Making Green Polymers Even Greener:Towards Sustainable Production of Polyhydroxyalkanoates from Agroindustrial By-Products

Gómez, José; Méndez, Beatriz S.; Nikel, Pablo I.; Pettinari, M. Julia; Prieto, María Auxiliadora; Silva, Luiziana Ferreira da

doi:10.5772/31847

Cited by 33 publications

(33 citation statements)

References 108 publications

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“…Yet, chlorinated solventseven if recycling is performed, are not desirable due to both environmental and health reasons. Therefore, efforts should be spent on developing alternative purification methods, either based on greener solvents, enzymatic digestion of the cell material or autolysis systems 2,15,29 .…”

Section: Characterization Of the Functionalized Mcl-pha Producedmentioning

confidence: 99%

“…Nevertheless, its increasing cost 14 and the fact that this limited substrate should be preferentially used for food applications led us to explore cheaper and more sustainable alternatives, such as food wastes. Like agro-wastes, food wastes are very diverse and readily available, which renders them very interesting candidates for biopolymer production [15][16][17] . In a previous work, we designed bioprocesses for the synthesis of mcl-PHA using hydrolyzed fruit pomace (pressing residues) for the growth phase, and waste frying oil for the mcl-PHA accumulation phase 18 .…”

Section: Introductionmentioning

confidence: 99%

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Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids

Follonier

Riesen²,

Zinn

2015

Chem.Biochem.Eng.Q.

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Section: Characterization Of the Functionalized Mcl-pha Producedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids

Follonier

Riesen²,

Zinn

2015

Chem.Biochem.Eng.Q.

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“…Relevant examples in this sense include (but are certainly not limited to) the production of ethanol (31,58,69), succinate (64), D-lactate (45), and polyhydroxyalkanoates (24,40), often by using redox and/or regulatory E. coli mutants as the biocatalyst. These metabolic engineering approaches underscore the need for a complete understanding of the cell physiology and metabolic network operativity under anoxic growth conditions.…”

mentioning

confidence: 99%

Manipulation of the Anoxic Metabolism in Escherichia coli by ArcB Deletion Variants in the ArcBA Two-Component System

Bidart

Ruiz

Almeida

et al. 2012

Appl Environ Microbiol

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Bioprocesses conducted under conditions with restricted O 2 supply are increasingly exploited for the synthesis of reduced biochemicals using different biocatalysts. The model facultative anaerobe Escherichia coli has elaborate sensing and signal transduction mechanisms for redox control in response to the availability of O 2 and other electron acceptors. The ArcBA two-component system consists of ArcB, a membrane-associated sensor kinase, and ArcA, the cognate response regulator. The tripartite hybrid kinase ArcB possesses a transmembrane, a PAS, a primary transmitter (H1), a receiver (D1), and a phosphotransfer (H2) domain. Metabolic fluxes were compared under anoxic conditions in a wild-type E. coli strain, its ⌬arcB derivative, and two partial arcB deletion mutants in which ArcB lacked either the H1 domain or the PAS-H1-D1 domains. These analyses revealed that elimination of different segments in ArcB determines a distinctive distribution of D-glucose catabolic fluxes, different from that observed in the ⌬arcB background. Metabolite profiles, enzyme activity levels, and gene expression patterns were also investigated in these strains. Relevant alterations were observed at the P-enol-pyruvate/pyruvate and acetyl coenzyme A metabolic nodes, and the formation of reduced fermentation metabolites, such as succinate, D-lactate, and ethanol, was favored in the mutant strains to different extents compared to the wild-type strain. These phenotypic traits were associated with altered levels of the enzymatic activities operating at these nodes, as well as with elevated NADH/NAD ؉ ratios. Thus, targeted modification of global regulators to obtain different metabolic flux distributions under anoxic conditions is emerging as an attractive tool for metabolic engineering purposes.A noxic fermentation of different carbon sources by Escherichia coli is increasingly gaining momentum in biotechnological setups designed to obtain reduced biochemicals. Relevant examples in this sense include (but are certainly not limited to) the production of ethanol (31, 58, 69), succinate (64), D-lactate (45), and polyhydroxyalkanoates (24, 40), often by using redox and/or regulatory E. coli mutants as the biocatalyst. These metabolic engineering approaches underscore the need for a complete understanding of the cell physiology and metabolic network operativity under anoxic growth conditions. In fact, the relative lack of knowledge on the cellular wiring of these regulatory networks under conditions relevant to both laboratory and industrial applications represents a hurdle that has to be overcome for the efficient design of industrial processes. Metabolic fluxes through the central carbon pathways constitute the backbone of cell metabolism and represent the in vivo reaction rates of cognate enzymatic steps (62). The observed fluxome is the phenotypic consequence of both gene transcription and translation, as well as of the enzymatic activity and the regulation exerted at the metabolite level (48). Fluxome analysis is thus a useful approach to...

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“…Metabolic networks and carbon flux play a central role in PHA production, and a number of core cellular metabolic enzymes have been manipulated in attempts to improve PHA yields in various bacterial species (7,14,15). Signal transduction systems associated with the detection of extracellular conditions and the coordination of central metabolic pathways involved have also been implicated in PHA production control.…”

mentioning

confidence: 99%

“…Several comprehensive recent reviews are available in the field of microbial PHA synthesis (5)(6)(7). In summary, PHAs are condensation polyesters of various hydroxyalkanoic acids, with 2 major classes identified based on the respective chain lengths of the monomer units that make up the polymers.…”

mentioning

confidence: 99%

GacS-Dependent Regulation of Polyhydroxyalkanoate Synthesis in Pseudomonas putida CA-3

Ryan

O’Leary

O’Mahony

et al. 2013

Appl Environ Microbiol

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To date, limited reports are available on the regulatory systems exerting control over bacterial synthesis of the biodegradable polyester group known as polyhydroxyalkanoates (PHAs). In this study, we performed random mini-Tn5 mutagenesis of the Pseudomonas putida CA-3 genome and screened transconjugants on nitrogen-limited medium for reduced PHA accumulation phenotypes. Disruption of a GacS sensor kinase in one such mutant was found to eliminate medium-chain-length PHA production in Pseudomonas putida CA-3. Recombinant expression of wild-type gacS from a pBBRgacS vector fully restored PHA accumulation capacity in the mutant strain. PCR-based screening of the P. putida CA-3 genome identified gene homologues of the GacS/GacA-rsm small RNA (sRNA) regulatory cascade with 96% similarity to published P. putida genomes. However, reverse transcription-PCR (RT-PCR) analyses revealed active transcription of the rsmY and rsmZ sRNAs in gacS-disrupted P. putida CA-3, which is atypical of the commonly reported Gac/Rsm regulatory cascade. Quantitative real-time RT-PCR analyses of the phaC1 synthase responsible for polymer formation in P. putida CA-3 indicated no statistically significant difference in transcript levels between the wild-type and gacS-disrupted strains. Subsequently, SDS-PAGE protein analyses of these strains identified posttranscriptional control of phaC1 synthase as a key aspect in the regulation of PHA synthesis by P. putida CA-3.

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Making Green Polymers Even Greener:Towards Sustainable Production of Polyhydroxyalkanoates from Agroindustrial By-Products

Cited by 33 publications

References 108 publications

Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids

Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids

Manipulation of the Anoxic Metabolism in Escherichia coli by ArcB Deletion Variants in the ArcBA Two-Component System

GacS-Dependent Regulation of Polyhydroxyalkanoate Synthesis in Pseudomonas putida CA-3

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