Maldi time-of-flight mass spectrometry of synthetic polymers

Nielen, Michel W. F.

doi:10.1002/(sici)1098-2787(1999)18:5<309::aid-mas2>3.0.co;2-l

Cited by 572 publications

(450 citation statements)

References 176 publications

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“…Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is commonly used for the characterization of synthetic or natural polymers 9. Information such as average molecular weights (weight and number; M w and M n ), nature and number of repeating units, and end-group determination can be deduced form MALDI-TOF-MS data.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass spectrometry for second-Generation Lignin Analysis

et al. 2012

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Matrix-Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry is evaluated as an elucidation tool for structural features and molecular weights estimation of some extracted herbaceous lignins. Optimization of analysis conditions, using a typical organic matrix, namely α-cyano-4-hydroxycinnamic acid (CHCA), in combination with α-cyclodextrin, allows efficient ionization of poorly soluble lignin materials and suppression of matrix-related ions background. Analysis of low-mass fragments ions (m/z 100–600) in the positive ion mode offers a “fingerprint” of starting lignins that could be a fine strategy to qualitatively identify principal inter-unit linkages between phenylpropanoid units. The molecular weights of lignins are estimated using size exclusion chromatography and compared to MALDI-TOF-MS profiles. Miscanthus (Miscanthus x giganteus) and Switchgrass (Panicum Virgatum L.) lignins, recovered after a formic acid/acetic acid/water process or aqueous ammonia soaking, are selected as benchmarks for this study.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass spectrometry for second-Generation Lignin Analysis

et al. 2012

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“…Mixtures of PCL hompolymers and CD-caprolactone modified conjugates were evaluated in view of the products relative content only based on the MALDI–MS spectra. Generally, compounds with different ionization efficiency in MALDI–MS will give different MS response or intensity of the corresponding MS peaks [27] which does not allow a precise quantitative measurement. In our current study we do not attempt such comparison considering that the relative amount of modified CD and homopolyester obtained by such calculation would be highly biased.…”

Section: Resultsmentioning

confidence: 99%

Cyclodextrins tethered with oligolactides – green synthesis and structural assessment

Peptu¹,

Balan-Porcăraşu²,

Šišková³

et al. 2017

Beilstein J. Org. Chem.

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Biodegradable oligolactide derivatives based on α-, β- and γ-cyclodextrins (CDs) were synthesized by a green procedure in which CDs play the role of both the initiator and the catalyst. The synthetic procedure in which CDs and L-lactide (L-LA) are reacting in bulk at relatively high temperature of 110 °C was investigated considering the structural composition of the products. The obtained products were thoroughly characterized via mass spectrometry methods with soft ionization like matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI). Liquid chromatography (LC) separation with evaporative light scattering detection (ELSD) and NMR analysis were employed in order to elucidate the structural profiles of the obtained mixtures. The results clearly demonstrate that the cyclodextrins were tethered with more than one short oligolactate chain per CD molecule, predominantly at the methylene group, through ring opening of L-LA initiated by primary OH groups.

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“…Two important parameters, including are sample preparation method and solvent selection, which played an important role in getting a better signal to noise ratio (S/N) [30,31]. One member of each class was used for optimization of the method before screening all the synthetic compounds.…”

Section: Resultsmentioning

confidence: 99%

Benzimidazole, coumrindione and flavone derivatives as alternate UV laser desorption ionization (LDI) matrices for peptides analysis

Musharraf

Bibi

Shahid

et al. 2013

Chemistry Central Journal

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BackgroundMatrix-assisted laser desorption/ionization (MALDI) is a soft ionization mass spectrometric technique, allowing the analysis of bio-molecules and other macromolecules. The matrix molecules require certain characteristic features to serve in the laser desorption/ionization mechanism. Therefore, only a limited number of compounds have been identified as ultraviolet- laser desorption/ionization (UV-LDI) matrices. However, many of these routine matrices generate background signals that useful information is often lost in them. We have reported flavones, coumarindione and benzimidazole derivatives as alternate UV-LDI matrices.ResultsThirty one compounds have been successfully employed by us as matrices for the analysis of low molecular weight (LMW) peptides (up to 2000 Da). Two peptides, bradykinin and renin substrate tetra-decapeptide were analyzed by using the newly developed matrices. The MS measurements were made after mixing the matrix solution with analyte by using dried droplet sample preparation procedures. The synthesized matrix materials showed better S/N ratios and minimal background signals for low mass range. Furthermore, pico molar concentrations of [Glu1]-fibrinopeptide B human could be easily analyzed with these matrices. Finally, BSA-digest was analyzed and identified through database search against Swiss-Prot by using Mascot.ConclusionsThese results validate the good performance of the synthesized UV-laser desorption/ionization (LDI) matrices for the analysis of low molecular weight peptides.

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Maldi time-of-flight mass spectrometry of synthetic polymers

Cited by 572 publications

References 176 publications

Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass spectrometry for second-Generation Lignin Analysis

Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass spectrometry for second-Generation Lignin Analysis

Cyclodextrins tethered with oligolactides – green synthesis and structural assessment

Benzimidazole, coumrindione and flavone derivatives as alternate UV laser desorption ionization (LDI) matrices for peptides analysis

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