Malacological survey in a bottle of water: A comparative study between manual sampling and environmental DNA metabarcoding approaches

Effects of the invasive aquatic snail Potamopyrgus antipodarum (Gray, 1853) on ecosystem properties and services

“…The reduction in wild invertebrate populations of the native fauna supposes a direct deterioration in the provisioning services (e.g., reduction in invertebrate animals for food supply or/ and as supporting vertebrate fauna), which can also cause a reduction in cultural services (i.e., reduction of bait for angling activities). However, the extreme density reached by the NZMS may have an indirect positive effect on regulating ecosystem services when the affected native species are vectors of diseases such as Bulinus truncatus (Audouin, 1827) and lymnaeids, gastropods which transmit liver flukes among them schistosomes (Jones et al, 2015;Mulero et al, 2021). Several studies show that the NZMS may facilitate some native invertebrates (Schreiber et al, 2002;Brenneis et al, 2010).…”

“…Pest and disease control can be improved by the NZMS by means of competition with native snail hosts of pathogens or as an unsuitable intermediate host for native pathogens (Jones et al, 2015;Marszewska et al, 2018;Mulero et al, 2021). The NZMS presents a potential dilution effect against native trematode parasites, consequently reducing infection level (both prevalence and abundance) of native intermediate and definitive host species, such as gastropods and vertebrates.…”

Environmental DNA in human and veterinary parasitology - Current applications and future prospects for monitoring and control

“… Freshwater qPCR ( Rudko et al, 2020 ) Snail-host Galba truncatula Freshwater LAMP ( Davis et al, 2020 ) Parasite + Snail-host Fascila + Austropeplea tomentosa Freshwater Multiplex qPCR ( Rathinasamy et al, 2021 ) Snail host Galba truncatula Freshwater PCR ( Jones et al, 2021 ) Snail host Gastopoda Freshwater Metabarcoding ( Mulero et al, 2021 ) Reservoir host Vertebrata Mosquitos (iDNA) Metabarcding ( Hopken et al, 2021 ) …”

“…A fundamental challenge in relation to the application of eDNA methods for many species of parasites and vectors, is that the genes sequenced and available in public reference databases, i.e. NCBI and BOLD, are highly patchy ( Krol et al, 2019 ; Mulero et al, 2021 ). Often only a single locus (e.g.…”

“…Schistosoma mansoni , S. haematobium and Ascaris suum ( Berriman et al, 2009 ; Jex et al, 2011 ; Young et al, 2012 ). Furthermore, the lack of database sequence coverage also hampers the full exploitation of eDNA metabarcoding approaches in parasitology as it can be difficult to assign many of the sequences generated from HTS (see Box 1 ) to genus or species level ( Mulero et al, 2021 ). Recently, eDNA reference databases are being built as seen in the Danish project ‘DNAmark’, where DNA from animals and plants occurring in Denmark, including Fasciola hepatica liver flukes and their intermediate host snail Galba truncatula , is sequenced and made publicly available enabling future eDNA-based environmental monitoring ( Margaryan et al, 2021 ).…”