Managing meiotic recombination in plant breeding

Wijnker, Erik; Jong, Hans de

doi:10.1016/j.tplants.2008.09.004

Cited by 109 publications

(101 citation statements)

References 54 publications

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“…Because of its relevance, regardless of the method, the recombination index has been widely discussed and estimated elsewhere (Forni-Martins 1996, BaptistaGiacomelli et al 2000. Recombination is a crucial event in genetic improvement for allowing the creation of new allele combinations in the chromosomes of genotypes to be improved (Martinez-Perez andMoore 2008, Wijnker andJong 2008).…”

Section: Resultsmentioning

confidence: 99%

Meiotic behavior of wild Caricaceae species potentially suitable for papaya improvement

Silva

Neto

Pereira

et al. 2012

Crop Breed. Appl. Biotechnol.

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Section: Resultsmentioning

confidence: 99%

Meiotic behavior of wild Caricaceae species potentially suitable for papaya improvement

Silva

Neto

Pereira

et al. 2012

Crop Breed. Appl. Biotechnol.

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“…Identifying factors influencing meiotic CO frequency are undoubtedly important for refining breeding strategies because hyperrecombinant lines could be selected to facilitate breeding programs (44). Increased MRRs in euchromatic chromosome arms could contribute to accelerated selection for novel haplotypes without the need for very large populations.…”

Section: Discussionmentioning

confidence: 99%

Loss of DNA methylation affects the recombination landscape in Arabidopsis

Mirouze

Lieberman‐Lazarovich²,

Aversano³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

174

137

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During sexual reproduction, one-half of the genetic material is deposited in gametes, and a complete set of chromosomes is restored upon fertilization. Reduction of the genetic information before gametogenesis occurs in meiosis, when cross-overs (COs) between homologous chromosomes secure an exchange of their genetic information. COs are not evenly distributed along chromosomes and are suppressed in chromosomal regions encompassing compact, hypermethylated centromeric and pericentromeric DNA. Therefore, it was postulated that DNA hypermethylation is inhibitory to COs. Here, when analyzing meiotic recombination in mutant plants with hypomethylated DNA, we observed unexpected and counterintuitive effects of DNA methylation losses on CO distribution. Recombination was further promoted in the hypomethylated chromosome arms while it was inhibited in heterochromatic regions encompassing pericentromeric DNA. Importantly, the total number of COs was not affected, implying that loss of DNA methylation led to a global redistribution of COs along chromosomes. To determine by which mechanisms altered levels of DNA methylation influence recombination-whether directly in cis or indirectly in trans by changing expression of genes encoding recombination components-we analyzed CO distribution in wild-type lines with randomly scattered and wellmapped hypomethylated chromosomal segments. The results of these experiments, supported by expression profiling data, suggest that DNA methylation affects meiotic recombination in cis. Because DNA methylation exhibits significant variation even within a single species, our results imply that it may influence the evolution of plant genomes through the control of meiotic recombination.epigenetic | chromatin | epigenetic recombinant inbred lines | met1-3 R egulation of meiotic recombination, as with other essential chromosomal activities like transcription and replication, depends on both DNA sequence and chromatin properties (1, 2). Although regulatory aspects of meiotic recombination have been studied in great detail, it is still not well understood how chromatin structure influences the frequency and distribution of recombination events, as reflected by the final number and distribution of cross-overs (COs) along chromosomes. Biased chromosomal positioning of COs has been recognized for many years; indeed, COs are most likely to occur in euchromatic chromosomal arms, distal to the recombinationally suppressed pericentromeric heterochromatin. These two chromatin compartments are characterized by differences in the abundance of genes and transposable elements (TEs). TEs accumulate in pericentromeric regions, whereas genes are enriched in distal euchromatin. Because suppressive epigenetic marks are primarily directed at silencing TEs, these two chromatin types also differ in their epigenetic signatures. Pericentromeric chromatin is enriched in the methylation of histone H3 at lysine 9 (H3K9me) and encompasses hypermethylated DNA. In contrast, distal chromatin exhibits active marks such as acet...

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“…Boosting crossovers would effectively speed up the combining of valuable traits from different parents in new elite varieties (Wijnker and de Jong, 2008), accelerate the removal of linkage drag during the introgression of valuable genes from exotic germplasms (Able and Langridge, 2006;Martinez-Perez and Moore, 2008), and facilitate the construction of highly recombinogenic lines and thus help improve genetic maps and positional cloning techniques. Recently, Carlton et al (2006) showed that the presence of a single pair of univalents during meiosis of Caenorhabditis elegans induces a compensatory increase in crossovers on the recombining chromosomes involved in bivalents of the same nuclei.…”

Section: Introductionmentioning

confidence: 99%

Crossovers Get a Boost inBrassicaAllotriploid and Allotetraploid Hybrids

et al. 2010

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Meiotic crossovers are necessary to generate balanced gametes and to increase genetic diversity. Even if crossover number is usually constrained, recent results suggest that manipulating karyotype composition could be a new way to increase crossover frequency in plants. In this study, we explored this hypothesis by analyzing the extent of crossover variation in a set of related diploid AA, allotriploid AAC, and allotetraploid AACC Brassica hybrids. We first used cytogenetic methods to describe the meiotic behavior of the different hybrids. We then combined a cytogenetic estimation of class I crossovers in the entire genome by immunolocalization of a key protein, MutL Homolog1, which forms distinct foci on meiotic chromosomes, with genetic analyses to specifically compare crossover rates between one pair of chromosomes in the different hybrids. Our results showed that the number of crossovers in the allotriploid AAC hybrid was higher than in the diploid AA hybrid. Accordingly, the allotetraploid AACC hybrid showed an intermediate behavior. We demonstrated that this increase was related to hybrid karyotype composition (diploid versus allotriploid versus allotetraploid) and that interference was maintained in the AAC hybrids. These results could provide another efficient way to manipulate recombination in traditional breeding and genetic studies.

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Managing meiotic recombination in plant breeding

Cited by 109 publications

References 54 publications

Meiotic behavior of wild Caricaceae species potentially suitable for papaya improvement

Meiotic behavior of wild Caricaceae species potentially suitable for papaya improvement

Loss of DNA methylation affects the recombination landscape in Arabidopsis

Crossovers Get a Boost inBrassicaAllotriploid and Allotetraploid Hybrids

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