Expression of meltrin-α mRNA is not restricted to fusagenic cells

Abstract

Meltrin-alpha is a myoblast gene product reported to be required for cell fusion [Yagami-Hiromasa et al. (1995): Nature 377:652-656]. Because Northern blots revealed expression only in muscle and bone, the suggestion was made that meltrin-alpha is expressed exclusively by fusagenic cells in these tissues (myoblast and osteoclast). We studied expression of meltrin-alpha mRNA in a panel of tissues and cell lines using the polymerase chain reaction and found it widely expressed. Meltrin-alpha mRNA was readily detected in the osteoblast, the most abundant cell type in bone. In situ hybridization analysis on sections of neonatal mice revealed high levels of expression in the trabecular meshwork of long bones, the basal regions of the dermis and its underlying mesenchyme. We conclude that expression of meltrin-alpha mRNA is not restricted to fusagenic cells and that, in bone, the osteoblast is the major source.

Molecular characterization and expression analysis of ADAM12 during chicken embryonic development

“…The tissues that are ADAM12 positive derive exclusively from the neural tube, the neural crest or the mesoderm. Our results confirm and extend the studies of ADAM12 in other species (Harris et al. 1997; Kurisaki et al.…”

Endogenous Meltrin Is Ubiquitously Expressed and Associated with the Plasma Membrane but Exogenous Meltrin Is Retained in the Endoplasmic Reticulum

“…mRNA and protein were already present in proliferating myoblasts and persisted throughout differentiation, and thus its expression was not coordinated with that of myogenin or certain muscle-specific genes. Considering the reports that meltrin a is expressed in a variety of cells and tissues (29)(30)(31)(32), and our finding that 10T1/2 fibroblasts also contain meltrin a mRNA and protein, the constitutive expression of meltrin ot in C2 cells seems to be plausible. Thus, meltrin a is likely to play general roles in these cells and tissues in addition to its specific roles in myogenesis.…”

“…The previous study showed that the amount of meltrin a mRNA was very low in proliferating C2 skeletal muscle myoblasts and remarkably increased in response to the induction of differentiation in a manner similar to in the case of myogenin mRNA (17). However, its mRNA and protein have been detected in several human tissues and tumor cell lines (29,30), and mouse tissues during development (31,32). We reexamined the amount of meltrin a mRNA during the differentiation of C2 cells and in 10T1/2 fibroblasts by quantify!…”

The ADAMs family of metalloproteases: multidomain proteins with multiple functions

“…For example, most of the mature form of ADAM15 is resistant to trypsinization treatment, indicating a predominantly intracellular pool (Lum et al 1998;Howard et al 2000). Furthermore, the metalloprotease activity of ADAMs 10, 17, and 19 can occur within intracellular compartments (Gupta et al 1996) 2 (Lin et al 2001) 3 (Yoshiyama et al 1997) 4 (Weskamp et al 1996) 5 (Hotoda et al 2002) 6 (Chantry and Glynn 1990) 7 (Yavari et al 1998) 8 (Sagane et al 1998) 9 (Katagiri et al 1995) 10 (Wu et al 1997) 11 (Yagami-Hiromasa et al 1995) 12 (Harris et al 1997) 13 14 15 (Patel et al 1998) 16 (Frayne et al 2002) 17 18 (Poindexter et al 1999) 19 (Harada et al 2000) 20 (Cal et al 2000) 21 (Roberts et al 1999) 22 (Howard et al 2000) 23 (Howard et al 2001) 24 25 26 MP Active refers to either predicted (p) or demonstrated/ published (d) activity based on the amino acid sequence of the catalytic active site. (Skovronsky et al 2000;Shirakabe et al 2001).…”