MALDI-TOF MS Genotyping of Polymorphisms Related to 1-Carbon Metabolism Using Common and Mass-Modified Terminators

Meyer, Klaus; Fredriksen, Åse; Ueland, Per Magne

doi:10.1373/clinchem.2008.115378

Cited by 13 publications

(10 citation statements)

References 38 publications

(39 reference statements)

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“…Compliance was judged by capsule counts and interviews. Study‐related analyses of circulating B‐vitamin and homocysteine levels and genotyping of the MTHFR (NCBI Entrez Gene 4524) 677C‐‐‐>T polymorphism were performed at Bevital AS, Bergen, Norway, using published methods [25–29].…”

Section: Methodsmentioning

confidence: 99%

Combined analyses and extended follow‐up of two randomized controlled homocysteine‐lowering B‐vitamin trials

Ebbing

Bønaa

Arnesen

et al. 2010

Journal of Internal Medicine

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Section: Methodsmentioning

confidence: 99%

Combined analyses and extended follow‐up of two randomized controlled homocysteine‐lowering B‐vitamin trials

Ebbing

Bønaa

Arnesen

et al. 2010

Journal of Internal Medicine

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“…concentrations of riboflavin, pyridoxal 5ʹ-phosphate, methionine, choline, betaine, dimethylglycine, sarcosine, and creatinine were measured with liquid chromatography-mass spectrometry methods. 21,22 total plasma homocysteine concentrations were measured using an isotope dilution gas chromatographymass spectrometry method. 23 Folate and vitamin B12 concentrations were determined with a microbiological method using Lactobacillus casei and Lactobacillus leichmannii, respectively, and adapted to a microtiter plate format and carried out by a robotic workstation.…”

Section: Blood Sampling and Analysesmentioning

confidence: 99%

“…24,25 in addition, three single nucleotide polymorphisms (BHMT 742g>a, MTR 2756a>g, and MTHFR 677c>t) were determined using MalDi-tOF mass spectrometry. 22 to prevent batch effects, samples were analyzed in matched case-control sets, with the position of the case randomized within each set. the investigators and laboratory staff were blinded to case and control status.…”

Section: Blood Sampling and Analysesmentioning

confidence: 99%

Components of One-carbon Metabolism Other than Folate and Colorectal Cancer Risk

et al. 2016

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Background: Despite extensive study, the role of folate in colorectal cancer remains unclear. research has therefore begun to address the role of other elements of the folate-methionine metabolic cycles. this study investigated factors other than folate involved in one-carbon metabolism, i.e., choline, betaine, dimethylglycine, sarcosine, and methionine and relevant polymorphisms, in relation to the risk of colorectal cancer in a population with low intakes and circulating levels of folate. Methods: this was a prospective case-control study of 613 case subjects and 1,190 matched control subjects nested within the population-based northern Sweden Health and Disease Study. We estimated odds ratios (Or) by conditional logistic regression, and marginal risk differences with weighted maximum likelihood estimation using incidence data from the study cohort. Results: Higher plasma concentrations of methionine and betaine were associated with modest colorectal cancer risk reductions (Or [95% confidence interval {ci}] for highest versus lowest tertile: 0.76 [0.57, 0.99] and 0.72 [0.55, 0.94], respectively). estimated marginal risk differences corresponded to approximately 200 fewer colorectal cancer cases per 100,000 individuals on average. We observed no clear associations between choline, dimethylglycine, or sarcosine and colorectal cancer risk. the inverse association of methionine was modified by plasma folate concentrations (Or [95% ci] for highest/ lowest versus lowest/lowest tertile of plasma methionine/folate concentrations 0.39 [0.24, 0.64], P interaction = 0.06). Conclusions: in this population-based, nested case-control study with a long follow-up time from baseline to diagnosis (median: 8.2 years), higher plasma concentrations of methionine and betaine were associated with lower colorectal cancer risk.See Video abstract at http://links.lww.com/eDe/B83. (Epidemiology 2016;27: 787-796) O ne-carbon metabolism is an intracellular network involving the folate and methionine cycles, in which methyl groups and other one-carbon units are transferred to acceptor molecules ( Figure 1). a central role for one-carbon metabolism in cancer development is biologically plausible, as related nucleotide synthesis and methylation reactions including Dna methylation are vital for genome stability and function.the potential role of one-carbon metabolism in colorectal cancer development has been extensively studied, with most research to date focusing on folate. Diets rich in natural folates are associated with reduced risk of colorectal cancer. 1 However, a dual role of folate has been proposed, both preventing and promoting cancer, depending on the dose and timing of exposure. 2,3 Folate is not a one-carbon unit donor per se, but an enzymatic co-factor important for maintenance of the substrate flux in one-carbon metabolism. it might therefore be expected to stabilize the genome of normal mucosa, protecting against cancer, while accelerating the progression of precancerous or malignant lesions. 2 although evidence from animal...

