Malignant mast cell tumor of the thymus in an Royal College of Surgeons (RCS) rat

Terayama, Yui; Matsuura, Tetsuro; Ozaki, Kiyokazu

doi:10.1293/tox.2016-0044

Cited by 7 publications

(5 citation statements)

References 13 publications

(20 reference statements)

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“…82 Mast cell neoplasms are extremely rare and only isolated cases have been reported in rats. 99 In the NTP 2-year bioassays from 1982 to the present time containing 41 238 male and 43 612 female rats, only 4 instances of mast cell tumor have been reported in males and females (2 per sex). See “Incidences of Mast Cell Tumor and Histiocytic Sarcoma in Rats in NTP Studies” for more detailed data: NTP Incidence Data.…”

Section: Granularity Matters For the Diagnosis Of Neoplasmsmentioning

confidence: 79%

Proceedings of the 2019 National Toxicology Program Satellite Symposium

et al. 2019

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The 2019 annual National Toxicology Program Satellite Symposium, entitled “Pathology Potpourri,” was held in Raleigh, North Carolina, at the Society of Toxicologic Pathology’s 38th annual meeting. The goal of this symposium was to present and discuss challenging diagnostic pathology and/or nomenclature issues. This article presents summaries of the speakers’ talks along with select images that were used by the audience for voting and discussion. Various lesions and topics covered during the symposium included aging mouse lesions from various strains, as well as the following lesions from various rat strains: rete testis sperm granuloma/fibrosis, ovarian cystadenocarcinoma, retro-orbital schwannoma, periductal cholangiofibrosis of the liver and pancreas, pars distalis hypertrophy, chronic progressive nephropathy, and renal tubule regeneration. Other cases included polyovular follicles in young beagle dogs and a fungal blood smear contaminant. One series of cases challenged the audience to consider how immunohistochemistry may improve the diagnosis of some tumors. Interesting retinal lesions from a rhesus macaque emphasized the difficulty in determining the etiology of any particular retinal lesion due to the retina’s similar response to vascular injury. Finally, a series of lesions from the International Harmonization of Nomenclature and Diagnostic Criteria Non-Rodent Fish Working Group were presented.

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Section: Granularity Matters For the Diagnosis Of Neoplasmsmentioning

confidence: 79%

Proceedings of the 2019 National Toxicology Program Satellite Symposium

et al. 2019

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“…Number of animals examined for immunohistochemical analysis including normal thymuses, hyperplastic lesions, and thymomas are shown in Table1. The paraffin sections were analyzed immunohistochemically using antibodies against CK18 (Ks18.04, Mouse monoclonal, PROGEN Biotechnik, GmbH, Heidelberg, Germany) for cortical epithelium, 7 CK14 (EPR17350, Rabbit monoclonal; Abcam, Cambridge, MA, USA) for medullary epithelium, 9 and CD20 (M-20, Goat polyclonal; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) for B lymphocytes. Secondary antibody used the polymer kit (HISTOFINE Simple Stain Mouse MAX-PO; Nichirei Bioscience, Inc., Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…7,8 Additionally, Terayama et al reported that medullary thymic epithelial cells in the normal thymus of a Royal College of Surgeons rat are positive for CK14. 9 Brelińska et al reported that CK16 positive for thymic epithelial cells in the outer region of medulla of thymus in Wistar rats. 10 We focused on the differences in CK expression in the thymic epithelium of the cortex and medulla, investigated the type of thymic epithelium present in the cortex-like LyR, MD areas, and pale-staining cell foci observed in thymomas, and compared the structure of thymomas with the normal thymic cortex and medulla.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Characteristics of Thymomas and Hyperplastic Lesions in Wistar Hannover Rats

Tomonari¹,

Sato²,

Yamada³

et al. 2020

Toxicol Pathol

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Previously, we investigated the higher incidence of hyperplastic lesions and thymomas and histopathological resemblance of cortex–medullary structures between thymomas and normal thymuses in Wistar Hannover (WH) rats. Thymomas had pale-staining cell foci (PA) similar to medulla but without lymphocytes. Here, we focused on the differences in cytokeratin (CK) expression in the thymic epithelia of the cortex and medulla and compared the structures of thymomas and normal thymuses. Thymomas, hyperplastic lesions, and normal thymuses obtained from background studies of WH rats were stained with antibodies against CK14, CK18, and CD20. In normal thymuses, the epithelial cells were positive for CK14 in the medulla and subcapsular area and for CK18 in the cortex, B-cells were positive for CD20 in the medulla. In thymomas, the epithelial cells were positive for CK14 in the medullary differentiation (MD) areas and for CK18 in the cortex-like lymphocyte rich and PA, and B-cells were positive for CD20 in the MD areas.

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“…They reported that the granules partially showed a filament-like structure. In feline globule leukocyte tumor 14 or rat mast cell tumor 15 , however, granules contained nonstructural and homogenous materials. It was also reported that the characteristic feature of normal intestinal globule leukocytes in adult rats was the presence of paracrystalline inclusions in the granules 16 .…”

mentioning

confidence: 95%

“…Globule leukocytes of the interepithelium of the gastrointestinal mucosa in rodents are positive for esterase 5 , 6 , mast cell protease 5 , 8 , 9 , 10 , granzyme B 9 , and c-kid 9 . Mast cells are also positive for esterase 5 , mast cell protease 5 , 9 , 15 , granzyme B 9 , and c-kid 9 , 15 , 17 . Granzyme B is a cytotoxic lymphocyte-associated protease in cytotoxic T cells or natural killer (NK) cells, although previous reports suggest that mast cells produce and release granzyme B upon activation 18 , 19 .…”

mentioning

confidence: 99%

Spontaneous globule leukocyte tumor accompanied by inflammatory cells in a Wistar Hannover rat

Sato¹,

Tomonari²,

Doi³

et al. 2019

J Toxicol Pathol

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We encountered hematolymphoid neoplastic lesions in the form of many nodules in the spleen and liver in a 110-week-old male Wistar Hannover rat (Crl:WI (Han)). The lesions contained atypical proliferative cells, eosinophils, lymphocytes, and macrophages. The proliferative cells comprised various atypical cell types with or without cytoplasmic eosinophilic granules. The granules were positively stained using periodic acid-Schiff and elastase stains, were bluish purple using phosphotungstic acid and hematoxylin, and showed no metachromasia using toluidine blue. In immunohistochemical staining, the proliferative cells with or without granules were positive for granzyme B, rat mast cell protease II, and Ki67. Electron microscopic examination revealed that single to multiple high-density granules of variable size were covered by a membrane. These findings led to a diagnosis of globule leukocyte tumor. The accompaniment of this tumor by inflammatory cells is likely evoked by mast cell-like active mediators contained in the granules of the globule leukocytes.

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Malignant mast cell tumor of the thymus in an Royal College of Surgeons (RCS) rat

Cited by 7 publications

References 13 publications

Proceedings of the 2019 National Toxicology Program Satellite Symposium

Proceedings of the 2019 National Toxicology Program Satellite Symposium

Immunohistochemical Characteristics of Thymomas and Hyperplastic Lesions in Wistar Hannover Rats

Spontaneous globule leukocyte tumor accompanied by inflammatory cells in a Wistar Hannover rat

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