Maleic anhydride proton sponge as a novel MALDI matrix for the visualization of small molecules (&lt;250 m/z) in brain tumors by routine MALDI ToF imaging mass spectrometry

Giampà, Marco; Lissel, Manfred; Patschkowski, Thomas; Fuchser, Jens; Hans, Volkmar; Gembruch, Oliver; Bednarz, Hanna; Niehaus, Karsten

doi:10.1039/c6cc02387h

Cited by 34 publications

(49 citation statements)

References 34 publications

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“…The use of classical matrices for the MALDI‐MSI detection of low molecular weight compounds leads to strong background signals in the low mass range of the spectrum due to the ionization of the matrix, related clustering processes, and because of susceptibility to in‐source fragmentation of small molecules . The MAPS matrix is suitable for the MALDI‐MSI analysis of small molecules lower than 250 Da . In the study of Giampà et al, it is shown by scanning electron microscope (SEM) images that MAPS matrix produces a thin film when applied on tissues, rather than a crystalline structure as, eg, in case of DHB .…”

Section: Discussionmentioning

confidence: 99%

“…The MAPS matrix is suitable for the MALDI‐MSI analysis of small molecules lower than 250 Da . In the study of Giampà et al, it is shown by scanning electron microscope (SEM) images that MAPS matrix produces a thin film when applied on tissues, rather than a crystalline structure as, eg, in case of DHB . Apparently, the lack of visible crystals does not hinder the adequate ionization of metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…19 In the study of Giampà et al, it is shown by scanning electron microscope (SEM) images that MAPS matrix produces a thin film when applied on tissues, rather than a crystalline structure as, eg, in case of DHB. 19 Apparently, the lack of visible crystals does not hinder the adequate ionization of metabolites. In the contrary, because of this fact, there are no limitations regarding the matrix crystal size or crystal distributions, which enables novel high-resolution MALDIbased imaging methods.…”

Section: Discussionmentioning

confidence: 99%

“…The matrix maleic anhydride proton sponge (MAPS) was synthesized according to previously reported procedures, and solutions for immersion were prepared at a concentration of 5 mg/mL by dissolving in chloroform, dichloromethane, or toluene.…”

Section: Methodsmentioning

confidence: 99%

“…The matrix maleic anhydride proton sponge (MAPS) was synthesized according to previously reported procedures, 19,20 and solutions for immersion were prepared at a concentration of 5 mg/mL by dissolving in chloroform, dichloromethane, or toluene. μL/min; pressure, 10 psi; track spacing, 3 mm; velocity, 600 mm/ min; and nozzle temperature, 30 C. After, matrix application slides were placed in a desiccator until MALDI-MSI analysis.…”

Section: Maps Synthesismentioning

confidence: 99%

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Immersion by rotation‐based application of the matrix for fast and reproducible sample preparations and robust results in mass spectrometry imaging

Schäfermann

Kliewer

Lösch³

et al. 2020

J Mass Spectrom

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Automated matrix deposition for matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is crucial for producing reproducible analyte ion signals. Here we report an innovative method employing an automated immersion apparatus, which enables a robust matrix deposition within 5 minutes and with scalable throughput by using MAPS matrix and non‐polar solvents. MSI results received from mouse heart and rat brain tissues were qualitatively similar to those from nozzle sprayed samples with respect to peak number and quality of the ion images. Overall, the immersion‐method enables a fast and careful matrix deposition and has the future potential for implementation in clinical tissue diagnostics.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Maps Synthesismentioning

confidence: 99%

See 3 more Smart Citations

Immersion by rotation‐based application of the matrix for fast and reproducible sample preparations and robust results in mass spectrometry imaging

Schäfermann

Kliewer

Lösch³

et al. 2020

J Mass Spectrom

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MALDI Matrices for the Analysis of Low Molecular Weight Compounds: Rational Design, Challenges and Perspectives

Qiao

Lissel

2021

Chemistry An Asian Journal

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The analysis of low molecular weight (LMW) compounds is of great interest to detect small pharmaceutical drugs rapidly and sensitively, or to trace and understand metabolic pathways. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) plays a central role in the analysis of high molecular weight (bio)molecules. However, its application for LMW compounds is restricted by spectral interferences in the low m/z region, which are produced by conventional organic matrices. Several strategies regarding sample preparation have been investigated to overcome this problem. A different rationale is centred on developing new matrices which not only meet the fundamental requirements of good absorption and high ionization efficiency, but are also vacuum stable and "MALDI silent", i. e., do not give matrix-related signals in the LMW area. This review gives an overview on the rational design strategies used to develop matrix systems for the analysis of LMW compounds, focusing on (i) the modification of well-known matrices, (ii) the search for high molecular weight matrices, (iii) the development of binary, hybrid and nanomaterial-based matrices, (iv) the advance of reactive matrices and (v) the progress made regarding matrices for negative or dual polarity mode.

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Mass spectrometry imaging for direct visualization of components in plants tissues

Han

Sheng

et al. 2021

J of Separation Science

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Mass spectrometry is considered the most informative technique for components identification and has been widely adopted in plant sciences. However, the spatial distribution of compounds in the plant, which is vital for the exploration of plant physiological mechanisms, is missed in MS analysis. In recent years, mass spectrometry imaging has brought a great breakthrough in plant analysis because it can determine both the molecular compositions and spatial distributions, which is conducive to understand functions and regulation pathways of specific components in plants. Mass spectrometry imaging analysis of plant tissue is toward high sensitivity, high spatial resolution, and even single-cell analysis. Despite many challenges and technical barriers, such as difficulties of sample pretreatment caused by morphological diversity of plant tissues, obstacles for high spatial resolution imaging, and so on, lots of researches have contributed to remarkable progress, including improvement in tissue preparation, matrix innovation, and ionization mode development. This review focuses on the advances of mass spectrometry imaging analysis of plants in the last 5 years, including commonly used ionization techniques, technical advances, and recent applications of mass spectrometry imaging in plants.

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Maleic anhydride proton sponge as a novel MALDI matrix for the visualization of small molecules (<250 m/z) in brain tumors by routine MALDI ToF imaging mass spectrometry

Cited by 34 publications

References 34 publications

Immersion by rotation‐based application of the matrix for fast and reproducible sample preparations and robust results in mass spectrometry imaging

Immersion by rotation‐based application of the matrix for fast and reproducible sample preparations and robust results in mass spectrometry imaging

MALDI Matrices for the Analysis of Low Molecular Weight Compounds: Rational Design, Challenges and Perspectives

Mass spectrometry imaging for direct visualization of components in plants tissues

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