Malignant Brain Tumours Associated With Adenylate Kinase in Cerebrospinal Fluid

Ronquist, Gunnar; Ericsson, Per; Frithz, Göran; Hugosson, R.

doi:10.1016/s0140-6736(77)91320-4

Cited by 31 publications

(12 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…9], This is of particular importance since CSF is a deleterious suspending me dium for blood corpuscles due to an en hanced breakdown of intracellular ATP even at room temperature in this medium while the harmful effect is insignificant for hours at 4 °C. Therefore, it is necessary to sample and handle the biological material under defined conditions [8,9], A corresponding descrip tion was not given by those who found ele vated levels of endogenous AK in CSF of normal individuals [5,10]. Another influen tial factor is of course the performance of the bioassay; e.g.. dithioerythritol as a thiol group reactant was not included in the origi nal method [9] although it was used later to optimize the reaction [8],…”

Section: Discussionmentioning

confidence: 99%

“…It is also present in C'SF of patients with malignant brain tu mors [9], The concomitant appearance of glutathione usually indicated a cerebral dam age of a more profound and extensive nature [7,8,11]. Taking these observations into account the corollary is that the determina tion of AK in CSF, sometimes in combina tion with glutathione, can be used under proper conditions as a simple discriminative aid in ruling out an organic brain lesion in the patient in all cases of negative AK results [1,[7][8][9], To extend the working hypothesis fur ther, repeated examinations of AK and gluta thione in CSF during the course of a brain lesion would reveal different curve profiles depending on the nature of the underlying disease. A malignant brain tumor would ac cordingly reveal constant high values, since that type of lesion will not spontaneously go into regress.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Perspectives of Adenylate Kinase Activity and Glutathione Concentration in Cerebrospinal Fluid of Patients with Ischemic and Neoplastic Brain Lesions

Frithz¹,

Ronquist²,

Hugosson³

1982

Eur Neurol

View full text Add to dashboard Cite

Adenylate kinase (AK) activity and glutathione concentration were measured in cerebrospinal fluid (CSF) on three different occasions after the ictus in 16 patients with cerebral infarction. All patients displayed an unambiguous AK activity in their CSF within 48 h after the ictus while glutathione was present in 10 of the 16 patients. The mean AK activity among these patients was reduced by about 60% after 1 week and an additional smaller reduction took place after 3–4 weeks. The glutathione concentration in CSF of these patients showed a similar curve profile with time. The 9 patients who showed clinical signs of improvement had significantly lower values on all occasions for both AK and glutathione than those who did not improve or deteriorated. A corresponding reduction in AK activity with time was not observed in 3 patients with cerebral malignancies. The possibility was excluded that the enhanced AK activity in CSF was the result of an increased transfer of the enzyme from the plasma compartment. Instead a causal relationship was suggested between the appearance of AK in CSF and a disturbed brain cell metabolism. Repeated analyses revealed different curve profiles for ischemic and neoplastic brain lesions. The importance of sampling and handling the CSF material under standardized conditions is emphasized.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Perspectives of Adenylate Kinase Activity and Glutathione Concentration in Cerebrospinal Fluid of Patients with Ischemic and Neoplastic Brain Lesions

Frithz¹,

Ronquist²,

Hugosson³

1982

Eur Neurol

View full text Add to dashboard Cite

show abstract

“…Recently, an enzyme, adenylate kinase (ATP: AMP phosphotransferase, EC 2.7.4.3), occurring in cerebral tissue but normally not present in CSF in adults (6), was demonstrated to be a sensitive marker of even small and most probably reversible ischaemic changes in brain tissue (3). Adenylate kinase (AK) catalyzes the dismutation reaction of two molecules of adenosine diphosphate (ADP) into one mole cule of adenosine monophosphate (AMP) and one molecule of adenosine triphosphate (ATP).…”

Section: Introductionmentioning

confidence: 99%

Adenylate Kinase Activity and Glutathione Concentration of Cerebrospinal Fluid in Different Neurological Disorders

