Mancozeb fungicide-induced genotoxic effects, metabolic alterations, and histological changes in the colon and liver of Sprague Dawley rats

Abstract

The present study was designed to evaluate genotoxic markers of mancozeb exposure and withdrawal in colon and liver tissues together with histological changes in the gastrointestinal tract of Sprague Dawley rats. Thirty rats were divided into three equal groups; group I: treatment, 250 mg/kg mancozeb dissolved in corn oil administered twice weekly for 7 weeks; group II: withdrawal, the same treatment as group I after which animals were untreated for 5 weeks; group III: control, administered corn oil on the same schedule as group I for 7 weeks. All administrations were by oral gavage. Serum samples were analyzed for biochemical parameters. The comet assay and histopathological examinations were done on liver and colon specimens. The results demonstrated that mancozeb exposure caused significant increases in triglycerides and total cholesterol accompanied by decreases in glucose levels, with extensive DNA damage in liver and colon together with pathological changes in stomach, colon, and liver. Mancozeb withdrawal for 5 weeks improved the lipid and glucose profiles and decreased the degree of DNA damage and changes in the architecture of the stomach, colon, and liver. We concluded that discontinuing exposure to mancozeb fungicide for 5 weeks could ameliorate the adverse effects induced by 7 weeks of exposure to mancozeb. A longer withdrawal time may further reduce the observed genotoxicity.

Mancozeb impairs mitochondrial and bioenergetic activity in Drosophila melanogaster

“…MZ exposure for 15 days increased triglycerides and reduced glucose, glycogen, and trehalose. These results corroborate with Yahia et al (Yahia et al, 2014(Yahia et al, , 2019, who found that MZ increased triglycerides and decreased serum glucose in rats exposed to this fungicide. In the present study, MZ induced weight loss in flies.…”

“…Studies carried out with rodents demonstrated that exposure to MZ could alter triglycerides and glucose levels in the serum (Yahia et al, 2019). This fact could lead to metabolic disturbances.…”

Histopathological, immunohistochemical and biochemical alterations in liver tissue after fungicide-mancozeb exposures in Wistar albino rats

“…Yahia et al 37 used 250-mg/kg mancozeb dissolved in corn oil administered twice weekly for seven weeks and indicated that mancozeb exposure caused an increment in triglycerides, and total cholesterol also decreased glucose levels, resulting in the damage of DNA in the liver and colon with pathological changes in stomach, colon, and liver. In the light of this study, we used a single dosage of 500-mg/kg mancozeb dissolved in corn oil.…”

Cytotoxicity of mancozeb on Sertoli–germ cell co-culture system: Role of MAPK signaling pathway

“…Many previous studies have also reported adverse health effects of MZB in experimental mammals such as hepatotoxicity (Saber et al, 2019;Yahia et al, 2019), carcinogenic potential (Belpoggi et al, 2002), neurotoxicity and Parkinson-like symptoms (Domico et al, 2007), dysfunction of thyroid glands (Axelstad et al, 2011), nephrotoxicity, endocrine system disruption in females, and defects in fetal development (Bianchi et al, 2020;Girish and Reddy, 2017;Liu et al, 2017). Male reproductive system disorders such as infertility and spermatogenesis insufficiency has been reported previously.…”