Mammalian cells exposed to ionizing radiation: Structural and biochemical aspects

Sabanero, Myrna; Azorín-Vega, Juan Carlos; Flores-Villavicencio, Lérida Liss; Domínguez, José Pedro Castruita; Hernández, Miguel Ángel Vallejo; Barbosa‐Sabanero, Gloria; Córdova–Fraga, Teodoro; Sosa-Aquino, Modesto Antonio

doi:10.1016/j.apradiso.2015.11.064

Cited by 6 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, ankyrin-1 (ANK1) alters the structure of the actin cytoskeleton and sustains limited cell migration during DNA damage 43 . Similar alterations in the arrangement of actin microfilaments and detachments from junctions were seen in HeLa cells exposed to IR and to ROS 44 . Such actin filaments rearrangements might be induced by RhoA, as rapid formation of actin filaments accompanied by redistribution of VE-cadherin adherent junctions in microvascular endothelial cells after radiation was mediated by RhoA/ROCK 12 .…”

Section: Discussionsupporting

confidence: 62%

Increased elasticity of melanoma cells after low-LET proton beam due to actin cytoskeleton rearrangements

Jasińska-Konior

Wiecheć

Sarna

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Cellular response to non-lethal radiation stress include perturbations in DNA repair, angiogenesis, migration, and adhesion, among others. Low-LET proton beam radiation has been shown to induce somewhat different biological response than photon radiation. For example, we have shown that non-lethal doses of proton beam radiation inhibited migration of cells and that this effect persisted long-term. Here, we have examined cellular elasticity and actin cytoskeleton organization in BLM cutaneous melanoma and Mel270 uveal melanoma cells. Proton beam radiation increased cellular elasticity to a greater extent than X-rays and both types of radiation induced changes in actin cytoskeleton organization. Vimentin level increased in BLM cells after both types of radiation. Our data show that cell elasticity increased substantially after low-LET proton beam and persisted long after radiation. This may have significant consequences for the migratory properties of melanoma cells, as well as for the cell susceptibility to therapy.

show abstract

Section: Discussionsupporting

confidence: 62%

Increased elasticity of melanoma cells after low-LET proton beam due to actin cytoskeleton rearrangements

Jasińska-Konior

Wiecheć

Sarna

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…After irradiation, micronucleus formation56 and cell lysis58 were observed. Additionally, cancer cells were reported to exhibit functional deficiency and reduced proliferation potential in response to γ‐radiation 59, 60…”

Section: Resultsmentioning

confidence: 99%

Radiation‐Sensitive Dendrimer‐Based Drug Delivery System

Chou

Yuh

et al. 2017

Advanced Science

View full text Add to dashboard Cite

Combination of chemotherapy and radiotherapy is used to enhance local drug delivery while reducing off‐target tissue effects. Anticancer drug doxorubicin (DOX) is loaded into l‐cysteine modified G4.5 dendrimer (GC/DOX) and released at different pH values in the presence and absence of γ‐radiation. Presence of γ‐radiation significantly improves DOX release from the GC/DOX under acidic pH conditions, suggesting that GC dendrimer is a radiation‐sensitive drug delivery system. GC/DOX is further evaluated by determining cytotoxicity in uterine cervical carcinoma HeLa cells. GC/DOX shows high affinity for cancer cells and effective drug release following an external stimulus (radiation exposure), whereas an in vivo zebrafish study confirms that l‐cysteine acts as a radiosensitizer. GC/DOX treatment combined with radiotherapy synergistically and successfully inhibits cancer cell growth.

show abstract

“…These results are entirely consistent with our initial assumption suggesting the ionizing effect on decreasing of the cellular stiffness. In fact, it can be concluded that X-rays have the potential for impact on the structure of macromolecules and cellular structural proteins due to ionizing properties (Sabanero et al, 2016). Therefore, it can be concluded that this is the ionizing effect which has made a change in the structure of the proteins of cytoskeleton and reduced the cell stiffness.…”

Section: Discussionmentioning

confidence: 99%

Effects of X-ray on fibroblast mechanical properties

Heydarian¹,

Khorramymehr²,

Vasaghi‐Gharamaleki³

2019

Journal of Theoretical and Applied Mechanics

View full text Add to dashboard Cite

Concerning the widespread use of X-rays to detect various diseases, such as oral and dental ones, it is essential to study the effects of this radiation on living cells. From the past, genetic effects and cell death because of X-rays have been studied. In addition, the effect of this ionizing radiation on the mechanical properties of the cell and cytoskeleton has been studied, but different results have been obtained based on different models. In this study, post-culture gingival fibroblast cells were classified into two groups of control and radiation with Nano Magnetic Particles functionalized by folic acid. The cells of the radiation group were exposed to X-rays of 3 mGy•cm 2. The specimens were undergone static creep test by a magnetic tweezer. Spring and damper coefficients were obtained based on the viscoelastic solid modeling. The static and dynamic stiffness of the groups was also calculated. The maximum deformation was decreased after radiation from 0.049 ± 0.01 µm to 0.029 ± 0.01 µm and the static stiffness of the model was 1.6 times decreased. Also, the gel point frequency for the control group was 27 Hz and for the radiation group was 7.5 Hz. The results show that the static and dynamic stiffness of the cells decreases after radiation, and less deformation appears in the cells after irradiation. These changes can be due to the breakdown of membrane chemical bonds and activation of actin fibers after radiation.

show abstract

Mammalian cells exposed to ionizing radiation: Structural and biochemical aspects

Cited by 6 publications

References 8 publications

Increased elasticity of melanoma cells after low-LET proton beam due to actin cytoskeleton rearrangements

Increased elasticity of melanoma cells after low-LET proton beam due to actin cytoskeleton rearrangements

Radiation‐Sensitive Dendrimer‐Based Drug Delivery System

Effects of X-ray on fibroblast mechanical properties

Contact Info

Product

Resources

About