Ronquist¹,

Frithz²

1979

Eur Neurol

View full text Add to dashboard Cite

Adenylate kinase activity and glutathione concentration were measured in cerebrospinal fluid (CSF) of 64 consecutive patients admitted for various neurological disorders. These two analyses were performed in addition to conventional examination of the CSF. Neurological symptoms most probably connected in some cases with no and in others with only subtle changes in the central nervous system were linked to no or only moderate activities of adenylate kinase together with no glutathione. 1 patient with meningioma had no adenylate kinase activity at all while 3 patients with malignant brain tumours showed clear activities similar to 3 patients with well established diagnoses of multiple sclerosis. On the contrary, glutathione was absent in CSF of the patients with brain tumours and multiple sclerosis. Various cerebrovascular diseases involving larger areas of the brain tissue resulted in clear adenylate kinase activities in CSF either alone as in 11 of the patients with cerebral infarction or in combination with the appearance of also glutathione as in the remaining 7 patients with cerebral infarction as well as in the 14 patients studied with hemorrhages of the brain.

show abstract

“…Further, Ronquist et al [12], and Ronquist and Frithz [13] reported that AK was consistently found in the CSF of patients with malignant brain tumors and cerebral infarction. However, Getaz et al [14] claimed that there was no clear correlation between the presence of disease in central nervous system (CNS) and enzyme activity.…”

Section: Resultsmentioning

confidence: 96%

Serum adenylate kinase activity and isoenzyme in acute cerebral vascular disease.

Endo

Iwata

Kuzuya

1989

J. Clin. Biochem. Nutr.

View full text Add to dashboard Cite

SummarySerum adenylate kinase (AK) activity was measured in cerebral vascular diseases and the AK isoenzyme pattern was studied in the patients suffering from cerebral infarction, cerebral hemorrhage, myocardial infarction, and polymyositis. There was a significant difference in AK activity between the control subjects and patients with acute cerebral infarction. Serum AK activity showed a peak on the 2nd day to the 4th day after the stroke attack, maintained at high level for about one week, and decreased gradually thereafter. There were three isoenzymes (AK-1, AK-2, AK-3) of AK, and we found that the AK isoenzyme patterns were different in different disease. The AK isoenzyme patterns in patients with cerebral infarction and cerebral hemorrhage were different from those of control subjects. The AK-3 isoenzyme was dominant in the control subjects. The proportion of AK-1 isoenzyme was increased in cerebral infarction and cerebral hemorrhage compared with that for the control subjects, and the proportion of AK-2 isoenzyme was elevated in acute myocardial infarction and polymyositis. These results suggest that serum AK is a sensitive indicator of cerebral infarction and a useful marker to follow the course of patient recovery. Moreover, the analysis of AK isoenzymes is useful in the diagnosis of cerebral vascular diseases.Key Words: adenylate kinase, isoenzyme, cerebral vascular disease There are many reports concerning elevated creatine phosphokinase (CPK) activity and CPK isoenzymes in acute cerebral vascular diseases (CVD) [1][2][3]. Previously, we studied CPK activity and CPK isoenzymes in the early stage of cerebral infarction, and found that the CPK levels remained within the normal limit in many cases and the band of CPK-BB was not elevated. On the other hand,

show abstract

Malignant Brain Tumours Associated With Adenylate Kinase in Cerebrospinal Fluid

Cited by 31 publications

References 12 publications

Perspectives of Adenylate Kinase Activity and Glutathione Concentration in Cerebrospinal Fluid of Patients with Ischemic and Neoplastic Brain Lesions

Perspectives of Adenylate Kinase Activity and Glutathione Concentration in Cerebrospinal Fluid of Patients with Ischemic and Neoplastic Brain Lesions

Adenylate Kinase Activity and Glutathione Concentration of Cerebrospinal Fluid in Different Neurological Disorders

Serum adenylate kinase activity and isoenzyme in acute cerebral vascular disease.

Contact Info

Product

Resources

About