Mammalian Food Safety Risk Assessment of Transgenic Cotton Containing Cry1Ac Gene Conducted Independently in Pakistan

Rahman, Mehboob-ur; Zaman, Muhammad Arif Uz; Zafar, Yusuf; Hayder, Zulfiqar; Jamil, Amer; Kikuchi, Akira; Watanabe, Kazuo

doi:10.4172/2167-0331.1000122

Cited by 3 publications

(1 citation statement)

References 35 publications

(53 reference statements)

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“…Our biosafety study on wister rats, which involved using 40% GM cottonseed as a diet in (G3) group and 30% Non-GM cottonseed as diet in (G2) group, showed no comparable change of harmful effect on LFT, RFT and serum electrolyte when compared to the standard diet control group (G1). These results are consistent with the previous studies 20 , 36 .…”

Section: Discussionsupporting

confidence: 94%

Engineered resistance and risk assessment associated with insecticidal and weeds resistant transgenic cotton using wister rat model

Iqbal

Ali

Ahmed

et al. 2022

Sci Rep

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Stacking multiple genes into cotton crop to cop up multiple biotic stresses such as insects and weeds is a promising tool to save crop from losses. Transgenic cotton variety, VH-289, with double Bt and cp4EPSPS genes under the control of 35S promoter was used for the expression analyses and biosafety studies. The transgenic cotton plants were screened through PCR amplification of fragments, 1.7 kb for Cry1Ac, 582 bp for Cry2A and 250 bp for cp4EPSPS; which confirmed the presence of all genes transformed in transgenic cotton. The Cry1Ac + Cry2A and cp4EPSPS proteins were quantified through ELISA in transgenic cotton plants. The Glyphosate assay performed by spraying 1900 mL per acre of glyphosate Roundup further confirmed complete survival of transgenic cotton plants as compared to the non-transgenic cotton plants and all weeds. Similarly, insect infestation data determined that almost 99% insect mortality was observed in controlled field grown transgenic cotton plants as compared to the non-transgenic control plants. Evaluation of effect of temperature and soil nutrients availability on transgene expression in cotton plants was done at two different cotton growing regions, Multan and Lahore, Pakistan and results suggested that despite of higher temperature in Multan field, an increased level of Cry and cp4EPSPS proteins was recorded due to higher soil organic matter availability compared to Lahore field. Before commercialization of any transgenic variety its biosafety study is mandatory so, a 90 days biosafety study of the transgenic cotton plants with 40% transgenic cottonseeds in standard diet showed no harmful effect on wister rat model when studied for liver function, renal function and serum electrolyte.

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Section: Discussionsupporting

confidence: 94%

Engineered resistance and risk assessment associated with insecticidal and weeds resistant transgenic cotton using wister rat model

Iqbal

Ali

Ahmed

et al. 2022

Sci Rep

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A 90−day subchronic toxicity study of transgenic cotton expressing Cry1Ac, Cry2A and CP4−EPSPS proteins in Sprague−Dawley rats

Ali

Salisu

Shahid

et al. 2020

Food and Chemical Toxicology

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Biosafety and toxicity assessment of transgenic cotton-harboring insecticide and herbicide tolerant genes on albino mice

Bhutta,

Awais,

Raouf

et al. 2024

Toxicology Research

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Introduction Genetic engineering has revolutionized agriculture by transforming biotic and abiotic stress-resistance genes in plants. The biosafety of GM crops is a major concern for consumers and regulatory authorities. Methodology A 14-week biosafety and toxicity analysis of transgenic cotton, containing 5 transgenes ((Cry1Ac, Cry2A, CP4 EPSPS, VIP3Aa, and ASAL)), was conducted on albino mice. Thirty mice were divided into three groups (Conventional, Non-transgenic, without Bt, and transgenic, containing targeted crop) according to the feed given, with 10 mice in each group, with 5 male and 5 female mice in each group. Results During the study, no biologically significant changes were observed in the non-transgenic and transgenic groups compared to the control group in any of the study’s parameters i.e. increase in weight of mice, physiological, pathological, and molecular analysis, irrespective of the gender of the mice. However, a statistically significant change was observed in the hematological parameters of the male mice, while no such change was observed in the female study group mice. The expression analysis, however, of the TNF gene increases many folds in the transgenic group as compared to the non-transgenic and conventional groups. Conclusion Overall, no physiological, pathological, or molecular toxicity was observed in the mice fed with transgenic feed. Therefore, it can be speculated that the targeted transgenic crop is biologically safe. However, more study is required to confirm the biosafety of the product on the animal by expression profiling.

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Mammalian Food Safety Risk Assessment of Transgenic Cotton Containing Cry1Ac Gene Conducted Independently in Pakistan

Cited by 3 publications

References 35 publications

Engineered resistance and risk assessment associated with insecticidal and weeds resistant transgenic cotton using wister rat model

Engineered resistance and risk assessment associated with insecticidal and weeds resistant transgenic cotton using wister rat model

A 90−day subchronic toxicity study of transgenic cotton expressing Cry1Ac, Cry2A and CP4−EPSPS proteins in Sprague−Dawley rats

Biosafety and toxicity assessment of transgenic cotton-harboring insecticide and herbicide tolerant genes on albino mice

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