MAMP-triggered plant immunity mediated by the LysM-receptor kinase CERK1

Desaki, Yoshitake; Kohari, Masaki; Shibuya, Naoto; Kaku, Hanae

doi:10.1007/s10327-018-0828-x

Cited by 16 publications

(9 citation statements)

References 93 publications

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“…SFH3 encodes a lipid transfer protein (LTP) to which several roles in plant immunity have been assigned, e.g. early recognition of pathogen attacks [45, 117].…”

Section: Resultsmentioning

confidence: 99%

Weighted gene co-expression network analysis unveils gene networks associated with the Fusarium head blight resistance in tetraploid wheat

et al. 2019

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BackgroundFusarium head blight (FHB) resistance in the durum wheat breeding gene pool is rarely reported. Triticum turgidum ssp. carthlicum line Blackbird is a tetraploid relative of durum wheat that offers partial FHB resistance. Resistance QTL were identified for the durum wheat cv. Strongfield × Blackbird population on chromosomes 1A, 2A, 2B, 3A, 6A, 6B and 7B in a previous study. The objective of this study was to identify the defense mechanisms underlying the resistance of Blackbird and report candidate regulator defense genes and single nucleotide polymorphism (SNP) markers within these genes for high-resolution mapping of resistance QTL reported for the durum wheat cv. Strongfield/Blackbird population.ResultsGene network analysis identified five networks significantly (P < 0.05) associated with the resistance to FHB spread (Type II FHB resistance) one of which showed significant correlation with both plant height and relative maturity traits. Two gene networks showed subtle differences between Fusarium graminearum-inoculated and mock-inoculated plants, supporting their involvement in constitutive defense. The candidate regulator genes have been implicated in various layers of plant defense including pathogen recognition (mainly Nucleotide-binding Leucine-rich Repeat proteins), signaling pathways including the abscisic acid and mitogen activated protein (MAP) kinase, and downstream defense genes activation including transcription factors (mostly with dual roles in defense and development), and cell death regulator and cell wall reinforcement genes. The expression of five candidate genes measured by quantitative real-time PCR was correlated with that of RNA-seq, corroborating the technical and analytical accuracy of RNA-sequencing.ConclusionsGene network analysis allowed identification of candidate regulator genes and genes associated with constitutive resistance, those that will not be detected using traditional differential expression analysis. This study also shed light on the association of developmental traits with FHB resistance and partially explained the co-localization of FHB resistance with plant height and maturity QTL reported in several previous studies. It also allowed the identification of candidate hub genes within the interval of three previously reported FHB resistance QTL for the Strongfield/Blackbird population and associated SNPs for future high resolution mapping studies.

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“…SFH3 encodes a lipid transfer protein (LTP) to which several roles in plant immunity have been assigned, e.g. early recognition of pathogen attacks [45, 117].…”

Section: Resultsmentioning

confidence: 99%

Weighted gene co-expression network analysis unveils gene networks associated with the Fusarium head blight resistance in tetraploid wheat

et al. 2019

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“…Although they share common structural characteristics, these receptors are distinct in terms of recognized expression patterns and epitopes [ 23 , 25 , 52 , 62 ]. This shows that the receptors roles appear to have evolved independently in different groups of plants [ 25 , 71 ]. Therefore, considering that all candidate receptors in coffee, described in this study, increased their expression from 6 hpi in all evaluated cultivars, each one may have possible roles in the basal immunity of C .…”

Section: Discussionmentioning

confidence: 99%

“…Nonetheless, Bti9 is a membrane receptor with extracellular LysM motifs and high homology to At-CERK1. Furthermore, the At/Os-CERK1, besides playing a role as a receptor for fungal PAMPs, also participates as a co-receptor for PRRs in bacterial recognition [52,71], which demonstrates the multiple functions of this receptor and turns it into a possible target of bacterial and fungal effectors that suppress PTI. The Ca1 and 2 LYK 4 and 5, clades II and III, were grouped to grape receptors Vv-LYK4-1/ 2 and Vv-LYK5-1 (Fig 4).…”