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“…Plasma concentrations of tHcy, tCys, kynurenine and tryptophan were measured using an isotope dilution gas chromatography–mass spectrometry method (between‐day CV: 2–9%) . Folate and cobalamin concentrations were determined by a microbiological method using Lactobacillus casei and Lactobacillus leichmannii , respectively, adapted to a microtiter plate format and carried out by a robotic workstation (between‐day CV: 5%), MTHFR rs1801133 genotype was determined using MALDI‐TOF mass spectrometry (estimated average error rate of ≤0.1% in duplicated samples) . The investigators and laboratory staff were blinded to case and control status.…”

Section: Methodsmentioning

confidence: 99%

One‐carbon metabolite ratios as functional B‐vitamin markers and in relation to colorectal cancer risk

Gylling

Myte

Ulvik

et al. 2018

Intl Journal of Cancer

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One-carbon metabolism biomarkers are easily measured in plasma, but analyzing them one at a time in relation to disease does not take into account the interdependence of the many factors involved. The relative dynamics of major one-carbon metabolism branches can be assessed by relating the functional B-vitamin marker total homocysteine (tHcy) to transsulfuration (total cysteine) and methylation (creatinine) outputs. We validated the ratios of tHcy to total cysteine (Hcy:Cys), tHcy to creatinine (Hcy:Cre), and tHcy to cysteine to creatinine (Hcy:Cys:Cre) as functional markers of B-vitamin status. We also calculated the associations of these ratios to colorectal cancer (CRC) risk. Furthermore, the relative contribution of potential confounders to the variance of the ratio-based B-vitamin markers was calculated by linear regression in a nested case-control study of 613 CRC cases and 1190 matched controls. Total B-vitamin status was represented by a summary score comprising Z-standardized plasma concentrations of folate, cobalamin, betaine, pyridoxal 5'-phosphate, and riboflavin. Associations with CRC risk were estimated using conditional logistic regression. We found that the ratio-based B-vitamin markers all outperformed tHcy as markers of total B-vitamin status, in both CRC cases and controls. Additionally, associations with CRC risk were similar for the ratio-based B-vitamin markers and total B-vitamin status (approximately 25% lower risk for high versus low B-vitamin status). In conclusion, ratio-based B-vitamin markers were good predictors of total B-vitamin status and displayed similar associations as total B-vitamin status with CRC risk. Since tHcy and creatinine are routinely clinically analyzed, Hcy:Cre could be easily implemented in clinical practice. This article is protected by copyright. All rights reserved.

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MALDI-TOF MS Genotyping of Polymorphisms Related to 1-Carbon Metabolism Using Common and Mass-Modified Terminators

Cited by 13 publications

References 38 publications

Combined analyses and extended follow‐up of two randomized controlled homocysteine‐lowering B‐vitamin trials

Combined analyses and extended follow‐up of two randomized controlled homocysteine‐lowering B‐vitamin trials

Components of One-carbon Metabolism Other than Folate and Colorectal Cancer Risk

One‐carbon metabolite ratios as functional B‐vitamin markers and in relation to colorectal cancer risk

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