Mammalian cell models to advance our understanding of wound healing: a review

Vidmar, Jerneja; Chingwaru, Constance; Chingwaru, Walter

doi:10.1016/j.jss.2016.10.016

Cited by 21 publications

(18 citation statements)

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“…In response to damage, the tissue undergoes a complicated process of healing, which consists of 3 stages: inflammation, proliferation, and maturation [136]. To facilitate normal wound healing, the use of an appropriate wound therapy and wound care intervention (bandages) is necessary [138,139]. Wounds that exhibit impaired healing by failure to progress through the normal stages of healing enter a state of pathologic inflammation and are considered chronic in nature.…”

Section: Wound Healingmentioning

confidence: 99%

“…In vitro models are generally simple, rapid, involve minimal ethical considerations, and provide insight into the biochemical and physiological processes induced by the test compound [137,142]. There are several standardized protocols, including proliferation [143], scratch [144], cell invasion [144], cell viability, proteomic, and genomic assays [139]. On the other hand, in vivo models are used to determine the degree of re-epithelialization, collagenation, neovascularization, and tensile or breaking strength of wounds.…”

Section: Wound Healingmentioning

confidence: 99%

“…Monoculture models of the epithelium offer a useful platform to study the re-epithelialization process. The cultures consist of keratinocytes (preferably HaCaT cells, an immortalized human skin cell line [147]), primary keratinocytes, or epidermal stem cells [139,[148][149][150][151]. The cell line is grown in a monolayer in vitro and is subjected to a number of assays relevant to the wound healing process [139].…”

Section: In Vitro Wound Healing Modelsmentioning

confidence: 99%

“…The cultures consist of keratinocytes (preferably HaCaT cells, an immortalized human skin cell line [147]), primary keratinocytes, or epidermal stem cells [139,[148][149][150][151]. The cell line is grown in a monolayer in vitro and is subjected to a number of assays relevant to the wound healing process [139]. Many studies have used the presence of cytokines [138,[152][153][154][155], growth factors [148,[156][157][158][159][160], or the expression of several markers of the ECM [161,162] as standards for the measurement of the wound healing processes [139].…”

Section: In Vitro Wound Healing Modelsmentioning

confidence: 99%

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Current Methods for the Discovery of New Active Ingredients from Natural Products for Cosmeceutical Applications

Espinosa-Leal

García‐Lara

2019

Planta Med

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Cosmeceuticals are designed to serve a dual purpose: to provide desired esthetical effects and to treat dermatological conditions. Natural products derived from plants and marine organisms are a novel source of potential cosmeceutical active ingredients for incorporation into new formulations due to consumer demands. Contrary to common perceptions, most regulatory agencies do not view cosmeceuticals as being a separate category from cosmetics; thus, these products are not regulated accordingly, thereby forcing the consumer to rely on the self-regulatory policies of the cosmetics industry. Cosmeceuticals are advertised as having capabilities that include anti-aging, anti-acne, solar-protective, wound healing, and skin whitening. Such traits normally comprise several biological activities. In order to ensure the safety and efficacy of these products, active ingredients employed in the formulations must undergo a series of tests. In this review, in vitro (enzymatic and cellular) and in vivo tests employed to evaluate the potential of new cosmeceutical active ingredients are discussed, and new trends that are being explored by the cosmeceutical industry are described.

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Section: Wound Healingmentioning

confidence: 99%

Section: Wound Healingmentioning

confidence: 99%

Section: In Vitro Wound Healing Modelsmentioning

confidence: 99%

Section: In Vitro Wound Healing Modelsmentioning

confidence: 99%

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Current Methods for the Discovery of New Active Ingredients from Natural Products for Cosmeceutical Applications

Espinosa-Leal

García‐Lara

2019

Planta Med

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“…A decisive sub-phase of wound healing is the regeneration of the blood vessel system and, thus, the restoration of a nutritive perfusion, without which healing of the wound would not occur or would be dramatically delayed. Therefore, the blood vessel is also called "a highway facilitating wound healing" [1].…”

Section: Introductionmentioning

confidence: 99%

Panta Rhei: Neovascularization, Angiogenesis and Nutritive Perfusion in Wound Healing

et al. 2018

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Background: In response to tissue damage, angiogenesis is an extremely dynamic process that is finely regulated by signals from cells, the surrounding extracellular matrix (ECM), and derived mediators. As the only process, angiogenesis remains of decisive importance in the context of the entire wound healing process and is subject to constant change. The dissolution of the endothelial basement membrane, the migration of endothelial cells, and the development of new capillary vessels during wound healing depend not only on the cells and cytokines present, but also on the production and organization of ECM components in the immediate wound. Summary: Angiogenesis in wound healing can be divided into two main phases. During the pro-angiogenic phase at the beginning of wound healing, excessive neo-formation of blood vessels, some of which are poorly differentiated, occurs, which restore blood flow and thus nutritive perfusion as quickly as possible. This is followed by an anti-angiogenic phase in which the initially established vascular network undergoes a maturing process, which, however, is accompanied by a significant reduction in the number of vessels. Key Messages: Although many mechanisms and specific cell functions in wound healing have already been described, many underlying pathophysiological processes remain unknown. Because angiogenesis and its maturation is a very fast but also very long-lasting process, the understanding of the underlying mechanisms is of crucial importance. This article will give an overview of the current understanding and controversy in this sub-step of wound healing.

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