Male sterility and its importance in breeding heterosis varieties

Kley, F.K. van der

doi:10.1007/bf00029958

Cited by 24 publications

(6 citation statements)

References 3 publications

(5 reference statements)

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“…In Arabidopsis, like in most crop species, the genomes of cytoplasmic organelles are inherited uniparentally from the mother via the egg cell. Despite the importance of maternal cytoplasm in breeding [28][29][30] , its effect on phenotype is not easy to ascertain because producing different nuclear versus cytoplasmic combinations normally requires hybridization followed by multiple generations of backcrossing. Genome elimination during haploid induction provides a rapid method for producing cytoplasm-swapped genotypes because the maternal cytoplasm is maintained while the entire maternal nuclear HI genome is eliminated 16 .…”

Section: Resultsmentioning

confidence: 99%

A haploid genetics toolbox for Arabidopsis thaliana

et al. 2014

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Genetic analysis in haploids provides unconventional yet powerful advantages not available in diploid organisms. In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wild-type strain to a transgenic strain with altered centromeres. Here we report the development of an improved haploid inducer (HI) strain, SeedGFP-HI, that aids selection of haploid seeds prior to germination. We also show that haploids can be used as a tool to accelerate a variety of genetic analyses, specifically pyramiding multiple mutant combinations, forward mutagenesis screens, scaling down a tetraploid to lower ploidy levels and swapping of nuclear and cytoplasmic genomes. Furthermore, the A. thaliana HI can be used to produce haploids from a related species A. suecica and generate homozygous mutant plants from strong maternal gametophyte lethal alleles, which is not possible via conventional diploid genetics. Taken together, our results demonstrate the utility and power of haploid genetics in A. thaliana.

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Section: Resultsmentioning

confidence: 99%

A haploid genetics toolbox for Arabidopsis thaliana

et al. 2014

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“…These results laid a solid foundation for further research on the molecular mechanism of wheat male sterility.which is one of the key ways to improve wheat yield [6]. Male sterility permits the production of hybrids on a commercial scale, relying on heterosis in crops, and can greatly increase selection efficiency of the yield [7]. In-depth understanding of the molecular mechanism of male sterility can help us to use it more effectively.Plant male sterility was first reported in the 18th century [8].…”

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confidence: 92%

“…which is one of the key ways to improve wheat yield [6]. Male sterility permits the production of hybrids on a commercial scale, relying on heterosis in crops, and can greatly increase selection efficiency of the yield [7]. In-depth understanding of the molecular mechanism of male sterility can help us to use it more effectively.…”

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confidence: 99%

The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

Zhang

et al. 2019

IJMS

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Male sterility is a valuable trait for genetic research and production application of wheat (Triticum aestivum L.). NWMS1, a novel typical genic male sterility mutant, was obtained from Shengnong 1, mutagenized with ethyl methane sulfonate (EMS). Microstructure and ultrastructure observations of the anthers and microspores indicated that the pollen abortion of NWMS1 started at the early uninucleate microspore stage. Pollen grain collapse, plasmolysis, and absent starch grains were the three typical characteristics of the abnormal microspores. The anther transcriptomes of NWMS1 and its wild type Shengnong 1 were compared at the early anther development stage, pollen mother cell meiotic stage, and binucleate microspore stage. Several biological pathways clearly involved in abnormal anther development were identified, including protein processing in endoplasmic reticulum, starch and sucrose metabolism, lipid metabolism, and plant hormone signal transduction. There were 20 key genes involved in the abnormal anther development, screened out by weighted gene co-expression network analysis (WGCNA), including SKP1B, BIP5, KCS11, ADH3, BGLU6, and TIFY10B. The results indicated that the defect in starch and sucrose metabolism was the most important factor causing male sterility in NWMS1. Based on the experimental data, a primary molecular regulation model of abnormal anther and pollen developments in mutant NWMS1 was established. These results laid a solid foundation for further research on the molecular mechanism of wheat male sterility.which is one of the key ways to improve wheat yield [6]. Male sterility permits the production of hybrids on a commercial scale, relying on heterosis in crops, and can greatly increase selection efficiency of the yield [7]. In-depth understanding of the molecular mechanism of male sterility can help us to use it more effectively.Plant male sterility was first reported in the 18th century [8]. A large number of plant male sterility mutants have been reported over the past few decades. Male sterility includes cytoplasmic male sterility (CMS) controlled by mitochondrial genes coupled with nuclear genes, and genic male sterility (GMS) controlled by nuclear genes alone [9][10][11]. The pistils of the cytoplasmic male sterile lines and genic male sterile lines are normal, but their stamens are abnormal and cannot pollinate normally, which is due to stamen degeneration, pollen abortion, or functional sterility. GMS occurs in 216 species and 17 species crosses, while CMS occurs in more than 150 species and 271 species crosses [11][12][13]. Plant male sterility lines have been widely studied and applied successfully in many crops, such as maize (Zea mays), rice (Oryza sativa), soybean (Glycine max), and barley (Hordeum vulgare). Several wheat CMS lines have been studied and applied in production; however, commercial application is limited [14][15][16]. Possible applications of GMS in plant breeding have been reviewed and discussed for at least 30 years [17]. Up to now, only five GMS genes have been...

