Mammary gland cell’s tight junction permeability from dairy cows producing stable or unstable milk in the ethanol test

Stumpf, Marcelo Tempel; Fischer, Vívian; Daltro, D. S.; Alfonzo, Evelyn Priscila München; Kolling, G. J.; Silva, Marcos Vinícius Gualberto Barbosa da; Pereira, L. G. R.; McManus, Concepta

doi:10.1007/s00484-020-01967-0

Cited by 3 publications

(3 citation statements)

References 9 publications

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“…In the transition goats, the circulating electrolytes such as Na, Cl, Mg, and K were assumed to change following the different distribution of fluids in late gestation, birthing, and early lactation [ 5 ], while the loss of the TJ integrity in the goat mammary gland during lactation was closely related to milk reduction and the disturbance of the epithelia barrier [ 6 , 7 , 8 ]. Cows producing unstable milk presented elevated tight junction permeability [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Tight Junctions by Sex Hormones in Goat Mammary Epithelial Cells

Zhu

Jia

Zhang

et al. 2022

Animals

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The sex hormones of estrogen and progesterone (P4) play a vital role in mammary gland development and milk lactation in ruminants. The tight junction (TJ) between adjacent secretory epithelial cells is instrumental in establishing the mammary blood–milk barrier. However, whether estrogen and P4 exert their effect on mammary function via regulating TJ remain unclear. Here, to clarify the role of 17-β estradiol (E2) and P4 in the regulation of TJ in goat mammary gland, we first explored the relationships between the concentrations of E2, P4, and the protein expression of claudin-1, claudin-3, occludin, and ZO-1 during the mammary gland development in goat. Then, we further explored the mRNA and protein expression of claudin-1, claudin-3, occludin, and ZO-1 in the goat mammary epithelial cells (GMECs) in vitro under different concentrations of E2 and P4. The results demonstrated that the protein expression of claudin-1 decreased, but occludin and ZO-1 increased with the decline in E2 and P4 during the transition from pregnancy to lactation. In the in vitro studies, E2 exerted a positive effect on the mRNA expression of claudin-1, and accelerated the proteins’ expression of claudin-1 and ZO-1 in GMECs; P4 upregulated the mRNA expression of claudin-1, claudin-3, occludin, and ZO-1, and also improved the protein expression of claudin-1, claudin-3, and ZO-1 in the GMECs. The results demonstrated that E2 and P4 play an important role in regulating the expression of the mammary TJ components, which may ultimately affect the mammary gland development and milk lactation.

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Section: Introductionmentioning

confidence: 99%

Regulation of Tight Junctions by Sex Hormones in Goat Mammary Epithelial Cells

Zhu

Jia

Zhang

et al. 2022

Animals

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“…Several lines of evidence indicate that various membrane proteins in BMECs, including immune sensors (Toll-like receptors, TLR), nutrient transporters (glucose transporter and aquaporin), and tight junction proteins (claudin and occludin), are involved in the onset of mastitis or milk production. 39 , 40 …”

Section: Resultsmentioning

confidence: 99%

“…Using the STRING database, we eliminated all background proteins in all pathways regulated by ubiquitination to more clearly visualize the PPIs (protein-protein interactions) between the significantly altered proteins (Figure 7A). 39,40 Previous studies applying omics technologies to dairy cows have largely omitted posttranslational regulation. 41,42 The present extensive proteomic investigation of the ubiquitinome in BMECs infected with S. agalactiae provides insights on the regulatory roles and mechanisms of protein ubiquitination in the mammary gland in dairy cows and a reference for the development of novel biomarkers for mastitis therapy.…”

Section: Functional Enrichment Analysis Of the Identified Ubiquitinat...mentioning

confidence: 99%

The Analysis of the Ubiquitylomic Responses to Streptococcus agalactiae Infection in Bovine Mammary Gland Epithelial Cells

Tong¹,

Ji²,

Zhang³

et al. 2022

JIR

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Streptococcus agalactiae is one of the primary pathogens responsible for subclinical mastitis, a significant economic burden for dairy farms. An essential component of the immune response to infection is ubiquitination, which plays important roles in the complex interactions between the pathogen and host. Materials and Methods:In the present study, quantitative ubiquitylomics was performed to profile changes in the global ubiquitinome of bovine mammary gland epithelial cells (BMECs) infected with S. agalactiae. Results: The most notable changes in the BMEC ubiquitinome were related to the adherens junction, ribosome, and tight junction pathways. Ubiquitination of CTNNB1, EGFR, ITGB1, CTNNA1, CTNNA2, CDH1, YES1, and SLC9A3R1 appears to be fundamental for regulating multiple cellular processes in BMECs in response to S. agalactiae infection. In addition, broad ubiquitination of various effectors and outer membrane proteins was observed. Ubiquitinated proteins in S. agalactiae-infected BMECs were associated with regulating cell junctions in the host, with potential implications for susceptibility to infection. Conclusion:The preliminary findings suggest that extensive ubiquitination of CTNNB1, CDH1 and SLC9A3R1 and proteins closely related to cell junctions might play an important role in mastitis progression in dairy cows. The results provide evidence that ubiquitin modification of certain proteins in S. agalactiae-infected BMECs could be a promising therapeutic strategy for reducing mammary gland injury and mastitis.

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