Male meiosis and pollen morphology in diploid Indonesian wild bananas and cultivars

Ahmad, Fajarudin; Poerba, Yuyu Suryasari; Kema, Gert H. J.; Jong, Hans de

doi:10.1007/s13237-021-00350-7

Cited by 9 publications

(4 citation statements)

References 41 publications

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“…The high pollen viability for tetraploid bananas is also uncommon since Fortescue and Turner (2004) showed that tetraploids of Indian and Australian origin had pollen viability of 28% and 29%, respectively. The high level of pollen viability of diploid Rejang has similar observations by Fortescue and Turner (2004) that indicated pollen viability of 84% of diploid M. acuminata and confirmed by Ahmad (2021) that demonstrated pollen viability of 77.7% from Rejang diploid. Since the frequency of viable pollen in a viability test indicates the fertility level in bananas (Fortescue and Turner, 2004), this result shows that the diploid natural cultivar of "Pisang Rejang" and its tetraploid and mixoploid derivative is amenable for a breeding parent.…”

Section: Discussionsupporting

confidence: 76%

Male Flower Characteristics of Induced Tetraploid, Mixoploid, and Diploid Banana Musa Acuminata (Aa) Cv. “Pisang Rejang”

MARTANTI¹,

POERBA²,

WITJAKSONO³

et al. 2022

SABRAOJBG

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The banana cultivar “Pisang Rejang” (Musa AA “Pisang Rejang”) is Fusarium wilt resistant and therefore important for banana breeding. Tetraploid (4x) and mixoploid (2x+4x) plants of this cultivar have been induced with oryzalin, and crosses of these two ploidy levels resulted in a triploid hybrid (3x) plant. The availability of these various ploidy levels needs to be accompanied by data on their reproductive biology for their efficient utilization for breeding. This study characterized male flowers concerning qualitative and quantitative morphology, pollen viability, pollen size, nectar volume, nectar Brix index, and acidity level of those different ploidy levels. Observations indicated no differences in qualitative morphological traits, such as, shape and color, but for quantitative morphological traits, such as, size, distinction existed among the different ploidy levels. The triploid plant has huge flower parts in all the quantitative morphological traits, except compound tepal width, followed by the tetraploid, diploid, and mixoploid. Variations also occurred in pollen viability, pollen size, and nectar characters from each level of ploidy. Pollen viability is the highest for the diploid plant, while the tetraploid plant produces bigger pollen than the diploid. The triploid plant produced a low percentage of viable pollen. The study findings indicate that ploidy level affects the flower's quantitative morphological characteristics, pollen viability, and pollen size, but not the flower's qualitative morphological characteristics.

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Section: Discussionsupporting

confidence: 76%

Male Flower Characteristics of Induced Tetraploid, Mixoploid, and Diploid Banana Musa Acuminata (Aa) Cv. “Pisang Rejang”

MARTANTI¹,

POERBA²,

WITJAKSONO³

et al. 2022

SABRAOJBG

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“…Chromosome associations were also investigated in early prophase I cells, specifically at pachytene using DAPI, a blue fluorescing DNA-specific stain, to reveal chromosome details (see Ahmad et al, 2021) and FISH using telomeric probes. At this stage, the telomeres were labelled using rhodamine-labelled synthetic oligonucleotides (5 0 TELO1F-FLUORO-CCC TAA ACC CCT AAA CCC TAA ACC CTA AAC CCT AAA 3 0 and 3 0 TELO1R-RHOD-CCC TAA ACC CTA CCT AAA CCC TAA ACC CTA AAC CCT AAA 5 0 , Life Technologies).…”

Section: Pairing Investigation Using Fish With Centromeric and Telome...mentioning

confidence: 99%

Meiotic abnormalities in sugarcane (Saccharum spp.) and parental species: Evidence for peri‐ and paracentric inversions

Oliveira

Soares

Costa

et al. 2023

Annals of Applied Biology

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The modern cultivars of sugarcane (Saccharum spp.) are highly polyploid and accumulate aneuploidies due to their history of domestication, genetic improvement and interspecific hybrid origin involving the domesticated sweet species Saccharum officinarum (‘noble cane’) and the wild Saccharum spontaneum, both with an evolutionary history of polyploidy. The first hybrids were backcrossed with S. officinarum, and selection from progenies in subsequent generations established the genetic basis of modern cultivars. Saccharum genome complexity has inspired several molecular studies that have elucidated aspects of sugarcane genome constitution, architecture and cytogenetics. Herein, we conducted a comparative analysis of the meiotic behaviour of representatives of the parentals S. officinarum and S. spontaneum, and the commercial variety, SP80‐3280. S. officinarum, an octoploid species, exhibited regular meiotic behaviour. In contrast, S. spontaneum and SP80‐3280 exhibited several abnormalities from metaphase I to the end of division. We reported and typified, for the first time, the occurrence of peri‐ and paracentric inversions. Using in‐situ hybridisation techniques, we were able to determine how pairing association occurred at diakinesis, the origin of lagging chromosomes and, in particular, the mitotic chromosome composition of SP80‐3280. Interestingly, S. spontaneum and recombinant chromosomes showed the most marked tendency to produce laggards in both divisions. Future attempts to advance knowledge on sugarcane genetics and genomics should take meiotic chromosome behaviour information into account.

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“…2022 ). Diploid Musa enabled normal meiotic chromosome behaviour with bivalent chromosome pairs and normal balanced genomic segregation, resulting in fertile pollen ( Damaiyani and Hapsari 2018 ; Ahmad et al . 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Pollen viabilities and gene expression profiles across Musa genomes

Mingmanit,

Boonsrangsom,

Sujipuli

et al. 2023

AoB PLANTS

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Banana (Musa spp.) is a major global economic fruit crop. However, cross-pollination from other Musa cultivars grown in nearby plantations results in seeded fruit that exceeds market demand. This study investigated pollen viability and germination and examined the expression profiles of pollen development-related genes across seven Musa genomes (AA, BB, AAA, BBB, AAB, ABB and ABBB). Twenty-three Musa cultivars were assessed for pollen viability using LAO and TTC staining methods. Results revealed that pollen viability obtained from both methods was significantly different among all the studied cultivars. Cultivars carrying BB (diploid) genomes had higher viability percentages than AA (diploid), AAA, BBB, AAB and ABB (triploid) and ABBB (tetraploid) genomes. Germination of the studied cultivars was also investigated on pollen culture medium, with results showing significant differences between the pollen of each cultivar. The best germinating cultivar was TKM (11.0%), carrying BB genome. Expression profiles of pollen development-related genes by RT-qPCR indicated that both TPD1A and MYB80 genes were highly expressed in triploid Musa genomes but the PTC1 gene showed down-regulated expression, resulting in non-viable pollen. Pollen viability, pollen germination and pollen development-related genes differed across Musa cultivars. This knowledge will be useful for the selection of male parents for Musa cross-breeding programs. Pollen viability should also be considered when planning Musa production to avoid seeded fruit.

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Male meiosis and pollen morphology in diploid Indonesian wild bananas and cultivars

Cited by 9 publications

References 41 publications

Male Flower Characteristics of Induced Tetraploid, Mixoploid, and Diploid Banana Musa Acuminata (Aa) Cv. “Pisang Rejang”

Male Flower Characteristics of Induced Tetraploid, Mixoploid, and Diploid Banana Musa Acuminata (Aa) Cv. “Pisang Rejang”

Meiotic abnormalities in sugarcane (Saccharum spp.) and parental species: Evidence for peri‐ and paracentric inversions

Pollen viabilities and gene expression profiles across Musa genomes

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