Tissue-specific analysis of lipid species in Drosophila during overnutrition by UHPLC-MS/MS and MALDI-MSI

Tuthill, Bryon F.; Searcy, Louis A.; Yost, Richard A.; Musselman, Laura Palanker

doi:10.1194/jlr.ra119000198

Cited by 27 publications

(32 citation statements)

References 80 publications

(87 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, the alterations in cardiac tissues reflected the trends in the other tissues. In total, 44 lipids were altered in the cardiac tissue in response to the intake of an HSD [ 169 ].…”

Section: Molecular Changes Following a High-sugar Diet And High-fat Dietmentioning

confidence: 99%

Phenotyping of Drosophila Melanogaster—A Nutritional Perspective

et al. 2022

View full text Add to dashboard Cite

The model organism Drosophila melanogaster was increasingly applied in nutrition research in recent years. A range of methods are available for the phenotyping of D. melanogaster, which are outlined in the first part of this review. The methods include determinations of body weight, body composition, food intake, lifespan, locomotor activity, reproductive capacity and stress tolerance. In the second part, the practical application of the phenotyping of flies is demonstrated via a discussion of obese phenotypes in response to high-sugar diet (HSD) and high-fat diet (HFD) feeding. HSD feeding and HFD feeding are dietary interventions that lead to an increase in fat storage and affect carbohydrate-insulin homeostasis, lifespan, locomotor activity, reproductive capacity and stress tolerance. Furthermore, studies regarding the impacts of HSD and HFD on the transcriptome and metabolome of D. melanogaster are important for relating phenotypic changes to underlying molecular mechanisms. Overall, D. melanogaster was demonstrated to be a valuable model organism with which to examine the pathogeneses and underlying molecular mechanisms of common chronic metabolic diseases in a nutritional context.

show abstract

Section: Molecular Changes Following a High-sugar Diet And High-fat Dietmentioning

confidence: 99%

Phenotyping of Drosophila Melanogaster—A Nutritional Perspective

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Besides CO 2 , FlyNap, with an active ingredient of triethylamine, is another anesthetic commonly used to anesthetize Drosophila 51 . Unlike CO 2 or cold anesthesia, administration of triethylamine seizes all major motor activity in flies, but maintains normal respiratory function 52 . Respirometric measurements of flies anaesthetized using triethylamine provide a baseline rate of CO 2 production and metabolism of a fly without any major movement.…”

Section: Resultsmentioning

confidence: 99%

Excessive energy expenditure due to acute physical restraint disrupts Drosophila motivational feeding response

Gordon

Mašek

2021

Sci Rep

View full text Add to dashboard Cite

To study the behavior of Drosophila, it is often necessary to restrain and mount individual flies. This requires removal from food, additional handling, anesthesia, and physical restraint. We find a strong positive correlation between the length of time flies are mounted and their subsequent reflexive feeding response, where one hour of mounting is the approximate motivational equivalent to ten hours of fasting. In an attempt to explain this correlation, we rule out anesthesia side-effects, handling, additional fasting, and desiccation. We use respirometric and metabolic techniques coupled with behavioral video scoring to assess energy expenditure in mounted and free flies. We isolate a specific behavior capable of exerting large amounts of energy in mounted flies and identify it as an attempt to escape from restraint. We present a model where physical restraint leads to elevated activity and subsequent faster nutrient storage depletion among mounted flies. This ultimately further accelerates starvation and thus increases reflexive feeding response. In addition, we show that the consequences of the physical restraint profoundly alter aerobic activity, energy depletion, taste, and feeding behavior, and suggest that careful consideration is given to the time-sensitive nature of these highly significant effects when conducting behavioral, physiological or imaging experiments that require immobilization.

show abstract

“…Mass spectrometry (MS) methods benefit from high sensitivity and requirement for low sample volumes and can be used in conjunction with isotope labeled nutrients and hormones. Combined HPLC and MS techniques were used to quantify tissue specific lipid accumulation ( Tuthill et al, 2020 ). Liquid chromatography tandem MS (LC-MS/MS) experiments can detect and quantify 13 C-labeled glucose in hemolymph drawn from flies reared on the labeled medium ( Cocuron et al, 2020 ).…”

Section: The Future Of Adipokinetic Hormone Researchmentioning

confidence: 99%

The Glucagon-Like Adipokinetic Hormone in Drosophila melanogaster – Biosynthesis and Secretion

Hughson

2021

Front. Physiol.

View full text Add to dashboard Cite

Metabolic homeostasis requires the precise regulation of circulating sugar titers. In mammals, homeostatic control of circulating sugar titers requires the coordinated secretion and systemic activities of glucagon and insulin. Metabolic homeostasis is similarly regulated in Drosophila melanogaster through the glucagon-like adipokinetic hormone (AKH) and the Drosophila insulin-like peptides (DILPs). In flies and mammals, glucagon and AKH are biosynthesized in and secreted from specialized endocrine cells. KATP channels borne on these cells respond to fluctuations in circulating glucose titers and thereby regulate glucagon secretion. The influence of glucagon in the pathogenesis of type 2 diabetes mellitus is now recognized, and a crucial mechanism that regulates glucagon secretion was reported nearly a decade ago. Ongoing efforts to develop D. melanogaster models for metabolic syndrome must build upon this seminal work. These efforts make a critical review of AKH physiology timely. This review focuses on AKH biosynthesis and the regulation of glucose-responsive AKH secretion through changes in CC cell electrical activity. Future directions for AKH research in flies are discussed, including the development of models for hyperglucagonemia and epigenetic inheritance of acquired metabolic traits. Many avenues of AKH physiology remain to be explored and thus present great potential for improving the utility of D. melanogaster in metabolic research.

show abstract

Tissue-specific analysis of lipid species in Drosophila during overnutrition by UHPLC-MS/MS and MALDI-MSI

Cited by 27 publications

References 80 publications

Phenotyping of Drosophila Melanogaster—A Nutritional Perspective

Phenotyping of Drosophila Melanogaster—A Nutritional Perspective

Excessive energy expenditure due to acute physical restraint disrupts Drosophila motivational feeding response

The Glucagon-Like Adipokinetic Hormone in Drosophila melanogaster – Biosynthesis and Secretion

Contact Info

Product

Resources

About