Male rat exposure to low dose of di(2-ethylhexyl) phthalate during pre-pubertal, pubertal and post-pubertal periods: Impact on sperm count, gonad histology and testosterone secretion

Oudir, Malha; Chader, Henni; Bouzid, B.; Bendisari, Kheira; Latreche, Batoul; Boudalia, Sofiane; Iguer-Ouada, Mokrane

doi:10.1016/j.reprotox.2017.11.004

Cited by 36 publications

(24 citation statements)

References 32 publications

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“…Alteration of cholesterol transport and steroidogenic enzymes in MLTC-1 cells may be involved in the biphasic effects of PFOA on androgen production. Our findings agree with the hypothesis that low-dose stimulation corresponds to a negative feedback compensation mechanism that counterbalances the endocrine disrupting chemicals-induced inhibition of gene expression of the steroidogenic enzymes [43][44]. In conclusion, our results suggest that PFOA disrupts cholesterol precursor fatty acid transport into mitochondria and then alters cholesterol synthesis.…”

Section: Discussionsupporting

confidence: 91%

Biphasic effects of perfluorooctanoic acid on steroidogenesis in mouse Leydig tumour cells

Tian

Huang

Wang³

et al. 2019

Reproductive Toxicology

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Perfluorooctanoic acid (PFOA) is a persistent organic pollutant, which may possess endocrine disrupting properties. Herein, we investigated the possible mechanism(s) of toxicity and steroidogenesis in mouse Leydig cells. MLTC-1 (mouse Leydig tumour cells) cells were exposed to 0, 50, 100 or 200 µM PFOA for 48 h to ascertain their effects on the nuclear (membrane) receptor responses, steroidogenesis pathway and related regulated gene expression and steroid hormone secretion profiles. Our results reveal that nuclear receptor PXR, SR-B1 and LHR are sensitive to PFOA exposure. PFOA can accumulate in mitochondria and alter cholesterol precursor (fatty acid) mitochondrial transport process-related gene expression and thus inhibit steroid hormone precursor (cholesterol) production. In particular, PFOA exhibits biphasic effects on testosterone and progesterone production at differing levels of exposure. These findings indicate the potential endocrine-related effects of PFOA on steroid hormone secretion in Leydig cells and point to a novel disruption model.

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Section: Discussionsupporting

confidence: 91%

Biphasic effects of perfluorooctanoic acid on steroidogenesis in mouse Leydig tumour cells

Tian

Huang

Wang³

et al. 2019

Reproductive Toxicology

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“…Previous study confirmed that AFB1 could increase oxidative stress (OS), malondialdehyde (MDA) levels in testis, deprivation of testicular testosterone synthesis, and tissue cell apoptosis (Palacios et al, 2016;Ren et al, 2012). Earlier research showed that the decline in sperm concentration was closely related to the occurrence of oligospermia, and it was directly associated with the total measurement of testis weights and the percentage of viable Sertoli cells (Oudir et al, 2018). It has also been shown that the administration of AFB1 is associated with degeneration of Leydig cells and a lower spermatogenesis rate (Faridha, Faisal, & Akbarsha, 2006).…”

Section: Discussionmentioning

confidence: 92%

“…This evaluation was analysed within 2-4 min of sperm isolation from the epididymis. Sperm motility percentage was measured as the proportion of motile sperm in the total evaluated sperm (Oudir et al, 2018;Rezvanfar et al, 2013). Sperm concentration was determined using the Neubauer chamber as described by Pant and Srivastava (2003).…”

Section: Assessment Of Sperm Characteristicsmentioning

confidence: 99%

Ameliorative effect of selenium nanoparticles on the structure and function of testis and in vitro embryo development in Aflatoxin B1‐exposed male mice

et al. 2020

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The purpose of the research was to investigate the therapeutic ability of selenium nanoparticles (Se‐NPs) on the aflatoxin B1 (AFB1) toxicity induced in the male reproductive system. For this experiment, the mature male mice were put into four groups. Control (0.5 ml PBS, 60 days; IP, n = 7), Se‐NPs (0.5 µg kg−1 day−1 for 60 days; IP), AFB1 (4.5 mg kg−1 day−1 for 60 days; IP) and AFB1 + Se‐NPs (4.5 mg kg−1 day−1 + 0.5 µg kg−1 day−1 for 60 days; IP). After treatment, the histological structure of testis, serum testosterone level and sperm parameters, including concentration, motility, viability, morphology and DNA fragmentation, were examined. The results demonstrated that the AFB1 destroyed the testicular tissue structure and decreased the sperm concentration, motility, viability and normal morphology significantly. AFB1 also could significantly increase sperm DNA fragmentation and reduce in vitro fertilisation and embryo development compared to the control group (p < .001). Our data show that Se‐NPs could inhibit AFB1‐induced damage to the testis and improve sperm parameters as well as in vitro fertilisation and embryo production in AFB1 exposed male mice. This study revealed that the administration of Se‐NPs could attenuate the testicular injury of AFB1 and improve the male reproductive system function in AFB1 exposed mice.

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“…49 EDCs can have more effects at low doses than that at higher ones, others have different effects at different doses. 12,[50][51][52][53][54][55] The term "low dose" is defined in two ways according to Owens and Chaney, (2005) reported by Bergman and Heindel:…”

Section: Notions Of Edcs Low Dosesmentioning

confidence: 99%

Relationship between endocrine disruptors and obesity with a focus on bisphenol A: a narrative review

et al. 2020

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Introduction: Scientific data suggest that early exposure to endocrine-disrupting chemicals (EDCs) affect -repro, -neuro, -metabolic systems, to which are added other notions such as mixtures, window and duration of exposure, trans-generational effects, and epigenetic mechanisms. Methods: In the present narrative review, we studied the relationship between exposure to EDCs with the appearance and development of obesity. Results: Exposure to EDCs like Bisphenol A during the early stages of development has been shown to lead to weight gain and obesity. EDCs can interfere with endocrine signaling, affect adipocytes differentiation and endocrine function and disrupt metabolic processes, especially if exposure occurs at very low doses, in the mixture, during early development stages for several generations. Conclusion: Exposure to EDCs is positively associated with obesity development. Moreover, the use of integrative approaches which mimicking environmental conditions are necessary and recommended to evaluate EDCs' effects in future studies.

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Male rat exposure to low dose of di(2-ethylhexyl) phthalate during pre-pubertal, pubertal and post-pubertal periods: Impact on sperm count, gonad histology and testosterone secretion

Cited by 36 publications

References 32 publications

Biphasic effects of perfluorooctanoic acid on steroidogenesis in mouse Leydig tumour cells

Biphasic effects of perfluorooctanoic acid on steroidogenesis in mouse Leydig tumour cells

Ameliorative effect of selenium nanoparticles on the structure and function of testis and in vitro embryo development in Aflatoxin B1‐exposed male mice

Relationship between endocrine disruptors and obesity with a focus on bisphenol A: a narrative review

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