MALDI‐TOF MS for Microbial Identification: Years of Experimental Development to an Established Protocol

Kallow, Wibke; Erhard, Marcel; Shah, Haroun N.; Raptakis, E.; Welker, Martin

doi:10.1002/9780470665497.ch12

Cited by 37 publications

(30 citation statements)

References 116 publications

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“…To determine chemotaxonomical markers, a Matrix-Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry (MALDI-TOF MS) analysis of P. entomophila L48 T and its closely related type strains was performed at Anagnosteg GmbH (Germany) [8] as described by Scotta et al [20]. The profile of peaks obtained for each species was analysed and compared using the software BGP database available at the website http://sourceforge.net/projects/bgp.…”

Section: Introductionmentioning

confidence: 99%

Taxonomic characterisation of Pseudomonas strain L48 and formal proposal of Pseudomonas entomophila sp. nov.

Mulet

Gomila

Lemaître

et al. 2012

Systematic and Applied Microbiology

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Section: Introductionmentioning

confidence: 99%

Taxonomic characterisation of Pseudomonas strain L48 and formal proposal of Pseudomonas entomophila sp. nov.

Mulet

Gomila

Lemaître

et al. 2012

Systematic and Applied Microbiology

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“…The resulting mass spectra display peak patterns that have been shown to be phylogenetic markers (Wynne et al 2009). For bacteria -or prokaryotes in general -it has been shown that the peaks recorded in MALDI-TOF mass spectra can be assigned partially to ribosomal proteins (Teramoto 2009;Kallow et al 2010), thus representing biomarkers that can be exploited for taxonomic studies (Kroppenstedt et al 2005) and routine identification (Freiwald, and Sauer 2009).…”

Section: Introductionmentioning

confidence: 99%

Discrimination of freshwater fish species by Matrix-Assisted Laser Desorption/Ionization- Time Of Flight Mass Spectrometry (MALDI-TOF MS): a pilot study

et al. 2012

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“…After a decade of optimization of the method parameters, like the reproducibility of mass spectral profiles at different locations, the robustness to account for variations and variability in culture conditions, the application to the majority of clinically relevant bacteria, and an automated mass spectral analysis, the MALDI-TOF MS-based bacterial ID became suitable for routine use in applied laboratories (11). Commercial, user-friendly devices containing different algorithms for the classification of bacterial protein mass patterns and associated with databases including several thousand bacterial reference entries for bacterial ID, represented mainly by the Biotyper (Bruker Daltonics, Germany), Vitek MS RUO (formerly Saramis) and Vitek MS (bioMérieux, France), and Andromas (Andromas SAS, France) systems, were available (8,19).…”

mentioning

confidence: 99%

Performances of the Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Rapid Identification of Bacteria in Routine Clinical Microbiology

et al. 2012

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Rapid and cost-effective matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based systems will replace conventional phenotypic methods for routine identification of bacteria. We report here the first evaluation of the new MALDI-TOF MS-based Vitek MS system in a large clinical microbiology laboratory. This system uses an original spectrum classifier algorithm and a specific database designed for the identification of clinically relevant species. We have tested 767 routine clinical isolates representative of 50 genera and 124 species. Vitek MS-based identifications were performed by means of a single deposit on a MALDI disposable target without any prior extraction step and compared with reference identifications obtained mainly with the VITEK2 phenotypic system; if the identifications were discordant, molecular techniques provided reference identifications. The Vitek MS system provided 96.2% correct identifications to the species level (86.7%), to the genus level (8.2%), or within a range of species belonging to different genera (1.3%). Conversely, 1.3% of isolates were misidentified and 2.5% were unidentified, partly because the species was not included in the database; a second deposit provided a successful identification for 0.8% of isolates unidentified with the first deposit. The Vitek MS system is a simple, convenient, and accurate method for routine bacterial identification with a single deposit, considering the high bacterial diversity studied and as evidenced by the low prevalence of species without correct identification. In addition to a second deposit in uncommon cases, expanding the spectral database is expected to further enhance performances. Due to the dramatic increase of bacterial resistance and to the ecological cost of broad-spectrum antimicrobial therapies, rapid and accurate identification (ID) of bacteria is essential for the appropriate management of infections. Conventional identification methods require at least 4 to 12 h, and molecular methods are not suitable for large-scale routine identification.Nearly 40 years ago, chemists proposed to identify bacterial cultures via the detection of small organic molecules using mass spectrometry (2). More than 10 years later, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) allowed the detection of intact larger biomolecules, such as proteins, and was further developed for microbial ID in routine clinical laboratories (13). During the analysis process, proteins are ionized without fragmentation by the coordinated action of the laser and the small organic acids of the matrix and separated on the basis of their mass-to-charge ratios, a process which results in a characteristic mass spectral profile. Microbial ID is based on the comparison of the protein spectrum generated from intact whole bacterial cells to a database of species-specific reference protein profiles using a particular algorithm.In the mid-1990s, different groups developed their own libraries of bacter...

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MALDI‐TOF MS for Microbial Identification: Years of Experimental Development to an Established Protocol

Cited by 37 publications

References 116 publications

Taxonomic characterisation of Pseudomonas strain L48 and formal proposal of Pseudomonas entomophila sp. nov.

Taxonomic characterisation of Pseudomonas strain L48 and formal proposal of Pseudomonas entomophila sp. nov.

Discrimination of freshwater fish species by Matrix-Assisted Laser Desorption/Ionization- Time Of Flight Mass Spectrometry (MALDI-TOF MS): a pilot study

Performances of the Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Rapid Identification of Bacteria in Routine Clinical Microbiology

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