Meiotic behavior of wild Caricaceae species potentially suitable for papaya improvement

Silva, Emanuelli Narducci da; Neto, Monique Freitas; Pereira, Telma Nair Santana; Pereira, Messias Gonzaga

doi:10.1590/s1984-70332012000100007

Cited by 9 publications

(7 citation statements)

References 29 publications

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“…Heilborn (1921) reported 2n = 18 for Carica papaya, Vasconcellea pubescens, and the hybrid Vasconcelllea × heilbornii. The same number was reported for Jacaratia spinosa (Kumar and Srinivasan 1944;Silva et al 2012), Vasconcellea goudotiana, V. microcarpa, V. monoica (de Zerpa, 1959, and V. quercifolia (Bernardello et al 1990). More recent studies confirmed 2n = 18 for these species (Costa et al 2008;Damasceno et al 2009;Silva et al 2012) and reported the same number for four additional species of Vasconcellea (V. cauliflora, V. longiflora, V. palandensis, V. sphaerocarpa;Caetano et al 2008;Costa et al 2008;Damasceno et al 2009;Silva et al 2012).…”

Section: Introductionsupporting

confidence: 66%

V. Chromosome Counts for the Caricaceae Reveal Unexpected Dysploidy

Carvalho

Rockinger

Sousa

et al. 2015

Molecular Phylogeny, Biogeography and an E-Monograph of the Papaya Family (Caricaceae) as an Example of Taxonomy in the Electro

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Section: Introductionsupporting

confidence: 66%

V. Chromosome Counts for the Caricaceae Reveal Unexpected Dysploidy

Carvalho

Rockinger

Sousa

et al. 2015

Molecular Phylogeny, Biogeography and an E-Monograph of the Papaya Family (Caricaceae) as an Example of Taxonomy in the Electro

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“…Bajpai and Singh (2006), working on the meiosis of ten papaya cultivars, observed that meiosis was very disturbed and many meiotic irregularities were recorded, with chromosome stickiness being the most common (44%), followed by chromatin bridges (21.1%), laggard chromosomes (4.8%), chromosome diminution/degenera- tion (5.2%), and micronucleus (5.9%). Silva et al (2012). also working with Caricaceae wild species (V. goudotiana, V. quercifolia, and J. spinosa), observed lagging chromosomes, sticky chromosomes, precocious segregation, and fiber disturbance.…”

Section: Discussionmentioning

confidence: 96%

“…On the other hand, the male genotype had an MI equal to 76.9%, which is a low value; the genotype can therefore be considered meiotically unstable (Love 1951). Meiotic index below 90% has been reported for Caricaceae as V. monoica (Damasceno Jr. et al 2010) and V. goudotiana (Silva et al 2012).…”

Section: Discussionmentioning

confidence: 98%

Meiotic Behavior of Male and Hermaphrodite Genotypes of Papaya (<i>Carica papaya</i> L.)

et al. 2013

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SummaryThe aim of this study was to analyze the meiotic behavior and to estimate the meiotic index and the pollen grain viability of three papaya genotypes, male (Cariflora) and hermaphrodite (Golden and UENF/CALIMAN01). In considering of the fact that the genotypes have different flower sizes, initially, meiotic stages were defined in relation to bud size, and, after that, meiosis was observed in the three genotypes. To observe meiosis, flower buds of different sizes were collected and the length of each bud was measured using graph paper. Once the measurements had been taken, two slides were prepared for each flower bud by squashing the anthers in 1% acetic carmine solution. Slides were observed under an optical microscope and for each bud, size was determined as indicating one of three stages, depending on meiotic phase: pre-meiotic, meiotic stage, or post-meiotic stage. In this way, meiosis was observed for the three genotypes, with particular attention given to possible irregularities in cell division in relation to prophase I. It was observed that there is a relation between flower bud size and meiosis. In general, meiosis was normal, but all genotypes showed some meiotic irregularity, with laggard chromosome being the most common. The Cariflora (male) genotype had a higher frequency of irregularities compared to the hermaphrodite genotypes. The meiotic indices estimated for the Golden, UENF/CALIMAN01, and Cariflora genotypes were 92.6%, 91% and 76.9%, respectively; however, the pollen grain viability, on average, was higher than 90%. In conclusion, the meiotic behavior of male and hermaphrodite genotypes was similar, and all three genotypes had some degree of meiotic abnormality. Papaya (Carica papaya L.) is a diploid species with nine pairs of chromosomes (Damasceno Jr. et al. 2010). It is trioecious with female, male, and hermaphrodite plants, and is considered to be sexually polymorphic. The cultivars are ginoandromonoicas, consisting of female and hermaphrodite plants. Key wordsThe most accepted theory for sex determination in papaya plants is that the sex of plants is determined by a gene with three allelic forms (M 1 , M 2 , m); so, the genotype of the female plants is mm, that of the male is M 1 m and that of the hermaphrodite is M 2 m (Hofmeyr 1938, Storey 1938. However, Liu et al. (2004) formulated another theory that the sex of the plants is due to the presence of an incipient sex chromosome pair differentiated by a small male-specific region of the Y chromosome (MSY). According to Ming et al. (2007) there are two Y chromosomes in papaya, a controlling male designated as Y and the other, controlling the sex hermaphrodite, designated as Y h . The female plant is homogametic with XX chromosome constitution, the male is XY, and the hermaphrodite is XY h .

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“…In three wild species of Caricaceae family, Vasconcellea goudotiana, Vasconcellea quercifolia, and Jacaratia spinosa, which are very promising for papaya improvement programs due to the resistance to disease and cold, meiosis was partially typical, presenting pollen viability with satisfactory values ranging from 68% in V. goudotiana to 96% in J. spinosa (Silva et al, 2012). This study suggests that in breeding programs involving hybridization, the wild species V. goudotiana can be used as a maternal parent, as this species had the lowest MI and lower pollen viability, compared to the other studied species (Silva et al, 2012).…”

Section: Meiosis Study Associated With Plant Breedingmentioning

confidence: 99%

Research Article Contributions of classical and molecular cytogenetic in meiotic analysis and pollen viability for plant breeding.

et al. 2017

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ABSTRACT. The analysis of meiotic behavior has been widely used in the study of plants as they provide relevant information about the viability of a species. Meiosis boasts a host of highly conserved events and changes in genes that control these events will give rise to irregularities that can alter the normal course of meiosis and may lead to complete sterility of the plant. The recombination of genes that occur in meiosis is an important event to generate variability and has been important in studies for genetic improvement and to create viable hybrids. The use of fluorescence in situ hybridization and genomic in situ hybridization (GISH) in meiosis allows the localization of specific regions, enables to differentiate genomes in a hybrid, permits to observe the pairing of homoeologous chromosomes, and if there was a recombination between the genomes of progenitor species. Furthermore, the GISH allows us to observe the close relationship between the species involved. This article aims to report over meiosis studies on plants and hybrids, the use and importance of molecular cytogenetic in meiotic analysis and contributions of meiotic analysis in breeding programs.

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Meiotic behavior of wild Caricaceae species potentially suitable for papaya improvement

Abstract: -The purpose of this study was to evaluate the meiotic behavior and determine the meiotic index and

Cited by 9 publications

References 29 publications

V. Chromosome Counts for the Caricaceae Reveal Unexpected Dysploidy

V. Chromosome Counts for the Caricaceae Reveal Unexpected Dysploidy

Meiotic Behavior of Male and Hermaphrodite Genotypes of Papaya (<i>Carica papaya</i> L.)

Research Article Contributions of classical and molecular cytogenetic in meiotic analysis and pollen viability for plant breeding.

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