Mammaglobin as a diagnostic serum marker of complex canine mammary carcinomas

Pandey, Mamta; Kumar, B. V. Sunil; Verma, Ramneek

doi:10.1016/j.rvsc.2015.10.010

Cited by 8 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IgG purified from the hyperimmune sera was used as primary antibody for indirect ELISA. The optimum dilution of primary antibody was selected by chequerboard titration against fixed dilution/concentration of recombinant antigen (Pandey et al 2015).…”

Section: Indirect Elisamentioning

confidence: 99%

Circulating level of heat shock protein 27 is elevated in dogs with mammary tumors

et al. 2019

View full text Add to dashboard Cite

In the current study, we have synthesized canine recombinant Hsp27 in E. coli and raised hyperimmune serum against the protein in mice. Using the hyperimmune serum, an indirect ELISA was developed to estimate circulating levels of Hsp27 in dogs with various types of mammary neoplasia and to compare their levels with those of tumor-free apparently healthy dogs. The developed assay had a high specificity (83.3%) and sensitivity (96.6%) for a cut-off value of 0.45 with respect to histopathological examination in discriminating healthy dogs from those bearing mammary tumors. Serum Hsp27 levels were found to be significantly elevated in tumor subjects (20.33 ± 1.23 ng/ml) as compared to healthy controls (6.56 ± 0.51 ng/ml) and the increase in the Hsp27 levels was irrespective of mammary tumor histotypes. However, dogs with grade-II tumors had higher Hsp27 levels as compared to grade-I types. Therefore, Hsp27 can be exploited as one of the 'neoplastic signatures' of canine mammary tumors.

show abstract

Section: Indirect Elisamentioning

confidence: 99%

Circulating level of heat shock protein 27 is elevated in dogs with mammary tumors

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Specificity and sensitivity of mammaglobin to detect human breast cancer makes it a promising biomarker [ 31 ]. Till now, to our best knowledge there is only one report from our laboratory in which we had investigated the potential of mammaglobin-B protein as a serum biomarker of canine mammary carcinomas [ 14 ]. However no information is available on its mRNA expression levels in CMT.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Previously we have cloned and heterologously expressed canine mammaglobin-B gene in E. coli as 12 kDa recombinant fusion protein and raised hyperimmune sera against the expressed protein in rabbits [ 14 ]. IgG purified from the hyperimmune sera [ 14 ] was used as primary antibody for IHC. Total proteins were extracted from the mammary tumor tissues [ 24 ] and Western blot [ 25 ] was carried out using the purified IgG to assess specificity of the raised antibodies.…”

Section: Methodsmentioning

confidence: 99%

“…So far, the mammaglobin expression studies are well reported for human breast cancers but no such reports are documented for mammary tumors in canines. In our previous study we reported detection of this protein for the first time in the serum of canines with mammary carcinomas [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Over-expression of mammaglobin-B in canine mammary tumors

et al. 2018

Self Cite

View full text Add to dashboard Cite

BackgroundMammaglobin, a member of secretoglobin family has been recognized as a breast cancer associated protein. Though the exact function of the protein is not fully known, its expression has been reported to be upregulated in human breast cancer.We focused on studying the expression of mammaglobin-B gene and protein in canine mammary tumor (CMT) tissue. Expression of mammaglobin-B mRNA and protein were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC), respectively.ResultsHigh levels of mammaglobin-B mRNA expression (6.663 ± 0.841times) was observed in CMT as compared to age and breed matched healthy controls. Further, expression of mammaglobin-B protein was detected in paraffin-embedded mammary tumor tissues from the same subjects by IHC. Mammaglobin-B protein was overexpressed only in 6.67% of healthy mammary glands while, a high level of its expression was scored in 76.7% of the CMT subjects. Moreover, no significant differences in terms of IHC score and qRT-PCR score with respect to CMT histotypes or tumor grades were observed, indicating that mammaglobin-B over-expression occurred irrespective of CMT types or grades.ConclusionOverall, significantly increased expression of mammaglobin-B protein was found in CMTs with respect to healthy mammary glands, which positively correlates to its transcript. These findings suggest that overexpression of mammaglobin-B is associated with tumors of canine mammary glands.

show abstract