Mammalian glucose permease GLUT1 facilitates transport of arsenic trioxide and methylarsonous acid

Liu, Zijuan; Sánchez, Marco A.; Jiang, Xuan; Boles, Eckhard; Landfear, Scott M.; Rosen, Barry P.

doi:10.1016/j.bbrc.2006.10.054

Cited by 121 publications

(80 citation statements)

References 41 publications

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“…[17] Should MA V be taken up by plants in a similar way the lower As concentration in root after exposure to MA V would be understandable, since then MA V would first need to be reduced to MA III at the root/soil interface. Differences in redox potential between As V and MA V could then, among other things, explain the differences in uptake efficiency for these two As species.…”

Section: Total Arsenic Concentration In Root and Shootmentioning

confidence: 99%

Uptake and translocation of inorganic and methylated arsenic species by plants

et al. 2007

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Environmental context. The molecular occurrence of arsenic in soils can vary as a result of soil conditions and/or application of arsenic-containing herbicides or fertiliser. Although large amounts of As-containing herbicides are used for different crops, there is still a lack of understanding as to how the molecular form of As determines the uptake of arsenic into plants and, in particular, the translocation into shoot and grain.Abstract. The uptake and translocation into shoots of arsenate, methylarsonate (MA), and dimethylarsinate (DMA) by 46 different plant species were studied. The plants (n = 3 per As species) were exposed for 24 h to 1 mg of As per litre under identical conditions. Total arsenic was measured in the roots and the shoots by acid digestion and inductively coupled plasma mass spectrometry from which, besides total As values, root absorption factors and shoot-to-root transfer factors were calculated. As uptake into the root for the different plant species ranged from 1.2 to 95 (µg of As per g of dry weight) for As V , from 0.9 to 44 for MA V and from 0.8 to 13 for DMA V , whereas in shoots the As concentration ranged from 0.10 to 17 for As V , 0.1 to 13 for MA V , and 0.2 to 17 for DMA V . The mean root absorption factor for As V (1.2 to 95%) was five times higher than for DMA V (0.8 to 13%) and 2.5 times higher than for MA V (0.9 to 44%). Although the uptake of arsenic in the form of As V was significantly higher than that of MA V and DMA V , the translocation of the methylated species was more efficient in most plant species studied. Thus, an exposure of plants to DMA V or MA V can result in higher arsenic concentrations in the shoots than when exposed to As V . Shoot-to-root transfer factors (TFs) for all plants varied with plant and arsenic species. While As V had a median TF of 0.09, the TF of DMA V was nearly a factor of 10 higher (0.81). The median TF for MA V was in between (0.30). Although the TF for MA V correlates well with the TF for DMA V , the plants can be separated into two groups according to their TF of DMA V in relation to their TF of As V . One group can immobilise DMA V in the roots, while the other group translocates DMA V very efficiently into the shoot. The reason for this is as yet unknown.

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Section: Total Arsenic Concentration In Root and Shootmentioning

confidence: 99%

Uptake and translocation of inorganic and methylated arsenic species by plants

et al. 2007

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“…The budding yeast Saccharomyces cerevisiae has proved to be an excellent eukaryotic model to study the mechanisms of arsenic tolerance and also a tool to identify and characterize mammalian tolerance factors (12,13,19,20,24). Although detoxification mechanisms are well studied, little is known about how yeast detects the presence of arsenic-induced stress signals, how this information is transduced, and what the downstream targets of such responses are.…”

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confidence: 99%

Mitogen-Activated Protein Kinase Hog1 Mediates Adaptation to G₁Checkpoint Arrest during Arsenite and Hyperosmotic Stress

