Malvidin Protects WI-38 Human Fibroblast Cells Against Stress-induced Premature Senescence

Seo, Hye Rin; Choi, Mi Jin; Choi, Ji Myung; Ko, Jong Cheol; Ko, Jee Yeon; Cho, Eun Ju

doi:10.15430/jcp.2016.21.1.32

Cited by 20 publications

(20 citation statements)

References 46 publications

(60 reference statements)

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“…This result was similar to the anthocyanins components that were reported in blueberries [69], red rice [70] and grapes [71]. Malvidin has been shown to protect against cardiovascular disease [72], and attenuate H 2 O 2 -induced oxidative stress [73] and inflammation [74], demonstrating that this strain has great potential for medical application. Except for malvidin, other content of anthocyanin components in the purple pigments were not exactly the same as that of anthocyanin components reported in above-mentioned plants.…”

Section: Discussionsupporting

confidence: 85%

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

Zhang

Zeng

et al. 2020

BMC Genomics

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Background: Anthocyanins are common substances with many agro-food industrial applications. However, anthocyanins are generally considered to be found only in natural plants. Our previous study isolated and purified the fungus Aspergillus sydowii H-1, which can produce purple pigments during fermentation. To understand the characteristics of this strain, a transcriptomic and metabolomic comparative analysis was performed with A. sydowii H-1 from the second and eighth days of fermentation, which confer different pigment production. Results: We found five anthocyanins with remarkably different production in A. sydowii H-1 on the eighth day of fermentation compared to the second day of fermentation. LC-MS/MS combined with other characteristics of anthocyanins suggested that the purple pigment contained anthocyanins. A total of 28 transcripts related to the anthocyanin biosynthesis pathway was identified in A. sydowii H-1, and almost all of the identified genes displayed high correlations with the metabolome. Among them, the chalcone synthase gene (CHS) and cinnamate-4hydroxylase gene (C4H) were only found using the de novo assembly method. Interestingly, the best hits of these two genes belonged to plant species. Finally, we also identified 530 lncRNAs in our datasets, and among them, three lncRNAs targeted the genes related to anthocyanin biosynthesis via cis-regulation, which provided clues for understanding the underlying mechanism of anthocyanin production in fungi. Conclusion: We first reported that anthocyanin can be produced in fungus, A. sydowii H-1. Totally, 31 candidate transcripts were identified involved in anthocyanin biosynthesis, in which CHS and C4H, known as the key genes in anthocyanin biosynthesis, were only found in strain H1, which indicated that these two genes may contribute to anthocyanins producing in H-1. This discovery expanded our knowledges of the biosynthesis of anthocyanins and provided a direction for the production of anthocyanin.

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Section: Discussionsupporting

confidence: 85%

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

Zhang

Zeng

et al. 2020

BMC Genomics

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“…The alkaloids fraction ( Table 3) consisted mainly of indicine-N-oxide (52.1%), ellipcine (8.1%), crinane-3α-ol (4.5%), augustamine (4.4%), 1β,2βepoxyambelline (3.8%), cinchonine (3.8%), 13α-hydrorhombifoline (3.5%), dihydro-oxo-demethoxyhaemanthamine (3.0%), oxoassoamine (3.0%), caffeine (2.3%), augustifoline (2.1%), 9-octadecinamide (2.0%), theobromine (2.0%), thalicarpin (1.7%), lupanine (1.4%), and crinamidine (1.3%). Malvidin possesses antioxidant, anti-inflammatory, cardioprotective, antihypertensive, and antitumor properties (Huang, Zhu, Li, Sui, & Min, 2016;Quintieri et al, 2013;Seo et al, 2016). β-Carotene is an antioxidant and antiosteoporotic agent (Rao & Rao, 2007), and a major precursor of vitamin A (Agea et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…β-Carotene is an antioxidant and antiosteoporotic agent (Rao & Rao, 2007), and a major precursor of vitamin A (Agea et al, 2014). Malvidin possesses antioxidant, anti-inflammatory, cardioprotective, antihypertensive, and antitumor properties (Huang, Zhu, Li, Sui, & Min, 2016;Quintieri et al, 2013;Seo et al, 2016). Therefore, a great number of potentially active molecules present in the leaves of Talinum triangulare, as well as the multifunctional properties these compounds, make Talinum triangulare a good source of healthpromoting substances.…”

