Malaria serology data from the Guiana shield: first insight in IgG antibody responses to Plasmodium falciparum, Plasmodium vivax and Plasmodium malariae antigens in Suriname

Labadie-Bracho, Mergiory; Genderen, Farah T. van; Adhin, Malti R.

doi:10.1186/s12936-020-03434-y

Cited by 7 publications

(6 citation statements)

References 32 publications

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“…Despite the lack of a clearly defined relationship between antigen-matched targets from the evaluated expression systems, we remain confident that microarrays utilising IVTT or purified recombinant proteins are able to produce compelling and biologically relevant data. Indeed, our data show age-dependent trends in antibody responses (typical of highly endemic populations) [1][2][3] irrespective of expression system (S3 Fig), lending weight to the applicability of either methodology in serological assays [57][58][59][60][61][62][63][64][65][66][67][68][69][70].…”

Section: Plos Onementioning

confidence: 78%

Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray

et al. 2022

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The evaluation of protein antigens as putative serologic biomarkers of infection has increasingly shifted to high-throughput, multiplex approaches such as the protein microarray. In vitro transcription/translation (IVTT) systems–a similarly high-throughput protein expression method–are already widely utilised in the production of protein microarrays, though purified recombinant proteins derived from more traditional whole cell based expression systems also play an important role in biomarker characterisation. Here we have performed a side-by-side comparison of antigen-matched protein targets from an IVTT and purified recombinant system, on the same protein microarray. The magnitude and range of antibody responses to purified recombinants was found to be greater than that of IVTT proteins, and responses between targets from different expression systems did not clearly correlate. However, responses between amino acid sequence-matched targets from each expression system were more closely correlated. Despite the lack of a clear correlation between antigen-matched targets produced in each expression system, our data indicate that protein microarrays produced using either method can be used confidently, in a context dependent manner, though care should be taken when comparing data derived from contrasting approaches.

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Section: Plos Onementioning

confidence: 78%

Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray

et al. 2022

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“…On the other hand, sera from Pv or Pfinfected individuals did not react at all against recombinant PmMSP1 19 protein (Elizardez et al, 2019). All three MSP1 19 antigens (Pf, Pv and Pm) were recently used as bound-antigens in ELISA assays that were performed to evaluate the seroprevalence of these Plasmodium species in Suriname (Labadie-Bracho et al, 2020).…”

Section: Rdt Typesmentioning

confidence: 99%

Diagnostic Methods for Non-Falciparum Malaria

Gimenez

Marques

Regiart

et al. 2021

Front. Cell. Infect. Microbiol.

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Malaria is a serious public health problem that affects mostly the poorest countries in the world, killing more than 400,000 people per year, mainly children under 5 years old. Among the control and prevention strategies, the differential diagnosis of the Plasmodium–infecting species is an important factor for selecting a treatment and, consequently, for preventing the spread of the disease. One of the main difficulties for the detection of a specific Plasmodium sp is that most of the existing methods for malaria diagnosis focus on detecting P. falciparum. Thus, in many cases, the diagnostic methods neglect the other non-falciparum species and underestimate their prevalence and severity. Traditional methods for diagnosing malaria may present low specificity or sensitivity to non-falciparum spp. Therefore, there is high demand for new alternative methods able to differentiate Plasmodium species in a faster, cheaper and easier manner to execute. This review details the classical procedures and new perspectives of diagnostic methods for malaria non-falciparum differential detection and the possibilities of their application in different circumstances.

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“…Despite the lack of a clearly defined relationship between antigen-matched targets from opposing expression systems, we remain confident that microarrays utilising IVTT or purified recombinant proteins are able to produce compelling and biologically relevant data. Indeed, our data show age-dependent trends in antibody responses (typical of highly endemic populations [1][2][3]) irrespective of expression system (Supplementary figure 2), lending weight to the applicability of either methodology in serological assays [33][34][35][36][37][38][39][40][41][42][43][44][45][46].…”

Section: Ama1 -Ivtt_1mentioning

confidence: 55%

Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray

Oulton

Obiero²,

Rodríguez

et al. 2020

Preprint

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The evaluation of protein antigens as putative serologic biomarkers of infection has increasingly shifted to high-throughput, multiplex approaches such as the protein microarray. In vitro Transcription/Translation (IVTT) systems (a similarly high-throughput protein expression method) are already widely utilised in the production of protein microarrays, though purified recombinant proteins derived from more traditional whole cell based expression systems also play an important role in biomarker characterisation. Here we have performed a side-by-side comparison of antigen-matched protein targets from an IVTT and purified recombinant system, on a protein microarray. The magnitude and range of antibody responses to purified recombinants was found to be greater than that of IVTT proteins, and responses between targets from opposing expression systems did not clearly correlate. However, responses between amino acid sequence-matched targets from each expression system were more closely correlated. Despite the lack of a clearly defined relationship between antigen-matched targets produced in each expression system, our data indicate that protein microarrays produced using either method can be used confidently, in a context dependent manner, though care should be taken when comparing data derived from contrasting approaches.

show abstract

Malaria serology data from the Guiana shield: first insight in IgG antibody responses to Plasmodium falciparum, Plasmodium vivax and Plasmodium malariae antigens in Suriname

Cited by 7 publications

References 32 publications

Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray

Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray

Diagnostic Methods for Non-Falciparum Malaria

Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray

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