et al. 2008

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Cells slow down cell cycle progression in order to adapt to unfavorable stress conditions. Yeast (Saccharomyces cerevisiae) responds to osmotic stress by triggering G 1 and G 2 checkpoint delays that are dependent on the mitogen-activated protein kinase (MAPK) Hog1. The high-osmolarity glycerol (HOG) pathway is also activated by arsenite, and the hog1⌬ mutant is highly sensitive to arsenite, partly due to increased arsenite influx into hog1⌬ cells. Yeast cell cycle regulation in response to arsenite and the role of Hog1 in this process have not yet been analyzed. Here, we found that long-term exposure to arsenite led to transient G 1 and G 2 delays in wild-type cells, whereas cells that lack the HOG1 gene or are defective in Hog1 kinase activity displayed persistent G 1 cell cycle arrest. Elevated levels of intracellular arsenite and "cross talk" between the HOG and pheromone response pathways, observed in arsenite-treated hog1⌬ cells, prolonged the G 1 delay but did not cause a persistent G 1 arrest. In contrast, deletion of the SIC1 gene encoding a cyclin-dependent kinase inhibitor fully suppressed the observed block of G 1 exit in hog1⌬ cells. Moreover, the Sic1 protein was stabilized in arsenite-treated hog1⌬ cells. Interestingly, Sic1-dependent persistent G 1 arrest was also observed in hog1⌬ cells during hyperosmotic stress. Taken together, our data point to an important role of the Hog1 kinase in adaptation to stress-induced G 1 cell cycle arrest.

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“…Thus, the pathogenesis of hyperglycemia and occurrence of renal disease is slightly less. although previous studies indicated that GLUT1 transports arsenic as well as glucose in erythrocytes, in blood-brain barrier and in epithelial cells (12,18), the association between this GLUT1 and the concentration or transport of arsenic in cells has not been studied yet.…”

Section: Discussionmentioning

confidence: 98%

“…ıncrease of blood glucose level causes hyperglycemia, which induces some changes in kidney such as increase of glomerular mesangial synthesis, renal hypertrophy, glomerulosclerosis and accumulation of extracellular matrix proteins. Furthermore, GLUT1 transports arsenic compounds right along with glucose in erythrocytes, blood-brain barrier, epithelial cells, tubular and glomerular part of the kidney, and, thus, it may be an important protein in the risk of diabetes in case of chronic arsenic exposure (11,12).…”

Section: Introductionmentioning

confidence: 99%

Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 gene in Turkish Population

et al. 2016

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Glucose transporter 1 (Glut1) is located in basolateral side of cells and mediates the unidirectional transport of glucose as non-insulin dependent glucose transporter protein. ıt is responsible for transportation of glucose especially to cancer cells and also transports arsenic compounds that is related with diabetes mellitus. The present study aimed to detect the allele and genotype frequencies of Glut1 -2841a>t single nucleotide polymorphism (SNP) in turkish population. Blood samples were collected from unrelated 100 male and 110 female. Polymerase Chain reaction (PCr)-restriction Fragment length Polymorphism (rFlP) technique was used to analyze the blood samples. The distribution of -2841a>t SNP was 55.2%, 38.1% and 6.7% for aa, at and tt genotype, respectively. The frequency of the a allele was found as 74.3% and those of the t allele as 25.7%. The statistical evaluation represented that the allele frequencies was accordant with Hardy-Weinberg equilibrium (χ2 =0.002, p=0.97). This was the first study examining the frequencies of Glut1 -2841a>t polymorphism in turkish population. ın our opinion, these results can be used in epidemiological studies both in turkey and other countries.

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Mammalian glucose permease GLUT1 facilitates transport of arsenic trioxide and methylarsonous acid

Cited by 121 publications

References 41 publications

Uptake and translocation of inorganic and methylated arsenic species by plants

Uptake and translocation of inorganic and methylated arsenic species by plants

Mitogen-Activated Protein Kinase Hog1 Mediates Adaptation to G₁Checkpoint Arrest during Arsenite and Hyperosmotic Stress

Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 gene in Turkish Population

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Mammalian glucose permease GLUT1 facilitates transport of arsenic trioxide and methylarsonous acid

Cited by 121 publications

References 41 publications

Uptake and translocation of inorganic and methylated arsenic species by plants

Uptake and translocation of inorganic and methylated arsenic species by plants

Mitogen-Activated Protein Kinase Hog1 Mediates Adaptation to G1Checkpoint Arrest during Arsenite and Hyperosmotic Stress

Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 gene in Turkish Population

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Mitogen-Activated Protein Kinase Hog1 Mediates Adaptation to G₁Checkpoint Arrest during Arsenite and Hyperosmotic Stress