Making one cell from two

Miller, Jeffrey B.

doi:10.1038/377575a0

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…The recent publication [Yagami-Hiromasa et al, 1995] identifying myoblast transcripts with homologies to guinea pig fertilin-␣ and ␤ generated considerable excitement [Miller, 1995] because it suggested a common mechanism between sperm-egg fusion and myotube formation. The evidence that meltrin-␣ is involved in fusion is suggestive, relying on time of expression and antisense suppression studies.…”

Section: Discussionmentioning

confidence: 99%

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Expression of meltrin-α mRNA is not restricted to fusagenic cells

Harris¹,

Murrills²,

Komm³

1997

J. Cell. Biochem.

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Meltrin-alpha is a myoblast gene product reported to be required for cell fusion [Yagami-Hiromasa et al. (1995): Nature 377:652-656]. Because Northern blots revealed expression only in muscle and bone, the suggestion was made that meltrin-alpha is expressed exclusively by fusagenic cells in these tissues (myoblast and osteoclast). We studied expression of meltrin-alpha mRNA in a panel of tissues and cell lines using the polymerase chain reaction and found it widely expressed. Meltrin-alpha mRNA was readily detected in the osteoblast, the most abundant cell type in bone. In situ hybridization analysis on sections of neonatal mice revealed high levels of expression in the trabecular meshwork of long bones, the basal regions of the dermis and its underlying mesenchyme. We conclude that expression of meltrin-alpha mRNA is not restricted to fusagenic cells and that, in bone, the osteoblast is the major source.

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Section: Discussionmentioning

confidence: 99%

“…The evidence that meltrin-␣ is involved in fusion is suggestive, relying on time of expression and antisense suppression studies. But, as remarked by Miller [1995], a definitive role for meltrin-␣ in fusion has not yet been proven. As in sperm-egg interaction, other processes (such as adhesion) precede the myoblast fusion event.…”

Section: Discussionmentioning

confidence: 99%

Expression of meltrin-α mRNA is not restricted to fusagenic cells

Harris¹,

Murrills²,

Komm³

1997

J. Cell. Biochem.

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“…This would explain why disintegrin-like peptides appear not to affect the ability of sperm to fuse with oocytes until binding itself is inhibited. Interestingly, recent evidence suggests that a-meltrin, a homologue of the M subunit of PH-30 in mouse myoblasts, plays a role in myotube formation (18), but whether it is at the fusion step or some prior adhesion step is unclear (28). A polyclonal antibody against a-meltrin does not block myoblast fusion (18).…”

Section: Fusion Of Sperm and Oocytementioning

confidence: 99%

The mammalian acrosome reaction: Gateway to sperm fusion with the oocyte?

Allen

Green

1997

BioEssays

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Mammalian sperm undergo discharge of a single, anterior secretory granule following their attachment to the zona pellucida surrounding the oocyte. This secretory discharge is known for historical reasons as the acrosome reaction. It fulfils a number of purposes and without it, sperm are unable to penetrate the zona pellucida and fuse with the oocyte. In this review, we focus on the role of the acrosome reaction in the development of fusion competence in sperm. Any naturally occurring membrane fusion has two major sequential steps: a docking or adhesion step, in which two membranes adhere, and a fusion step, in which their lipid bilayers are destabilized and merged and a cellular compartment is either created or destroyed. Recent evidence suggests that there is an important role for oocyte integrins and sperm-bound disintegrins in mammalian sperm/oocyte adhesion and fusion. The fusion mechanism employed by sperm remains poorly understood, however, and circumstantial evidence suggests it is more complex than the interaction between a single protein species and its target. Sperm/oocyte fusion is probably the most accessible eukaryotic model for intercellular fusion currently available, partly because it is temporally separated from gene expression. Elucidation of the mechanism of sperm/oocyte fusion may throw light on the mechanism of other intercellular fusions such as myoblast fusion, and the evolutionary relationship of intercellular membrane fusion to intracellular membrane fusion.

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Cell-Cell Fusion, Chemotaxis and Metastasis

Parris

2015

Intercellular Communication in Cancer

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Making one cell from two

Cited by 7 publications

References 16 publications

Expression of meltrin-α mRNA is not restricted to fusagenic cells

Expression of meltrin-α mRNA is not restricted to fusagenic cells

The mammalian acrosome reaction: Gateway to sperm fusion with the oocyte?

Cell-Cell Fusion, Chemotaxis and Metastasis

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