Malondialdehyde: A Possible Marker of Ageing

Abstract

Background: Numerous recent studies have suggested that oxidative damage may be important in the ageing process, and lipid peroxidation is an important biological consequence of oxidative cellular damage. Objective: The aim of this work was to analyze the activities of the two protective enzymes, superoxide dismutase (SOD) and catalase (CAT), and the levels of malondialdehyde (MDA) to examine the relationship between the ageing process and defence antioxidant and lipid peroxidation. Method: SOD activity was measured in red blood cells using the Minami and Yoshikawa method; CAT activity was measured in hemolysates by the Aebi method, and MDA levels were measured in erythrocytes by high-performance liquid chromatography. Results: SOD activity shows statistically significant differences between newborns and the rest of the sample (ANOVA p < 0.001; Student-Newman-Keuls test p < 0.001). CAT activity did not show significant differences between the age groups. We observed statistically significant differences in MDA levels between the different groups (ANOVA p < 0.001; Student-Newman-Keuls test p < 0.05). In the regression analysis and rectilinear/curvilinear adjustment compared to age, SOD and CAT showed coefficients close to zero (SOD linear = 0.16; SOD exponential = 0.15; CAT linear = 0.056; CAT exponential = 0.068), indicating in that way their independence from age. Only MDA obtained a regression coefficient superior to 0.75 (p < 0.05). The best adjustment was reached through an exponential expression, giving the following parametric relation: MDA = 103.117e^0.0021.AGE. No statistically significant variation in SOD and CAT activity and MDA levels, related to sex could be demonstrated. Conclusions: Our data show that old age is associated with an increase in systemic oxidative stress.

Relationship between oxidative and occupational stress and aging in nurses of an intensive care unit

“…These results agree with those obtained in the Spanish population at large (De la Torre et al 1990;Casado and López-Fernández 2003;Gil et al 2002) and in the emergency services (Casado et al 2005(Casado et al , 2006. We found higher SOD and CAT activities in nurses than in the control group, but significant differences were obtained in SOD activity only between the group of nurses and the control group for ages from 30 to 39 years.…”

“…These results agree with those obtained in the Spanish population (Gil et al 2002). As can be seen in Table 2, MDA levels increase with age in both the control group and in intensive care unit nurses.…”

“…MDA is the most abundant individual aldehyde resulting from lipid peroxidation; consequently, this aldehyde can be considered as a marker for lipid peroxidation (Draper et al 1988). In this way, elevated levels of MDA are indicative of high oxidative stress (Ambrosio and Flaherty 1991) and aging (Gil et al 2002).…”

Ameliorative Effect of Lycopene on Lipid Peroxidation and Certain Antioxidant Enzymes in Diabetic Patients

“…A significant increase (P<0.001, Figure 1) in XOD activity in diabetic patients could produce a burst of free radicals. Once O 2 -radical produced then H 2 O 2 and .OH are continuously produced by Haber-Weiss reaction and /or Fenton type reaction [23]. Oxygen radicals might cause the lipid peroxidation of biomembrane through a chain reaction.…”

Food supplementation among HIV-infected adults in Sub-Saharan Africa: impact on treatment adherence and weight gain

“…The extent of oxidative damage can be determined by measuring biochemical parameters such as glutathione (GSH), glutathione peroxidase, malondialdehyde or total antioxidant status (19)(20)(21) . The GSH has been shown to be of importance in immune function and restoration of CD4T lymphocyte levels in HIV infection (22)(23)(24) .…”