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“…It can be triggered by multiple factors including adverse growth conditions such as temperature (Endo et al, 2009), diseases, inheritance, mutations or chemical agents (McRae, 1985;Budar and Pelletier, 2001). The deliberate elimination of the male gamete using CHAs has been established as a potentially viable approach in commercial hybrid seed production (Van Der Kley, 1954;Tu and Banga, 1998). It would solve many problems, such as the elimination of tedious hand emasculation, allowing for combinations of suitable parents, eliminating the need for expensive, complex and limiting cytoplasmic male sterility systems, increasing crossing choices in plant breeding and simplifying genetic analysis of self-pollinating crops.…”

Section: Introductionmentioning

confidence: 99%

The efficacy of four gametocides for induction of pollen sterility in Eragrostis tef (Zucc.) Trotter

Ghebrehiwot¹,

Burgdorf²,

Shimelis³

et al. 2015

Afr. J. Biotechnol.

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Effective chemical hybridizing agents (CHAs) or male gametocides enhance cross pollination in plant breeding and genetic analysis of traits. The present study examined the efficacy and optimum concentration of four CHAs, namely: 2-chloroethyl phosphonic acid (Ethrel), ethyl 4'-fluorooxanilate (E4FO), 2, 4-dichlorphenoxy acetic acid (2, 4-D) and Promalin ® (1.8% GA 47 -gibberellins A 4 +A 7 and 1.8% 6-BA-benzyladenine) on pollen sterility and seeding of a tef line (DZ-01-3186). Seed-derived and individually grown tef plants were treated with foliar applications of the four CHAs (at four levels each) sprayed once at the early booting stage, and bagged to control cross pollination. Female fertility was assessed by recording seed set following controlled pollinations. Although all the CHAs caused some pollen sterility, their efficacy levels varied from 9.77 to 99.50% in treated plants compared to the control (6.68 +/-1.04%). Pollen sterility increased with increasing CHA concentration. Near-complete pollen sterility (99.50 ± 0.50%) was achieved by the application of E4FO at rates of 1500 to 3000 ppm, and Ethrel at 5000 ppm. All the CHAs significantly reduced seed yield, with E4FO, Ethrel and Promalin ® at 5000 ppm causing the highest reduction, essentially yielding no seed. In the period following the application of the CHAs, plants treated with E4FO (1000 -1500 ppm) exhibited stigmas that remained fertile. Hence, it is recommended that E4FO (at 1000 -1500 ppm) can be used as a chemical emasculation agent for tef, with the least phytotoxicity and the highest female fertility.

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Male sterility and its importance in breeding heterosis varieties

Cited by 24 publications

References 3 publications

A haploid genetics toolbox for Arabidopsis thaliana

A haploid genetics toolbox for Arabidopsis thaliana

The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

The efficacy of four gametocides for induction of pollen sterility in Eragrostis tef (Zucc.) Trotter

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