MALDI Mass Spectrometric Imaging of the Nematode &lt;i&gt;Caenorhabditis elegans&lt;/i&gt;

Menger, Robert F.; Clendinen, Chaevien S.; Searcy, Louis A.; Edison, Arthur S.; Yost, Richard A.

doi:10.2174/2213235x03666150525223412

Cited by 8 publications

(6 citation statements)

References 33 publications

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“…Although UHPLC-MS/MS is able to identify many specific lipids, a large number of flies and person-hours are required. MALDI-MSI offers visualization of intensity across a sample and the benefit of mass spectra from individual fly organs (27). Therefore, we developed a MALDI-MSI approach to analyze the lipidome of organs from flies fed control or HS diets.…”

Section: Maldi-msi Reveals Temporal and Diet-dependent Changes In Lip...mentioning

confidence: 99%

“…TGs and sex pheromones were also identified on the surface of whole mounted and scanned Drosophila adults (25). MALDI-MS imaging (MALDI-MSI) has been used to identify lipid species in mouse brains with high sensitivity (26) as well as the nematode Caenorhabditis elegans (27). Studies using MALDI-MSI on sectioned Drosophila adults have revealed localization of broad sets of compounds, including phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidylglycerol, TG, phosphatidylserine (PS), and sex pheromones as well as localization of peptides and other metabolites (28).…”

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confidence: 99%

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Tissue-specific analysis of lipid species in Drosophila during overnutrition by UHPLC-MS/MS and MALDI-MSI

Tuthill

Searcy

Yost

et al. 2020

Journal of Lipid Research

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Diets high in calories can be used to model metabolic diseases, including obesity and its associated comorbidities, in animals. Drosophila melanogaster fed high-sugar diets (HSDs) exhibit complications of human obesity including hyperglycemia, hyperlipidemia, insulin resistance, cardiomyopathy, increased susceptibility to infection, and reduced longevity. We hypothesize that lipid storage in the high-sugar-fed fly’s fat body (FB) reaches a maximum capacity, resulting in the accumulation of toxic lipids in other tissues or lipotoxicity. We took two approaches to characterize tissue-specific lipotoxicity. Ultra-HPLC-MS/MS and MALDI-MS imaging enabled spatial and temporal localization of lipid species in the FB, heart, and hemolymph. Substituent chain length was diet dependent, with fewer odd chain esterified FAs on HSDs in all sample types. By contrast, dietary effects on double bond content differed among organs, consistent with a model where some substituent pools are shared and others are spatially restricted. Both di- and triglycerides increased on HSDs in all sample types, similar to observations in obese humans. Interestingly, there were dramatic effects of sugar feeding on lipid ethers, which have not been previously associated with lipotoxicity. Taken together, we have identified candidate endocrine mechanisms and molecular targets that may be involved in metabolic disease and lipotoxicity.

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Section: Maldi-msi Reveals Temporal and Diet-dependent Changes In Lip...mentioning

confidence: 99%

mentioning

confidence: 99%

Tissue-specific analysis of lipid species in Drosophila during overnutrition by UHPLC-MS/MS and MALDI-MSI

Tuthill

Searcy

Yost

et al. 2020

Journal of Lipid Research

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“…Despite this first success for flatworm research ( Ferreira et al., 2014b ; Kadesch et al., 2020 ), there is a dearth of MS-based imaging studies in parasitic nematodes, although some initial work has been conducted on C. elegans . Three preliminary studies using MALDI-MS and TOF-secondary ion MS-based platforms ( Geier et al., 2012 ; Patti et al., 2014 ; Menger et al., 2015 ) have been assessed and have showed potential for applications to nematodes. However, compared with what has been achieved in schistosomes, the spatial resolution of lipids was not sufficient for routine use in C. elegans .…”

Section: Challenges and Future Perspectivesmentioning

confidence: 99%

Helminth lipidomics: Technical aspects and future prospects

Wang

Nie

Reid

et al. 2021

Current Research in Parasitology & Vector-Borne Diseases

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“…tissues (e.g. [70–72]), developmental stages [73–76], and sub-cellular compartments). The metabolic inventory for Drosophila tissues completed by Chintapalli [77] is a good model of how such efforts might be undertaken.…”

Section: Community Resources Neededmentioning

confidence: 99%

Considerations when choosing a genetic model organism for metabolomics studies

Reed

Baer

Edison

2017

Current Opinion in Chemical Biology

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Model organisms are important in many areas of chemical biology. In metabolomics, model organisms can provide excellent samples for methods development as well as the foundation of comparative phylometabolomics, which will become possible as metabolomics applications expand. Comparative studies of conserved and unique metabolic pathways will help in the annotation of metabolites as well as provide important new targets of investigation in biology and biomedicine. However, most chemical biologists are not familiar with genetics, which needs to be considered when choosing a model organism. In this review we summarize the strengths and weaknesses of several genetic systems, including natural isolates, recombinant inbred lines, and genetic mutations. We also discuss methods to detect targets of selection on the metabolome.

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MALDI Mass Spectrometric Imaging of the Nematode <i>Caenorhabditis elegans</i>

Cited by 8 publications

References 33 publications

Tissue-specific analysis of lipid species in Drosophila during overnutrition by UHPLC-MS/MS and MALDI-MSI

Tissue-specific analysis of lipid species in Drosophila during overnutrition by UHPLC-MS/MS and MALDI-MSI

Helminth lipidomics: Technical aspects and future prospects

Considerations when choosing a genetic model organism for metabolomics studies

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