and sometimes even contradictory findings have been reported. The aim of this systematic review is to provide a comprehensive overview of studies in which the effect of interventions in WNT signaling were investigated in in vivo models of cardiac injury. To this end, we used different search engines to perform a systematic search of the literature using the key words “WNT and myocardial and infarction”. We categorized the interventions according to their place in the WNT signaling pathway (ligand, receptor, destruction complex or nuclear level). The most consistent improvements of the wound healing response were observed in studies in which the acylation of WNT proteins was inhibited by administering porcupine inhibitors, by inhibiting of the downstream glycogen synthase kinase-3β (GSK3β) and by intervening in the β-catenin-mediated gene transcription. Interestingly, in several of these studies, evidence was presented for activation of cardiomyocyte proliferation around the infarct area. These findings indicate that inhibition of WNT signaling can play a valuable role in the repair of cardiac injury, thereby improving cardiac function and preventing the development of heart failure.

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“…The field of cardiovascular MR (CMR), in both research and clinical arenas, has evolved rapidly over the past decade. CMR applications include assessment of cardiac anatomy, regional wall motion, myocardial perfusion, myocardial viability, cardiac function assessment, assessment of myocardial infarction, and myocardial injury (Vanhoutte et al, ). CMR is increasingly used to characterize genetic and pharmacological mouse models (Sosnovik et al, ; Ebrahimi et al, ; Gotschy et al, ).…”

Section: Cardiac Mrimentioning

confidence: 99%

Diverse application of MRI for mouse phenotyping

2017

Birth Defects Research

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Small animal models, particularly mouse models, of human diseases are becoming an indispensable tool for biomedical research. Studies in animal models have provided important insights into the etiology of diseases and accelerated the development of therapeutic strategies. Detailed phenotypic characterization is essential, both for the development of such animal models and mechanistic studies into disease pathogenesis and testing the efficacy of experimental therapeutics. Magnetic Resonance Imaging (MRI) is a versatile and non-invasive imaging modality with excellent penetration depth, tissue coverage, and soft tissue contrast. MRI, being a multi-modal imaging modality, together with proven imaging protocols and availability of good contrast agents, is ideally suited for phenotyping mutant mouse models. Here we describe the applications of MRI for phenotyping structural birth defects involving the brain, heart, and kidney in mice. The versatility of MRI and its ease of use are well suited to meet the rapidly increasing demands for mouse phenotyping in the coming age of functional genomics.

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High field magnetic resonance imaging of rodents in cardiovascular research

Cited by 13 publications

References 179 publications

Guidelines for measuring cardiac physiology in mice

Guidelines for measuring cardiac physiology in mice

Effect of Interventions in WNT Signaling on Healing of Cardiac Injury: A Systematic Review

Diverse application of MRI for mouse phenotyping

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