Mancozeb impairs mitochondrial and bioenergetic activity in Drosophila melanogaster

Abstract

Mancozeb (MZ) is a broad-spectrum fungicide used worldwide in several crops. Neurological disorders in humans and animals have been associated with exposure to this compound by mechanisms still not fully understood. Drosophila melanogaster represents a reliable model in toxicological studies, presenting genetic and biochemical similarities with mammals. In this study, D. melanogaster flies were exposed for 15 days to MZ through the food (5 and 10 mg/mL). After that period, the efficiency of mitochondrial respiration complexes and metabolic markers were analyzed and evaluated. Flies presented weight loss, lower glucose, trehalose, and glycogen levels, and augmented levels of triglycerides concerning control (non-treated group). Acetyl-CoA Synthetase (ACeCS-1) and Acyl-Coenzyme Synthetase (ACSL1) contents were unchanged by MZ treatment. Mitochondrial respiration of flies was targeted by MZ treatment, evidenced by a decrease in oxygen consumption and bioenergetics rate and inhibition in mitochondrial complexes I/II. These results suppose that an impairment in mitochondrial respiration jointly with reduced levels of energetic substrates might be a mechanism involved in MZ deleterious effects, possibly by the limitation of ATP's availability, necessary for essential cellular processes.

Drosophila melanogaster as a Biotechnological Tool to Investigate the Close Connection Between Fatty Diseases and Pesticides

“…Vol.67: e24230091, 2024 www.scielo.br/babt Cont. Table 2 2021 Genetic basis of susceptibility to low-dose paraquat and variation between the sexes in Drosophila melanogaster [106] 2021 Oxidative stress and decreased dopamine levels induced by imidacloprid exposure cause behavioral changes in a neurodevelopmental disorder model in Drosophila melanogaster [107] 2021 Mancozeb impairs mitochondrial and bioenergetic activity in Drosophila melanogaster [108] 2020…”

Removal of ethylene bisdithiocarbamate fungicides in wastewater and agricultural runoff by zinc oxide nanoparticles before analysis by HPLC and UV-Vis spectroscopy

“…Dithiocarbamate pes ticides like mancozeb (MCZ) are regarded as significant fungicides with applications worldwide by farmers from various countries [8]. Despite the wide application and acceptance of (MCZ) fungicides, it was found to be lethal and detrimental to off-target essential organismandhumanbeings.Mancozebwasfound to impart neurotoxicity, damaging mitochondria, andmyofibrilsplitting [9], thyroid disruption [10], changes in glutathione and alterations in essential metal homeos tasis [11], adverse effects on s teroid production [12], ovarian injury and apoptosis [13], induce alteration in the development of hypothalamic sys tems that are essential for maturation of neuroendocrine sys tem [14], disrupt mitochondria and bioenergetic activities in the body [15], affect male fertility [16] and induce behavioural deficit [17].Although(MCZ)iswidelyacceptedandused by farmers in many countries, these and many other reports have proven that the uncontrolled and excessiveapplicationof(MCZ)fungicideisacase for alarm. Therefore, there is an urgent and crucial need to devise a means through which the residues ofthesetoxicpes ticidescanbereducedordegraded in the environment.…”

Assessment of Mancozeb Exposure, Absorbed Dose, and Oxidative Damage in Greenhouse Farmers

“…Additionally, ETU is employed as an accelerator in rubber vulcanization [ 13 ]. As a mechanism of action, ETU disrupts protein synthesis and metabolism by inactivating amino acidic sulfhydryl groups in the protein structure of fungal pathogens [ 14 ]. ETU is produced as a contaminant or as degradation product when fungicides are stored in the presence of moisture and oxygen.…”