Section: Resultsmentioning

confidence: 99%

Bioactive phytochemicals in an aqueous extract of the leaves of Talinum triangulare

Ikewuchi

Ifeanacho

2016

Food Science & Nutrition

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An aqueous leaf extract of Talinum triangulare was screened for the presence of bioactive molecules, using gas chromatography coupled with pulse and flame ionization detectors. It had high carotenoids; moderate benzoic acid derivatives, hydroxycinnamates and flavonoids; and low terpenes, alkaloids, phytosterols, allicins, glycosides, saponins, and lignans contents. Ten known carotenoids (mainly 50.42% carotene and 33.30% lycopene), nine benzoic acid derivatives (mainly 84.63% ferulic acid and 11.92% vanillic acid), and six hydroxycinnamates (55.44% p‐coumaric acid and 44.46% caffeic acid) were detected. Also detected were eight lignans (88.02% retusin) and thirty flavonoids (50.35% quercetin and 39.36% kaempferol). The medicinal properties of the major components of these phytochemical families that were detected in the aqueous extract of the leaves were discussed herein and proposed to be explored for their potential health benefits. The great number of potentially active biomolecules and their multifunctional properties make Talinum triangulare a ready source of health‐promoting substances.

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“…Lack of any balance between ROS synthesis and antioxidants is referred to as oxidative stress 7 . Inflammatory factors such as NF‐κβ and its associated genes, namely, inducible nitric oxide synthase (iNOS) and COX‐2, can be triggered by oxidative stress, which results in the aging process caused by the pervasive inflammation‐related responses 8 . By scavenging free radicals such as hydroxyl radicals and superoxide anions, antioxidants can reduce the degree of damage caused by ROS, thus helping to prevent organisms' aging 4 .…”

Section: Introductionmentioning

confidence: 99%

Malvidin prevents lipopolysaccharide‐induced oxidative stress and inflammation in human peripheral blood mononuclear cells

et al. 2020

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It is indicated that malvidin has anti‐inflammatory and antioxidant effects on various cells, although the function of malvidin in preventing inflammatory reactions caused by lipopolysaccharide (LPS) in peripheral blood mononuclear cells (PBMCs) is still not known. The objective of this study was to examine the impact of malvidin on inflammatory responses and oxidative stress in PBMCs as caused by LPS. The present findings showed that LPS significantly increased the expression of IL‐6, TNF‐α, IL‐1β, and COX‐2 mRNA and protein release from PBMCs 22 hr after treatments. It was also revealed that increased levels in cytokine expression coincided with increased phosphorylation of JNK, P65‐NF‐κB, and IKKα/IKKβ. Also, the expression of IL‐6, TNF‐α, IL‐1β, and COX‐2 mRNA induced by LPS as well as secretion of protein in PBMC has been significantly decreased by pretreatment of malvidin. Importantly, pretreatment of the cells with malvidin completely abrogated the phosphorylation of P65‐NF‐κB, JNK, and IKKα/IKKβ in LPS treated cells. Malvidin protection against LPS‐induced inflammation was coupled with a decline in the levels of nitric oxide metabolite and malondialdehyde, along with an increase in the ferric reducing antioxidant power, total thiol activity, and also superoxide dismutase and glutathione peroxidase activity. In accordance with this finding, malvidin may represent a promising therapeutic agent for the prevention of inflammation in PBMCs.

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Malvidin Protects WI-38 Human Fibroblast Cells Against Stress-induced Premature Senescence

Cited by 20 publications

References 46 publications

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

Bioactive phytochemicals in an aqueous extract of the leaves of Talinum triangulare

Malvidin prevents lipopolysaccharide‐induced oxidative stress and inflammation in human peripheral blood mononuclear cells

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