MAMA-PCR assay for the detection of point mutations associated with high-level erythromycin resistance in Campylobacter jejuni and Campylobacter coli strains

Alonso, Rodrigo; Mateo, Estíbaliz; Churruca, Estibaliz; Martínez, Irati; Girbau, Cecilia; Fernández‐Astorga, Aurora

doi:10.1016/j.mimet.2005.03.013

Cited by 58 publications

(49 citation statements)

References 7 publications

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“…Probes for the detection of macrolide resistance in H. pylori were used [36,37]. The theoretical applicability of these probes for Campylobacter was assessed by controlling previous publications [38,39]. FISH may also be useful for detecting macrolide resistance in other bacteria, e.g.…”

Section: Erythromycinmentioning

confidence: 99%

Influence of Selected Per Orally Administered ATB on Microflora of GIT in Experimental Animals

Maďar¹,

Tatiana²

2018

Antibiotic Use in Animals

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Composition of gastrointestinal (GIT) microbiota difers in individual parts of GIT. Only 40% of GIT bacteria are cultivable. Fluorescence-in-situ-hybridization (FISH) can detect non-cultivable bacteria. Perorally administered antibiotics (ATB) afect the composition of microbiota in GIT. The absorbed ATB, namely penicillins, tetracyclines, macrolides or luorochinolons, have diferent inluence in comparison with poorly absorbed oral ATB, such as aminoglycosides, aminocoumarines or polypeptides. This efect is due to retention of high concentration of non-absorbed ATB during passage through GIT and their longer inluence on bacteria living in diferent parts of GIT. Study methods were based on scientiic literature review from PubMed, Elsevier databases and Slovak scientiic publications. We searched for publications between years 1980 and 2016, with keywords: ATB, inluence, microbiota, FISH. The literature review focuses on peroral administration of ATB to humans and animals and its potential efect on composition of GIT microbiota. The relevant studies showed that per orally administered ATB produced many important changes in microbiota of GIT. FISH method was more frequently used for screening the normal composition of microbiota than for studying the efects of ATB although there were some studies dealing also with this issue.

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Section: Erythromycinmentioning

confidence: 99%

Influence of Selected Per Orally Administered ATB on Microflora of GIT in Experimental Animals

Maďar¹,

Tatiana²

2018

Antibiotic Use in Animals

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“…MAMA-PCR has been largely used for timely nucleotide substitution identification in diverse settings (3,11,15). The combination of MC analysis resolution and the simplicity of MAMA-PCR-based assays provides a powerful tool for the accurate identification of SNPs without the need for extra steps after DNA amplification (13).…”

Section: Vol 49 2011mentioning

confidence: 99%

Interleukin-28B Genotyping by Melt-Mismatch Amplification Mutation Assay PCR Analysis Using Single Nucleotide Polymorphisms rs12979860 and rs8099917, a Useful Tool for Prediction of Therapy Response in Hepatitis C Patients

et al. 2011

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Several studies have identified associations between single nucleotide polymorphisms (SNPs) occurring near the interleukin-28B (IL-28B) gene and response to antiviral treatment among hepatitis C virus (HCV) patients. Here, we describe a reliable melt-mismatch amplification mutation assay (melt-MAMA) PCR-based genotyping method for IL-28B which can be used in the management of HCV patients, helping to better define the course of therapy.Hepatitis C virus (HCV) is a positive-polarity, singlestranded RNA virus belonging to the genus Hepacivirus in the family Flaviviridae (12). Worldwide, an estimated of three million new infections occur annually and approximately 130 million people have been infected, the vast majority of infections becoming chronic infections (4). Moreover, a significant number of infected patients develop severe liver disease, including cirrhosis and hepatocellular carcinoma (7,9,17). Currently, the first line of HCV antiviral therapy is based on administration of pegylated alpha interferon (PEG-IFN-␣) and ribavirin (RBV). Unfortunately, this therapeutic strategy is effective only in around 50% of patients infected with HCV genotype 1,

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“…Macrolides inhibit protein synthesis in the bacterial cell by binding to the 50S subunit of the ribosome (5). In Campylobacter, high-level resistance to macrolides is conferred by mutations of the 23S strand of the rRNA gene at the binding site of the macrolide (1,6,23). Similar mutations are responsible for resistance in Helicobacter pylori (20).…”

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confidence: 99%

“…Similar mutations are responsible for resistance in Helicobacter pylori (20). While different mutations occur at comparable frequencies in Helicobacter, the change of A to G at position 2059 (Escherichia coli equivalent; corresponds to position 2075 of Campylobacter) dominates macrolide resistance in Campylobacter (1,4,5,20,23). Rarely, efflux pumps are responsible for a low level of resistance that is of limited clinical relevance at standard antibiotic doses (3,5,12).…”

mentioning

confidence: 99%

“…The theoretical applicability of these probes for Campylobacter was checked by consulting previous publications (1,4,5,6,7,12,17,23) and by using the publicly available ARB program (www.ARB-home.de). The wild-type probe (C wt 23S [CGG GGT CTT TCC GTC TT]) was labeled with 6-carboxyfluorescein (6-FAM) and used in combination with a Cy3-labeled resistance probe (C res 23S 2059AϾG [CGG GGT CTC TCC GTC TT]) at a formamide concentration of 40% in the hybridization buffer and corresponding salt concentrations in the wash buffer as previously described for H. pylori (Thermo Hybaid, Ulm, Germany) (20,22,23).…”

mentioning

confidence: 99%

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Detection of Resistance to Macrolides in Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization

Haas

Essig

Bartelt³

et al. 2008

J Clin Microbiol

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MAMA-PCR assay for the detection of point mutations associated with high-level erythromycin resistance in Campylobacter jejuni and Campylobacter coli strains

Cited by 58 publications

References 7 publications

Influence of Selected Per Orally Administered ATB on Microflora of GIT in Experimental Animals

Influence of Selected Per Orally Administered ATB on Microflora of GIT in Experimental Animals

Interleukin-28B Genotyping by Melt-Mismatch Amplification Mutation Assay PCR Analysis Using Single Nucleotide Polymorphisms rs12979860 and rs8099917, a Useful Tool for Prediction of Therapy Response in Hepatitis C Patients

Detection of Resistance to Macrolides in Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization

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