Section: Joint Phylogenetic Analysismentioning

confidence: 99%

LysM receptors in Coffea arabica: Identification, characterization, and gene expression in response to Hemileia vastatrix

et al. 2022

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Pathogen‐associated molecular patterns (PAMPs) are recognized by pattern recognition receptors (PRRs) localized on the host plasma membrane. These receptors activate a broad-spectrum and durable defense, which are desired characteristics for disease resistance in plant breeding programs. In this study, candidate sequences for PRRs with lysin motifs (LysM) were investigated in the Coffea arabica genome. For this, approaches based on the principle of sequence similarity, conservation of motifs and domains, phylogenetic analysis, and modulation of gene expression in response to Hemileia vastatrix were used. The candidate sequences for PRRs in C. arabica (Ca1-LYP, Ca2-LYP, Ca1-CERK1, Ca2-CERK1, Ca-LYK4, Ca1-LYK5 and Ca2-LYK5) showed high similarity with the reference PRRs used: Os-CEBiP, At-CERK1, At-LYK4 and At-LYK5. Moreover, the ectodomains of these sequences showed high identity or similarity with the reference sequences, indicating structural and functional conservation. The studied sequences are also phylogenetically related to the reference PRRs described in Arabidopsis, rice, and other plant species. All candidates for receptors had their expression induced after the inoculation with H. vastatrix, since the first time of sampling at 6 hours post‐inoculation (hpi). At 24 hpi, there was a significant increase in expression, for most of the receptors evaluated, and at 48 hpi, a suppression. The results showed that the candidate sequences for PRRs in the C. arabica genome display high homology with fungal PRRs already described in the literature. Besides, they respond to pathogen inoculation and seem to be involved in the perception or signaling of fungal chitin, acting as receptors or co-receptors of this molecule. These findings represent an advance in the understanding of the basal immunity of this species.

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“…Nonetheless, Bti9 is a membrane receptor with extracellular LysM motifs and high homology to At-CERK1 . Furthermore, the At/Os-CERK1 , besides playing a role as a receptor for fungal PAMPs, also participates as a co-receptor for PRRs in bacterial recognition [53,72], which demonstrates the multiple functions of this receptor and turns it into a possible target of bacterial and fungal effectors that suppress PTI.…”

Section: Discussionmentioning

confidence: 99%

“…Although they share common structural characteristics, these receptors are distinct in terms of recognized expression patterns and epitopes [24,26,53,63]. This shows that the receptors roles appear to have evolved independently in different groups of plants [26,72]. Therefore, considering that all candidate receptors in coffee, described in this study, increased their expression from 6 hpi in all evaluated cultivars, each one may have possible roles in the basal immunity of C. arabica .…”

Section: Discussionmentioning

confidence: 99%

LysM receptors in Coffea arabica: identification, characterization, and gene expression in response to Hemileia vastatrix

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et al. 2021

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Pathogen‐associated molecular patterns (PAMPs) are recognized by pattern recognition receptors (PRRs) localized on the host plant cell wall. These receptors activate a broad-spectrum and durable defense, which are desired characteristics for disease resistance in plant breeding programs. In this study, candidate sequences for PRRs with lysin motifs (LysM) were investigated in the Coffea arabica genome. For this, approaches based on the principle of sequence similarity, conservation of motifs and domains, phylogenetic analysis, and modulation of gene expression in response to Hemileia vastatrix were used. The candidate sequences for PRRs in C. arabica ( Ca1-LYP , Ca2-LYP , Ca1-CERK1 , Ca2-CERK1 , Ca-LYK4 , Ca1-LYK5 and Ca2-LYK5 ) showed high similarity with the reference PRRs used: Os-CEBiP , At-CERK1 , At-LYK4 and At-LYK5 . Moreover, the ectodomains of these sequences showed high identity or similarity with the reference sequences, indicating structural and functional conservation. The studied sequences are also phylogenetically related to the reference PRRs described in Arabidopsis, rice, and other plant species. All candidates for receptors had their expression induced after the inoculation with H. vastatrix , since the first time of sampling at 6 hours post‐inoculation (hpi). At 24 hpi, there was a significant increase in expression, for most of the receptors evaluated, and at 48 hpi, a suppression. The results showed that the candidate sequences for PRRs in the C. arabica genome display high homology with fungal PRRs already described in the literature. Besides, they respond to pathogen inoculation and seem to be involved in the perception or signaling of fungal chitin, acting as receptors or coreceptors of this molecule. These findings represent an advance in the understanding of the basal immunity of this species.

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MAMP-triggered plant immunity mediated by the LysM-receptor kinase CERK1

Cited by 16 publications

References 93 publications

Weighted gene co-expression network analysis unveils gene networks associated with the Fusarium head blight resistance in tetraploid wheat

Weighted gene co-expression network analysis unveils gene networks associated with the Fusarium head blight resistance in tetraploid wheat

LysM receptors in Coffea arabica: Identification, characterization, and gene expression in response to Hemileia vastatrix

LysM receptors in Coffea arabica: identification, characterization, and gene expression in response to Hemileia vastatrix

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